106 results about "Cardiac muscle tissue" patented technology

Coronary sinus ring apparatus, including an elongated body for deployment in a coronary sinus, an aperture in a long side of the body, at least a partial lumen extending from one end of the elongated body to at least the aperture, and a guide within the elongated body positioned and shaped to guide an element inserted along the lumen to the aperture. Apparatus for treating a heart, including a delivery tube sized for and adapted for insertion into a body, a sharp tip adapted to be pushed through cardiac muscle, an elongate tensioning element, and a foldable anchor adapted to couple the elongate tension element to cardiac muscle tissue, wherein the delivery tube encloses one or both of the elongate tensioning element and the foldable anchor. Related apparatus is also described.

The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and / or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and / or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

The invention relates to a dual-purpose lasso catheter with irrigation. Cardiac catheters, including a lasso catheter, are provided for use in a system for electrical mapping of the heart has an array of raised, perforated electrodes, which are in fluid communication with an irrigating lumen. There are position sensors on a distal loop section and on a proximal base section of the catheter. The electrodes are sensing electrodes that may be adapted for pacing or ablation. The raised electrodes securely contact cardiac tissue, forming electrical connections having little resistance.

The invention belongs to the field of researching and application of biomedicine and particularly relates to human pluripotent stem cell-hereditary cardiomyopathy cardiac muscle cells and a preparation method thereof. The invention provides a human hereditary cardiomyopathy-pluripotent stem cell, which is constructed by means of TALEN or CRISPR / CAS9 genome editing technology. The human hereditary cardiomyopathy cardiac muscle cell can be combined with any scaffold materials to culture various in-vitro human hereditary cardiomyopathy cardiac muscle tissues. The human hereditary cardiomyopathy cardiac muscle cells in the invention have disease phenotypes and electrophysiology change being similar as the cardiac muscle cells of human hereditary cardiomyopathy patients. The human hereditary cardiomyopathy cardiac muscle cells are wide in sources and can be cultured in-vitro for a long time. The invention provides excellent tools and platforms for researching an effective new therapy means and researching an effective corresponding treatment medicine.

The ablation of tissue in a cardiac wall is performed by introducing an endocardial catheter into the interior of a heart and an epicardial catheter is placed on the cardiac wall. With the aid of an electromagnet and a counter-element, which can be permanently magnetized or can also be an electromagnet, the two catheters are mutually attracted in such a way that electrodes come into contact with opposite sides of the cardiac wall such that the electrodes are optimally positioned. The use of a catheter with a plurality of such electrodes in a row enables the removal of whole lines of destroyed cardiac wall tissue by ablation.

The invention discloses a non-invasive arrhythmia treatment system, which comprises an imaging device, a processing device and a gathered energy transmission device. The imaging device is used for imaging an object to be treated to construct three-dimensional heart and body models of the object to be treated. The processing device comprises a body surface electric signal acquisition module, an electric activity computation module and an arrhythmia determination module. The body surface electric signal acquisition module is used for acquiring body surface electric signals detected by a plurality of electrodes which are placed at a plurality of positions on a body surface. The electric activity computation module is used for computing electric activities in the internal three-dimensional space of the heart through an inverse problem based on the acquired body surface electric signals and the three-dimensional heart and body models. The arrhythmia determination module is used for determining at least one origin position of arrhythmia at least based on the electric activities in the internal three-dimensional space of the heart obtained through computation. The gathered energy transmission device is used for transmitting gathered energy to the determined origin position to ablate cardiac muscle tissues at the origin position.

The invention relates to an acute myocardial infarction marker and an application thereof, and in particular relates to RFX2 and an application of an agonist thereof in preparing a medicine for diagnosis and treatment on acute myocardial infarction. Based on a high-flux sequencing result, the inventor screens out the RFX2 gene; a molecular biology method verifies that RFX2 has low expression in the peripheral blood and the cardiac muscle tissue of the acute myocardial infarction. The invention provides the potential diagnosis and treatment target spot of the acute myocardial infarction, and has great clinical application values.

This invention provides a catheter used for blocking abnormal conduction in the cardiac muscle using photodynamic therapy or treating arrhythmia and a method for evaluating the therapeutic effects of the catheter. The catheter has a structure that freely bends at its end, which is used for performing photodynamic ablation of cardiac muscle tissue via photochemical reactions in the blood vessel or cardiac lumen. The catheter comprises a light-emitting window for applying a light beam transmitted through an optical fiber to a target site of cardiac muscle tissue and at least two electrodes for potential measurement in the periphery of the light-emitting window.

