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53results about How to "Does not cause rejection" patented technology

Implanted flexible neural microelectrode comb, and preparation method and implanting method thereof

The invention provides an implanted flexible neural microelectrode comb, and a preparation method and an implanting method thereof. The flexible neural microelectrode comb is mainly composed of a flexible substrate layer, a flexible insulation layer and a metal connection wire layer arranged between the flexible substrate layer and the flexible insulation layer; the flexible neural microelectrode comb comprises a comb-like structure, a grid structure, a solid structure and a welding pad connected in sequence; electrode sites are arranged on the comb-like structure; welding points are arranged on the welding pad; the metal connection wire layer is composed of metal connection wires connecting the electrode sites and the welding points; and the flexible insulation layer is arranged on the surfaces of the electrode sites and the welding points. The flexible neural microelectrode comb prepared according to the method provided by the invention has a wire-grid-plane gradual changing structure, and thus is improved in mechanical stability during a shape changing process. The mechanical property of the implanted flexible neural microelectrode comb is matched with a brain tissue, the implanting areas is small, an inflammatory response of the brain is avoided, and electroencephalogram signals can be stably tracked and measured in a multi-point manner for a long time.
Owner:BEIJING BCIFLEX MEDICAL TECH CO LTD

Decellularized matrix-source tissue engineering scaffold and preparation method and application thereof

The invention relates to a decellularized matrix-source tissue engineering scaffold and a preparation method and an application thereof; the decellularized matrix-source tissue engineering scaffold takes a treated animal membrane material as a biological membrane base material, and the surface of the biological membrane base material is attached with a collagen loose layer. The preparation method of the decellularized matrix source tissue engineering scaffold comprises the process steps of degreasing, decellularizing, antigen removal, cross-linking fixation, collagen extraction, collagen compositing and the like. The flexible and smooth animal membrane material is used as the biological membrane base material, and the defects that a pure collagen scaffold has poor mechanical properties and too fast degradation rate are overcome; with cooperation of the collagen loose layer, a good microenvironment is provided for tissue regenerative repair and cell growth; after being implanted into a body, the decellularized matrix-source tissue engineering scaffold material is gradually degraded along with repairing of defect tissues, also has controllable degradation time, does not exist as a permanent foreign matter, and has no any residual toxicity.
Owner:广州市美昊生物科技有限公司 +1

Nanoparticle-loaded microsphere system for injection and preparation method of system

The invention discloses a nanoparticle-loaded microsphere system for injection. Drugs are coated in nanoparticles, and the nanoparticles are coated in a microsphere, wherein the nanoparticles are made from material selected from one of albumin, chitosan and a polylactic acid-glycolic acid polymer, and the microsphere is made from either the chitosan or the polylactic acid-glycolic acid polymer. Furthermore, the invention provides a preparation method of the microsphere system, the nanoparticles are prepared through a desolvation and emulsification cross-linking method, a cross-linking method or a dual-emulsification method, and the prepared nanoparticles are further treated by the dual-emulsification method or a spray-drying method so as to obtain the microsphere. The nanoparticle-loaded microsphere system disclosed by the invention is free from a rejection reaction, and is applicable to parenteral administration; the microsphere system can adapt to natures of various drugs, which further enhances a controlled-release effect and controllability, the microsphere system can achieve a graded successive targeting effect by modifying two layers of materials inside and outside.
Owner:NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE

Use of mesenchymal stem cell and preparation method thereof

The invention discloses a mesenchymal stem cell, which is used as a target cell for gene treatment, and used for a carrier of expressing, storing and releasing a transgenic drug. An exogenous promoter and a target gene are led to the mesenchymal stem cell by a virus carrier; and the target gene is integrated into a genome of a host cell, so as to stabilize that the endogenously expressed target gene is not lost and used for gene treatment. It is fully verified that the mesenchymal stem cell can be used as a storage cavern for expressing, storing and releasing a target drug, and has the characteristics of being efficient, safe, reliable and the like by an insulin gene treatment experiment.
Owner:JILIN UNIV

