The invention relates to the technical field of anti-caries functional dressings, particularly to a medical biological fluorine anti-caries dressing and a preparation method thereof. The medical biological fluorine anti-caries dressing is composed of modified chitosan, sorbitol, glycerol, silica, sodium fluoride, aluminium hydroxide, zinc citrate, peppermint oil, strontium acetate, a garlic extracting solution and carbomer. The medical biological fluorine anti-caries dressing can prevent caries, remove scales on the surfaces of teeth, maintain cleaning of an oral cavity, can effectively prevent gingivitis, periodontitis and bleeding gums and also has the efficacies of desensitization, analgesia, ozostomia removal and the like, so that the medical biological fluorine anti-caries dressing comprehensively maintains health of teeth and the oral cavity and has the characteristics of high safety, non toxic or side effect, and good convenience in use.