The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and / or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and / or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue.

The invention discloses a use of a derived polypeptide series of pigment epithelium derived factors in ischemic myocardium protection. The polypeptide is derived from the pigment epithelium derived factors, and every amino acid sequence in the polypeptide includes at least eighteen continuous amino acid residues. The PEDF derived functional peptide has good function advantages, and has remarkablecardiac muscle tissue capillary tube stabilization, vascular leakage prevention, myocardial cell protection and cardiac muscle tissue inflammation prevention effects.

The invention is directed to a method for the preparation of a multiring engineered heart tissue construct suitable for use in cardiac tissue augmentation and / or replacement therapy. The invention further refers to multiring EHT constructs which comprise at least two force-generating engineered heart tissue rings fused with each other and a device for preparing the same. Finally, the invention relates to force-generating engineered heart tissue rings derived from human cells and their use in drug screening and target validation assays.

The invention designs an image tracking method based on multi-neighborhood-aided two-dimensional ultrasonic deformed microstructure, which comprises the steps as follows: a two-dimensional ultrasonic cardiogram of a tested heart object is collected under a high frame frequency condition and used for offline analysis firstly; then any visible image is used as a selected initial frame of a region of interest (ROI) so that the ROI includes cardiac muscular tissues, simultaneously the interferences of grey scale signals is eliminated; after the ROI of the initial frame on an initial image I is completely selected, the original ROI is set as a center; moreover, the ROI and eight areas coating the surrounding of the original ROI and having same sizes are used as a new searching source region, the tracking vectors of all subregions with the sizes the same as the size of the original ROI in the searching area are traversed through an SAD (Sum of Absolute Differences) arithmetic; and weighted average is implemented on each tracking vector finally, thus obtaining the tracking result of the original ROI. Compared with the traditional SAD tracking method, the neighborhood tracking result of the ROI is sufficiently considered; therefore, the image tracking method based on multi-neighborhood-aided two-dimensional ultrasonic deformed microstructure has excellent noise immunity, and is especially beneficial for movement estimation and parameter calculation on movement issues.

The invention discloses a two-photon fluorescence probe for detecting peroxynitrite as well as a preparation method and application of the two-photon fluorescence probe. The two-photon fluorescence probe has the structural formula as shown in the description. The two-photon fluorescence probe is named as TPNIR-FP. The two-photon fluorescence probe adopts a Nile red derivative as a fluorescent group and an alpha-keto-amide functional group as an ONOO-reaction group, can rapidly respond to ONOO- (within 10 seconds), and in addition is high in sensitivity and good in selectivity. The two-photon fluorescence probe can be applied to imaging research on ONOO- in antharcycline antibiotic induced cardiac muscle cell and cardiac muscle tissue damage.

A non-invasive method of producing a three-dimensional cardiac electrogram characteristic map for use in catheter ablation of ventricular tachycardia includes receiving left ventricle three-dimensional image data of a patient's heart; segmenting a left ventricle image of the patient's heart based on the left ventricle three-dimensional image data into scar tissue, normal myocardium tissue and left ventricle cavity regions; determining scar tissue thickness and normal myocardium tissue thickness for a plurality of portions of the left ventricle image of the patient's heart; receiving predetermined data that associate a value of at least one electrogram characteristic to each scar tissue thickness and each normal myocardium tissue thickness for the plurality of portions of said left ventricle image of said patient's heart; and generating the three-dimensional cardiac electrogram characteristic map of the at least one electrogram characteristic corresponding to the left ventricle image of the patient's heart based on the predetermined data.

[Summary][Object]To develop a preparation and a treatment method effective for improving a state of a bald hear, promoting hair growth, and restoring injuredskin tissues, supporting tissues, and cardiac muscle.[Solution]A preparation containing atrial diuretic hormone family molecules are used for restoration and regeneration of a tissue and an organ. The preparation is used for growing hairs and / or for improving and restoring failures of skin, mucosa, and subcutaneous tissue such as bed bore, rough and dry skin, burn injuries, necrosis, and psoriasis, and also for improving cardiac muscle failures.