Method for restoring totipotency of mesenchymal stem cell

The invention discloses a culture method for restoring totipotency of a mesenchymal stem cell. The method for restoring totipotency of a mesenchymal stem cell, provided by the invention, comprises the following steps: 1) performing hanging-drop culture on an in-vitro mesenchymal stem cell with reduced totipotency, thus obtaining a hanging-drop cultured cell; and 2) further performing suspension culture on the hanging-drop cultured cell, thus realizing totipotency restoration of the mesenchymal stem cell. The experiment of the invention proves that, according to the method for restoring totipotency of a mesenchymal stem cell (specifically 3D culture), after the mesenchymal stem cell (in-vitro mesenchymal stem cell with reduced totipotency) subcultured multiple times is subjected to 3D culture treatment, the increased volume is gradually restored, the cell viability is enhanced, the cloning capability and differentiation capability are enhanced, and the paracrine action is also improved, thereby activating the aged mesenchymal stem cell and restoring the characteristics of the stem cell.
Owner:SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV

Preparation method of nano medicine controlled release system and product and application of system

The invention discloses a preparation method of a nano medicine controlled release system. The preparation method comprises the following steps: (1) preparing a nanoscale red blood cell membrane vesicle, (2) preparing photosensitive drug-loaded graphite oxide, (3) preparing a target molecule and (4) preparing the nano drug controlled release system. According to the method, a protein crown of a nano carrier due to coating by certain in-vivo proteins can be avoided by embedding of a red blood cell vesicle; the activity of the target molecule is guaranteed; the red blood cell membrane vesicle exists in a human body, has good biocompatibility and no toxic and / or side effects and does not cause a rejection reaction; the red blood cell embedded vesicle can effectively reduce surface free energyof graphite oxide and improve dispersity of the nano drug controlled release system; graphite oxide is absorbed with a photosensitizer, namely indocyanine green; and together with photo-thermal treatment, an antitumor effect of the nano drug controlled release system can be further improved.
Owner:THE THIRD XIANGYA HOSPITAL OF CENT SOUTH UNIV

Transgenic mesenchyma stem cell for curing radiation enteritis and method of producing the same

The invention discloses a transgenic mesenchymal stem cell which can treat radiation enteritis and a preparation method. The CXCR4 protein can be expressed highly on the surface of transgenic mesenchymal stem cell; under the chemotaxis of stromal cell derived factor which is released from the injured intestine, the homing and colonization of a large amount of transgenic mesenchymal stem cells can promote proliferation and repair of the intestinal mucosa and inhibit apoptosis; the radiation enteritis is cured. The steps of the preparation method of the transgenic mesenchymal stem cell are that : (1)the separation and cultivation of mesenchymal stem cell; (2) the preparation of CXCR4 recombinant adenovirus: full length cDNA of CXCR4 is amplified by RT-PCR; the construction of CXCR4 recombinant shuttle plasmid and skeleton plasmid; the CXCR4 recombinant adenovirus is packaged, amplified, purified and determined by titer in HEK293T cell; (3) the transfect ion of mesenchymal stem cell by using CXCR4 recombinant adenovirus.
Owner:NANJING UNIV

Cervical spondylotic myelopathy experimental animal model and making method thereof

The invention relates to a cervical spondylotic myelopathy (CSM) experimental animal model and a making method thereof, belonging to the technical field of osteopathic medicine experimental animal models. According to the technical scheme of the invention, a living rabbit, goat, dog, miniature pig or experimental mouse is used as a model body, a compression nail is placed at C3 cervical vertebra body of the rabbit, goat, dog, miniature pig or experimental mouse, and developing biomaterial is filled inside the filling hole of the compression nail. The making method of the CSM experimental animal model comprises the following steps of: making a hole at the C3 cervical vertebra body, and placing the compression nail into the hole to cause spinal cord ventral compression. The CSM experimental animal model provided by the invention has the following benefits that the C3 cervical vertebra is selected as the vertebral body where the compression nail is placed, which completely breaks the domestic and abroad traditional mode of compression at C4 or C5 cervical vertebra, is a new breakthrough in CSM experimental animal model making and achieves an ideal experimental effect; and the surgical operation is simple and safe, and the change of CSM after long-term chronic compression can be studied in various imaging techniques at the same time.
Owner:申勇

