The invention discloses a thermosensitive microcarrier as well as a preparation technology and an application method thereof. The thermosensitive microcarrier can be used for amplifying, culturing and harvesting high-quality and high-activity cells used for clinical treatment, and the high-quality and high-activity cells are applied to the fields of cell therapy and tissue engineering. The preparation technology comprises the following steps of: grafting active groups by virtue of chemical surface treatment while a high polymer material cell culture microballoon sphere as a matrix, then carrying out reaction on the high polymer material cell culture microballoon sphere and thermosensitive polymer to graft the thermosensitive polymer, so as to obtain a thermosensitive microcarrier used for cell culture, and controlling the temperature during cell culture, so that cells grow and are amplified while being attached onto the surface of the thermosensitive microcarrier; then lowering temperature and haversting cells with complete surface protein. On one hand, a method for amplifying massive cells used for treatment in a short time, on the other hand, no pancreatin or traditional dissociative reagent is used, so that damage on surface protein and ion channel of cells is reduced, the original function of the cells is maintained, and the thermosensitive microcarrier has strong clinical application value and economic value.