The invention belongs to the field of regenerative medicine of cardiac muscle tissues, and discloses application of ascorbic acid as an induction factor in inducting differentiation of iPS (induced pluripotent stem) cells into cardiac muscle cells and a differentiation-inducing culture medium containing ascorbic acid. The invention also discloses a method for inducing differentiation of iPS cells into cardiac muscle cells by using the differentiation-inducing culture medium, which comprises the following step: inducing an iPS cell derived embryoid body, which has grown in a suspension mode for 5-7 days, to differentiate by utilizing the differentiation-inducing culture medium, thereby obtaining cardiac muscle cells. By using ascorbic acid as the induction factor, the invention obviously enhances the differentiation efficiency of the iPS cells into the cardiac muscle cells, and the differentiation percentage can reach 50-60%; the differentiation-inducing culture medium does not have toxic or side effect on the cells; and in addition, the method provided by the invention is simple, has the advantage of low cost, can be operated in common laboratories, and is suitable for large-scale proceeding.

The invention belongs to the field of biomedical engineering, and relates to a human tissue-engineered cardiac muscle tissue and a preparation method thereof. The human tissue-engineered cardiac muscle tissue disclosed by the invention is prepared by inoculating all kinds of cell components of a normal heart differentiated from human induced multipotential stem cells by taking a natural decellularized heart substrate as the scaffold. The human tissue-engineered cardiac muscle tissue has cell components, extracellular matrixes and biological functions similar to that of a normal human cardiac muscle tissue; testing and screening of in-vitro drugs, initial in-vivo transplantation researches of clinical earlier-stage small animals and observation of treatment effects can be carried out; and helps can be finally offered for realizing individualized treatment of human cardiovascular diseases.

The invention discloses a cardiac muscle tissue engineering product and its preparation method and use. The preparation method comprises 1, omentum majus decellularized matrix scaffold material preparation: immersing omentum majus in a decellularizing liquid containing lauryl sodium sulfate, DNA enzyme and trypsin so that decellularizing treatment is realized and 2, myocardial cell-omentum majus decellularized matrix complex construction and culture. The cardiac muscle tissue engineering product comprises the myocardial cell and omentum majus decellularized matrix scaffold material. The omentum majus decellularized matrix has a good 3D netted space structure and is rich in a growth factor so that myocardial cell growth and nutrient and metabolite transport are realized. The cardiac muscle tissue engineering product has an important meaning for cardiac muscle damage restoration based on the cardiac muscle tissue engineering product and cardiac muscle treatment drug screening utilizing the cardiac muscle tissue engineering product as a model.

The invention discloses a chip device for three-dimensional cell culture and in-situ real-time monitoring of cardiac muscle tissues and belongs to the technical field of organ chips. The chip device comprises a PDMS (Polydimethylsiloxane) substrate, wherein the PDMS substrate is provided with two cell culture cavities for culturing cardiac muscle cells at interval; biological electrodes are respectively and correspondingly arranged in the cell culture cavities, and the two cell culture cavities are communicated; the biological electrodes connect chips and external electric stimulation device or electric-signal acquisition device respectively by inputting or outputting of electric signals, and carry out electric stimulation on the cardiac muscle tissues or real-time acquisition on electricsignals of cardiac muscle tissues. The chip device disclosed by the invention has the beneficial effects that the cardiac muscle cells are inoculated in the culture cavities and can grow and be anchored on two electrode masts to realize in-vitro three-dimensional culture of the cardiac muscle cells, so that the two electrodes are utilized for electrophysiological-signal acquisition and electrical-stimulation experiment and the like, and further the applications of drug screening and environmental-poison evaluation and the like can be carried out.

The invention discloses a low-temperature ablation catheter, a low-temperature ablation operating device and a low-temperature ablation device, and belongs to the technical field of the low-temperature ablation. The low-temperature ablation catheter comprises a first capsule, a core pipe and a thermal-insulating part. The first capsule is installed at the front end of the core pipe. The first capsule is provided with a front end area suitable for attaching a cardiac muscle tissue in a low-temperature ablation process and a rear end area exposed in blood. The core pipe is internally provided with a first loop. The first loop is suitable for charging low-temperature first fluid into the first capsule or flowing out the low-temperature first fluid. The thermal-insulating part is at least partially attached with the rear end area, and is suitable for reducing the heat exchange efficiency between the first fluid and the blood in atriums. The provided technical scheme is capable of effectively reducing the heat exchange between the low-temperature ablation catheter and the blood in the low-temperature ablation process.