Personalized bone fracture plate and manufacturing method thereof

The invention discloses a personalized bone fracture plate and a manufacturing method thereof. The bone fracture plate made from titanium or titanium alloy through 3D printing is in fit contact with the shape of a patient bone, and the surface of the bone fracture plate is provided with a hydroxyapatite film. The manufacturing method comprises the steps that 1, CTA scanning technology or MRI technology is adopted for carrying out three-dimensional radiographing on the paint bone, and three-dimensional data of the patient bone is obtained; 2, computer aided design is used for displaying the patient bone and determining the shape, size and the corresponding connection way of the bone fracture plate; 3, personalized bone fracture plate data is output, and a metal powder selective laser melting 3D printer is used for printing the bone fracture plate, wherein the adopted titanium or titanium alloy is of the particle diameter of 15-45 micrometers and is in a spherical shape; 4, vacuum heat treatment is carried out on the bone fracture plate obtained through 3D printing; 5, the hydroxyapatite film is produced on the surface of the bone fracture plate.
Owner:广州雄俊智能科技有限公司

Fabrication method for fracture fixator

The invention discloses a fabrication method for a fracture fixator, which includes the following steps: scanning the fracture and the corresponding healthy-side part of a patient, and acquiring initial data; adopting three-dimensional software to convert the initial data into a three-dimensional model, so that a skeleton model at the fracture and the corresponding healthy-side part of the patient is obtained; utilizing the three-dimensional software to carry out a fixation analysis on the skeleton model at the fracture and design a fracture fixator model; preparing bioactive artificial bone material; converting the designed fracture fixator model into a rapid prototyping file format, and feeding the prepared bioactive artificial bone material as prototyping material into a rapid prototyping machine to produce the fracture fixator. The invention has the advantage that the rapid prototyping technique, the three-dimensional computer design and the bioactive artificial bone material are combined to fabricate degradable fracture fixators adapted to different parts and different degrees of individualized shape designs.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA

Yak lactoprotein polypeptide nutrition powder applicable to rehabilitation of people after operations and preparation method of powder

The invention discloses yak lactoprotein polypeptide nutrition powder applicable to rehabilitation of people after operations and a preparation method of the powder. The powder comprises the following main components: 20-24% of yak lactoprotein polypeptide powder, 6-10% of sodium caseinate, 18-20% of whey protein, 8-10% of defatted milk powder, 1-2% of bovine coloctrum, 0.02-0.03% of arginine, 0.11-0.14% of taurine, 8-12% of glucose, 1.1-1.5% of a silverweed cinquefoil extract, 0.1-1.4% of a lily extract, 1.5-2.5% of apple powder, 0.1-0.3% of vitamin C, 0.01-0.02% of vitamin E, 0.004-0.008% of group-B vitamins, 8-12% of polydextrose, 5-9% of fructo-oligose and 8-10% of maltodextrin. Through the organic combination of nutrients, energy and nutrient substances required by people after the operations can be comprehensively supplemented, and substances which are required by the people after the operations for the rehabilitation as soon as possible are provided.
Owner:GANNAN BEIYITE BIOLOGICAL SCI & TECH

Thermosensitive polymer vesicle and preparation method and application thereof

The invention relates to a thermosensitive polymer vesicle and a preparation method and an application thereof. The thermosensitive polymer vesicle includes a vesicle body assembled by amphiphilic thermosensitive block-containing polymers, and a biotin-modified Biotin-CD146 monoclonal antibody and streptavidin (SA) targeting to periodontal ligament stem cells (PDLSCs) and combined on the vesicle body. The thermosensitive polymer vesicle can be used as a hydrogen sulfide vector for application. Compared with the prior art, the thermosensitive polymer vesicle has the PDLSCs targeting property, can improve the proliferation and differentiation ability of the PDLSCs, promotes periodontal tissue remodeling under an inflammation environment, improves the orthodontic curative effect and safety inan inflammation state, and provides technical support for clinical application of gas molecules.
Owner:TONGJI UNIV

Polymer vesicle capable of promoting wound healing, and preparation method and application of polymer vesicle capable of promoting wound healing

