The invention relates to a preparation method and a storage method for
placenta-derived
mesenchymal stem cell exosome, and belongs to the technical field of stem cells and regenerative medicines. Thepreparation method comprises the following steps of: culturing a P3 generation
placenta-derived
mesenchymal stem cell; after a degrees of fusion reaches 80-90%, sucking the P3 generation
placenta-derived
mesenchymal stem cell out of a culture medium, and culturing in a DMEM (Dulbecco's modified
eagle medium) for 24 h; then, collecting liquid supernatant, and removing
cell debris; filtering througha 0.22 [mu] m
sterile filtration membrane, taking out a filtrate, carrying out
centrifugation at a revolving speed of 13000 r / min for 60 min, filtering the liquid supernatant through the 0.22 [mu] msterile
filtration membrane to obtain high-purity
exosome, and finally, carrying out re-suspension by normal
saline and
centrifugation at a revolving speed of 13000 r / min for 65 min to obtain the concentrated
exosome; and adding the exosome into a
cryopreservation liquid containing an SR serum-
free medium,
glycerol and
vitamin C, freezing at a temperature of 80 DEG C below zero for 24h, taking out, and carrying out
cryopreservation in
liquid nitrogen at a temperature of 196 DEG C below zero. The above preparation method for the placenta-derived mesenchymal
stem cell exosome aims at deficiencies in the conventional art, an extraction method is regulated, and the
cryopreservation liquid is improved to obtain the high-purity high-density exosome capable of keeping activity.