The invention provides a method for preparing a cardiac muscle tissue engineering decellularization collagen diaphragm. The method comprises the following step: selecting natural pork tendon tissue, and performing decellularization on the tissue by using treatment methods of repeated freezing and thawing and nuclease treatment, thereby obtaining decellularization pork tendon diaphragms of different thicknesses, wherein the thicknesses of the diaphragms are within 5-30[mu] m; the obtained diaphragms comprise collagen as a main component; and the diaphragms are of structures of fibers which are arranged in parallel, are similar to extracellular matrix components and structures of cardiac muscle tissue cells, can induce cardiac muscle cells to extend along the fiber direction, and thus can be used for culturing cardiac muscle cells and establishing heart-like tissue. The method has the advantages of being convenient in material obtaining, low in price, stable in preparation process, uniform in product morphology, and simple and gentle, and collagen diaphragms can be highly controlled, have complete structures, morphology, biological properties and inherent mechanical properties of materials, furthermore have good biocompatibility and biodegradability, and are very applicable to cardiac muscle tissue engineering construction and regenerative medicine.

The invention discloses a dual-pole cardiac muscle tissue one-way action potential recording electrode. The dual-pole cardiac muscle tissue one-way action potential recording electrode comprises a puncture needle rod, lower endocardium cardiac muscle recording electrode installation holes and middle layer cardiac muscle recording electrode installation holes are formed in the outer wall of the puncture needle rod, a pair of lower endocardium cardiac muscle recording electrode wires and a pair of middle layer cardiac muscle recording electrode wires are arranged in an inner cavity of the puncture needle rod, the front ends of the lower endocardium cardiac muscle recording electrode wires are fixed to the lower endocardium cardiac muscle recording electrode installation holes, the front ends of the middle layer cardiac muscle recording electrode wires are fixed to the middle layer cardiac muscle recording electrode installation holes, and the back ends of the lower endocardium cardiac muscle recording electrode wires and the back ends of the middle layer cardiac muscle recording electrode wires are connected with one ends of four transmission leads; a rigid positioning sleeve is arranged on the outer wall of the puncture needle rod, lower epicardium cardiac muscle recording electrode installation holes are formed in the rigid positioning sleeve, a pair of lower epicardium cardiac muscle recording electrode wires are arranged in the lower epicardium cardiac muscle recording electrode installation holes, the front ends of the lower epicardium cardiac muscle recording electrode wires are formed in the lower epicardium cardiac muscle recording electrode installation holes, and the back ends of the lower epicardium cardiac muscle recording electrode wires are connected with one ends of another two transmission leads. The one-way action potential of three layers of myocardial cells can be recoded at the same time, and the dual-pole cardiac muscle tissue one-way action potential recording electrode is ingenious in structural design, and easy and convenient to use.

The invention discloses a marker used for assisted detection of abrupt highland advancing low pressure low oxygen environment related myocardial damages, and applications of a product used for detection of miR-144-3p expression level in preparation of kits used for auxiliary diagnosis of myocardial damages caused by low pressure low oxygen. According to the applications, a low-pressure oxygen cabin is adopted for stimulation of highland low pressure low oxygen environment, and establishment of rat animal models with low pressure low oxygen myocardial damages. High flux sequencing technology and bioinformatics methods are adopted, key miRNA related to highland low pressure low oxygen myocardial damages are screened, and fluorogenic quantitative PCR is adopted for experiment verifying of thekey miRNAs in cardiac muscle tissues and blood plasma. The miRNAs disclosed in the invention can be used for assisted detection of abrupt highland advancing low pressure low oxygen environment related myocardial damages, and possess important meaning in diagnosis and assessment of abrupt highland advancing low pressure low oxygen environment related myocardial damages.