The invention relates to a polymer vesicle capable of promoting wound healing. The polymer vesicle capable of promoting wound healing comprises a vesicle prepared through assembly of antibacterial peptide polymers, and a hydrogen sulphide donor combined to the vesicle, wherein the antibacterial peptide polymer is polycaprolactone-block-poly[lysine-random-(S-aroyl oxime)] and a derivative thereof.A preparation method of the polymer vesicle capable of promoting wound healing comprises the steps of firstly, enabling polycaprolactone and alpha-amino acid N-carboxyanhydride (NCA) to be subjected to a ring-opening polymerization reaction to synthetize the antibacterial peptide polymers, then enabling the hydrogen sulphide donor to be combined with the polymer vesicle, and finally, enabling thepolymers to be self-assembled to obtain the polymer vesicle which concurrently has antibacterial activity and functions of the hydrogen sulphide donor. The polymer vesicle disclosed by the invention has excellent antibacterial activity on gram-positive bacteria and gram-negative bacteria, and can slowly release hydrogen sulphide for 12 hours in an aqueous solution containing cysteine. The experiment on animals proves that compared with a commercialized wound repair spray, the vesicle disclosed by the invention can notably promote wound healing of a mouse suffering from diabetes.
Owner:TONGJI UNIV

Skull repair material and preparation method for same

The invention discloses an artificial skull repair material, and belongs to the technical field of orthopaedic repair materials. The artificial skull repair material comprises the following components in parts by weight: 15 to 29 parts of bone cement, 11 to 35 parts of a 3D (3-dimensional) titanium mesh, 11 to 17 parts of a polylactic acid composite material chitin and 15 to 21 parts of polyetheretherketone. The invention further provides a preparation method for the artificial skull repair material. A skull structure with a required radian can be rapidly prepared by adopting a 3D printing technology. The skull repair material comprising the components in parts by weight is processed to prepare skull prosthesis with any required radian by adopting the 3D printing technology, and is high in biocompatibility and low in cost, the advantages of materials such as the bone cement and the 3D titanium mesh are integrated, the shortcomings of a common skull repair material in terms of mechanical performance, processibility and consistency are effectively overcome, and meanwhile, side effects in clinical applications are obviously reduced.
Owner:QINGDAO BAIZHONG CHEM TECH

Biologically composite artificial bone and its preparing process

The invention relates to a biological composite artificial bone and its preparation process. The artificial bone is prepared by poly lactic acid, hydroxyapatite(or calcium phosphate) and OPG protein with weight ratio of 180-250 : 100-160 : 0.6-1. The invention mixes poly lactic acid, hydroxyapatite(or calcium phosphate) and OPG protein only once by emulsion cross blend method without changing their chemical structure. The adopted ethyl acetate and absolute ethyl alcohol have no harm to the human body, and the hydroxyapatite(or calcium phosphate) bone conduction function and the osteoclast genesis and activation of the OPG protein are kept. It can also initiate osteoclast apoptosis. The compound product is a biological compound artificial bone for treating bone defection and osteoporosis in clinical practice.
Owner:上海盈康医药科技有限公司

Rapid hemostatic

The invention relates to a rapid hemostatic. Preparation raw materials of the rapid hemostatic comprise microporous starch, calcium ions or zinc ions, chitosan or chitin. In every 100 g of the microporous starch, the addition amount of the chitosan or chitin is 1 to 50 g, the addition amount of the calcium ions is 10 to 500 micromoles, or the addition amount of the zinc ions is 6 to 80 micromoles. When the rapid hemostatic disclosed by the invention is not used, the temperature of skin at a wound cannot be increased obviously, and sharp temperature rise of the wound during hemostasis can be effectively prevented, so that pains of a patient due to the fact that the temperature of the wound is increased when an existing hemostatic is used are avoided, and the novel hemostatic can be used for hemostasis of internal and external wounds under various middle and small arteries.
Owner:NORTHWEST UNIV(CN)

Reg4 protein and application thereof in resisting salmonella enteritidis infection

