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Mesenchymal stem cell exosome preparation and application thereof

A technology of stem cells and exosomes, applied in the field of stem cells, can solve the problems of few reports on the application of exosomes of umbilical cord mesenchymal stem cells, and achieve the effects of less side effects, a wide range of material sources, and reducing lipid accumulation.

Inactive Publication Date: 2020-02-11
上海奈康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the application of exosomes from umbilical cord mesenchymal stem cells.

Method used

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  • Mesenchymal stem cell exosome preparation and application thereof
  • Mesenchymal stem cell exosome preparation and application thereof
  • Mesenchymal stem cell exosome preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1: A kind of mesenchymal stem cell exosome preparation:

[0069] This embodiment provides a mesenchymal stem cell exosome preparation, which specifically includes:

[0070] Exosomes derived from mesenchymal stem cells and curcumin monophosphate inclusion complex with a mass ratio of 1:0.2, and a pharmaceutically acceptable carrier;

[0071] Wherein, the inclusion compound of curcumin monophosphate salt is prepared by inclusion of the HP-β-CD mixture with DS=4.5 and DS=7.8 as the main body and curcumin monophosphate monopotassium salt as the guest of clathrates.

[0072] The exosome preparation is in the form of injection.

[0073] The pharmaceutically acceptable carrier is physiological saline.

[0074] Relative to the total volume of the exosome preparation, the exosome content in the exosome preparation is 1000 μg / mL.

[0075] The mesenchymal stem cells are selected from bone marrow mesenchymal stem cells, and the isolation and culture process adopts ...

Embodiment 2

[0098] Example 2: Another kind of mesenchymal stem cell exosome preparation:

[0099] This example provides another mesenchymal stem cell exosome preparation. The method and materials used in this example are basically the same as those in Example 1, except that the exosome preparation in this example does not contain curcumin monophosphate Salt inclusion complex.

Embodiment 3

[0100] Example 3: Another kind of mesenchymal stem cell exosome preparation:

[0101] This example provides another mesenchymal stem cell exosome preparation. The method and materials used in this example are basically the same as those in Example 1, except that when preparing the curcumin monophosphate inclusion complex in this example , select HP-β-CD with DS=4.5 as the main body.

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Abstract

The invention belongs to the technical field of stem cells, and particularly discloses to an umbilical cord mesenchymal stem cell exosome preparation and application thereof. The umbilical cord mesenchymal stem cell exosome preparation specifically includes exosomes derived from mesenchymal stem cells and a curcumin monophosphate ester salt inclusion compound with the mass ratio being 1:(0.15 to 0.25) and a pharmacologically acceptable carrier, wherein the curcumin monophosphate ester salt inclusion compound is prepared by inclusion with a hydroxypropyl-beta-cyclodextrin mixture with differentdegrees of substitution as a main body and a curcumin monophosphate ester salt as an object. According to the preparation, the exosomes have high purity and content, no precipitation is precipitatedduring preparing of the curcumin monophosphate ester salt inclusion compound, the inclusion rate is high, the preparation can significantly reduce lipid accumulation of Chang liver cells, thus a purpose of reducing fat is realized, side effects are avoided or side effects are small, and adverse reactions to a user cannot be caused.

Description

technical field [0001] The present invention relates to the technical field of stem cells, in particular to a mesenchymal stem cell exosome preparation and its application. Background technique [0002] Exosomes refer to small membrane vesicles (30-150nm) that contain complex RNA and proteins. Nowadays, they specifically refer to discoid vesicles with a diameter of 40-100nm. In 1983, exosomes were first discovered in sheep reticulocytes, and Johnstone named them "exosomes" in 1987. A variety of cells can secrete exosomes under normal and pathological conditions. It is mainly derived from the multivesicular body formed by the invagination of lysosomal particles in the cell, and is released into the extracellular matrix after the fusion of the outer membrane of the multivesicle and the cell membrane. All cultured cell types secrete exosomes, and exosomes are naturally present in bodily fluids, including blood, saliva, urine, cerebrospinal fluid, and breast milk. The precise...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K31/661A61K47/69A61P3/04A61P3/06A23L33/00A23L33/10
CPCA23V2002/00A61K31/661A61K35/28A23L33/00A23L33/10A61K47/6951A61P3/04A61P3/06A61K2300/00A23V2200/332
Inventor 陈相波雷鸣田朋飞应荣
Owner 上海奈康生物科技有限公司
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