Provided is a shock wave focusing device for a shock wave ablation system for coagulating and necrosing cardiac muscle tissue that becomes a cause of arrhythmia. A shock wave generation apparatus (10) comprises: a shock wave focusing device (11) which has a concave surface (11a); an optical fiber (12) which is inserted into the shock wave focusing device (11); a tubular catheter (13) which guides the optical fiber; an enclosure (14) which constitutes a space to be filled with liquid at the tip of the optical fiber (12); and the liquid (L) which is filled into the enclosure (14). The shock wave focusing device (11) is configured from a ring-shaped coupling part (16) provided with a central hole (11b), and 16 blade parts (17) curved outward from the edge of the coupling part (16) toward the front and provided elastically to the edge of the coupling part (16), and can be folded by rotating the blade parts (17) with respect to the coupling part (16).

The invention discloses application of EMD638683 in protection of the heart suffering from acute myocardial infarction. The invention provides application of EMD638683 in preparation of products having the following functions: 1) prevention and / or treatment of myocardial infarction of animals; 2) prevention and / or treatment of heart failure caused by myocardial infarction of animals; and 3) inhibition of the expression of SGK1 protein in cardiac muscle tissue after myocardial infarction of animals. Experimental results of the invention prove that EMD638683 has treatment effect on mice with myocardial infarction, and experimental data is provided for clinical application of EMD638683 in treatment of myocardial infarction. The EMD638683 is of important theoretical significance to research on and development of medicines used for preventing and treating heart failure caused by myocardial infarction and has good application prospects.

The invention relates to a traditional Chinese medicine formula, in particular to a traditional Chinese medicine formula for treating heart qi deficiency and aims to solve the technical problems that the existing medicine for treating the heart qi deficiency has a great side effect, cures the symptoms not the disease, is not scientific in compatibility and is bad in treatment effect. The traditional Chinese medicine formula for treating the heart qi deficiency comprises the following raw materials in parts by weight: 8-12 parts of white ginseng, 8-12 parts of cassia twig, 13-17 parts of mongolian snakegourd, 13-17 parts of allium macrostemon, 8-12 parts of rhizoma pinelliae preparata, 13-17 parts of root of common peony, 13-17 parts of radix ophiopogonis and 3-7 parts of coptis chinensis. The traditional Chinese medicine formula for treating the heart qi deficiency, disclosed by the invention, can effectively improve the cardiac function of a model rat suffering from the heart qi deficiency, promotes recovery of scathing cardiac muscle tissues, adjusts biochemical criterion, and has an excellent treatment effect on the model rat suffering from the heart qi deficiency; as proved by clinical trial, the traditional Chinese medicine formula has a total effective rate of more than 90% in treating the heart qi deficiency, is free from toxic and side effects, and is used in the field of treating heart qi deficiency syndrome.

The invention provides an integrated anchor which comprises a locking rod, a locking sleeve arranged outside the locking rod in a sleeving mode and an anchoring piece. The anchoring piece is connected with the far end of the locking rod or the far end of the locking sleeve; a radial gap is formed between the locking rod and the locking sleeve; the locking rod is provided with a threading hole for threading artificial chordae tendineae; at least one end of the threading hole is communicated with the radial gap between the locking rod and the locking sleeve; the locking rod and the locking sleeve move relatively, so that the artificial chordae tendineae is locked in the radial gap between the locking rod and the locking sleeve; and the anchoring piece is used for being anchored into cardiac muscle tissues. The integrated anchor provided by the invention integrates the functions of anchoring and locking the artificial chordae tendineae, and when the integrated anchor is applied to a chordae tendineae repair operation, the surgical operation can be simplified, the number of instruments is reduced, and the cost is reduced.

The invention relates to an exosome derived from a mesenchymal stem cell. The exosome is prepared by intermittent hypoxic stimulation of the mesenchymal stem cell. The exosome prepared from the invention has stronger tissue and organ ischemia-reperfusion injury protective effect, and the biological effect of unit exosomes is improved, and treatment of diseases such as ischemic heart disease is more facilitated.

Coronary sinus ring apparatus, including an elongated body for deployment in a coronary sinus, an aperture in a long side of the body, at least a partial lumen extending from one end of the elongated body to at least the aperture, and a guide within the elongated body positioned and shaped to guide an element inserted along the lumen to the aperture. Apparatus for treating a heart, including a delivery tube sized for and adapted for insertion into a body, a sharp tip adapted to be pushed through cardiac muscle, an elongate tensioning element, and a foldable anchor adapted to couple the elongate tension element to cardiac muscle tissue, wherein the delivery tube encloses one or both of the elongate tensioning element and the foldable anchor. Related apparatus is also described.