The invention provides an application of an active factor, which is characterized in that the active factor is Reg4 or a substance with an amino acid sequence similar to that of Reg4 by about three amino acid sequences; the application comprises at least one of the following applications: A, preparing a medicine for treating and / or relieving diseases caused by salmonella typhimurium infection; and B, preparing a medicine for treating and / or relieving complications caused by salmonella typhimurium infection.
Owner:SHANGHAI INST OF PEDIATRIC RES

Preparation method and application of a polymer vesicle with sugar sponge function

The present invention relates to a polymer vesicle with the function of sugar sponge and its preparation method and application. Concanavalin on the above; its preparation method is: at first synthesizing the amphiphilic sugar-based polymer with at least initiator and polymerized monomer, then making the amphiphilic sugar-based polymer assemble into polymer vesicles, and finally Concanavalin is combined with polymer vesicles to form sugar-responsive polymer vesicles; the sugar-responsive polymer vesicles of the present invention can respond to changes in the concentration of glucose or mannose in the external environment and are not affected by the pH value. It has good stability and can be used as a buffer for glucose or mannose or a non-drug buffer for treating diabetes.
Owner:TONGJI UNIV

Protein sequence capable of inhibiting malignant tumors and application

The invention belongs to the field of gene engineering, particularly relates to a protein sequence capable of efficiently inhibiting malignant tumors, and further relates to a gene sequence for encoding the protein sequence and application of the protein sequence. The application of the protein sequence as shown in SEQ ID NO.1 in preparation of a preparation for inhibiting the malignant tumors isthe key protected content of the invention. The protein sequence as shown in SEQ ID NO.1 is expressed by a gene sequence as shown in SEQ ID NO.2; and in addition, the gene sequence as shown in SEQ IDNO.2 is also the protected content of the invention. The protein sequence provided by the invention has the following advantages that the protein sequence has an excellent inhibition function in various tumor cells of bladder cancer, lung cancer and the like; the cancer inhibition effect is quick and obvious; and besides, the sequence provided by the invention is a human-derived sequence, does notcause rejection reaction, and has a wide application prospect.
Owner:广州研展科技有限公司

Neuropeptide and synthesis method and application thereof

ActiveCN106831948AImproving deficits in social memoryChange activity and stateNervous disorderPeptide/protein ingredientsNervous systemNeuron
The invention discloses a neuropeptide and a synthesis method and application thereof, wherein the neuropeptide has an amino acid sequence shown by SEQIDNO:1. According to the amino acid sequence represented by the SEQIDNO:1, valine is used as a first amino acid,-NH2 of the valine is exposed, then the-NH2 of the valine is condensed with-COOH of second amino acid-arginine to form an amide bond, other amino acids are sequentially connected, and finally tyrosine is to obtain the neuropeptide. Compared with the prior art, the neuropeptide has the following advantages: 1. the neuropeptide product can effectively improve defects of experimental animal social memory, and no adverse reaction is observed; 2. functional areas of the neuropeptide is a sequence on an endogenous protein, and cell rejection reaction may not be caused; 3. a signaling pathway activated by the neuropeptide is a known nervous system specific signaling pathway; 4. the product can effectively change the activity and state of neurons; and 5. the essence of the product is an amino acid sequence, is easy to degrade, and may not cause bodily organ loads.
Owner:SOUTHEAST UNIV

3D printed artificial periosteum, artificial bone, artificial skeleton and preparation method thereof

ActiveCN112546299APromote early mineralizationSupports new bone formationAdditive manufacturing apparatusTissue regenerationBone defectComputer printing
The invention discloses a 3D printed artificial periosteum, an artificial bone, an artificial skeleton and a preparation method thereof. The 3D printed artificial periosteum is prepared by compoundingcalcium alginate and nanoscale pig cross-linked collagen fibers as materials through 3D printing according to the shape of the periosteum required by a bone defect part of a patient, wherein the massratio of the calcium alginate to the nanoscale pig cross-linked collagen fibers is 1:(1-5). The 3D printed artificial bone is formed by printing an artificial bone material by using a 3D printer according to the shape of the bone defect part of the patient; and the artificial bone material is prepared from the following components: 1wt%-6wt% of nanoscale pig cross-linked collagen fibers, 3wt%-4wt% of nanoscale hydroxyapatite and 90wt%-96wt% of a degradable biological carrier material. The 3D printed artificial skeleton is composed of the 3D printed artificial bone body and the 3D printed artificial periosteum.
Owner:中国人民解放军总医院第八医学中心

Tissue engineered esophagus

The invention discloses a tissue engineered esophagus and a preparation method thereof. The tissue engineered esophagus contains a dual-layer structure of an epithelial layer and a dermis layer constructed by a scaffold material and seed cells, wherein the scaffold material is acellular matrix, and the seed cells include fibroblasts, vascular endothelial cells, and keratinocytes. The vascular endothelial cells in the dermis layer directly take part in the creation of capillary network, and two revascularization methods are used for quickly communicating with the blood supply channel of receptor, thus the epithele of the tissue engineered esophagus is provided with blood.
Owner:XIAN REJE BIOLOGICAL TECH

Blood glucose regulating polymer vesicle as well as preparation method and application thereof

The invention relates to a blood glucose regulating polymer vesicle which can be applied to treating diabetes as well as a preparation method and application thereof. The polymer vesicle is prepared from amphipathic glycopolymer. The preparation method of the blood glucose regulating polymer vesicle comprises the following steps: firstly, preparing the amphipathic glycopolymer at least from an initiator, a chain transfer agent and a polymer monomer; then enable the amphipathic glycopolymer to conduct self-assembling to form the blood glucose regulating polymer vesicle. The blood glucose regulating polymer vesicle disclosed by the invention can respond to concentration change of glucose in the external environment to generate particle diameter change, cannot be affected by temperature, further has better stability and can be used as a blood glucose detection agent or a non-drug buffer agent for treating the diabetes.
Owner:TONGJI UNIV

Obstructive nephroma animal model and its manufacturing method

In the method, the corrosion resisting metal clamp is set on ureters at two sides of animal living body model. The said clamp made from metal wire or sheet material in U type structure composed of two handles and one circular arc, which clamps on ureters. The invention possesses the advantages of favorable histocompatibility, rest interior of the body for a long time without reject reaction, simple modeling procedure and good repeatability.
Owner:GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE

Degradable and foldable biological amnion composite repair stent

The invention discloses a degradable and foldable biological amnion composite repair stent. The degradable and foldable biological amnion composite repair stent comprises a tube-shaped body with an axially extending through hole, an elastic balloon is arranged at the front end of the tube-shaped body, a one-way valve is connected to the tail end of the tube-shaped body, the one-way valve closes the through hole at the position, a foldable net-pipe-shaped polylactic acid bracket covers the outer surface of the elastic balloon, a biological amnion covers the outer surface of the foldable net-pipe-shaped polylactic acid bracket, a plurality of tiny holes are formed in net filaments of the foldable net-pipe-shaped polylactic acid bracket, and biological amnion powder fills the plurality of tiny holes; under the initial state, the elastic balloon, the foldable net-pipe-shaped polylactic acid bracket and the biological amnion are compressed to in the compact state; and under the use state, after the stent is implanted into the body and swelling occurs due to pressurizing, the tube-shaped or waterdrop-similar-shaped state can be formed complying with the lacrimal duct / uterine cavity, or the other spatial states adaptive to body cavity also can be formed.
Owner:JIANGXI RUIJI BIOTECH CO LTD

A kind of blood sugar regulation polymer vesicle and its preparation method and application

The present invention relates to a blood sugar regulating polymer vesicle which can be used for diabetes treatment and its preparation method and application. The polymer vesicle is assembled by amphiphilic sugar-containing polymer; transfer agent and polymerized monomers to synthesize amphiphilic sugar-containing polymers, and then self-assemble the amphiphilic sugar-containing polymers to form blood sugar-regulating polymer vesicles; the blood sugar-regulating polymer vesicles of the present invention can respond to glucose in the external environment The particle size changes with the change of concentration, it is not affected by temperature and has good stability, and can be used as a blood sugar detection reagent and a non-drug buffer for treating diabetes.
Owner:TONGJI UNIV
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