334 results about "Near ir fluorescence" patented technology

The invention belongs to the technical field of fluorescent materials, particularly relates to near-infrared fluorescent powder, and further discloses a preparation method of the fluorescent powder, and a light-emitting device containing the fluorescent powder. The near-infrared fluorescent powder comprises an inorganic compound having a composition formula of AxRrQqZy:zD, an excitation wavelengthof the compound is 200-700 nm, and the compound has broadband emission with an emission main peak of 700-1600 nm in a near-infrared light region. The near-infrared fluorescent powder provided by theinvention has a wider excitation wavelength, can well absorb ultraviolet light, blue light and red light, and has stronger near-infrared luminescence than gallium germanate system near-infrared fluorescent powder without Gd.

The present invention discloses Near Infrared (NIR) fluorescent albumin nanoparticles having a structure selected from a core structure or a core-shell structure. Also disclosed are a process of preparing these NIR fluorescent albumin nanoparticles, and a method of in vivo detection of pathologies, in particular cancer pathology, by using administering these NIR fluorescent albumin nanoparticles to a patient.

The invention discloses a near-infrared fluorescent magnetic micro-emulsion nanometer particle, its preparation method and application in tumor treatment, wherein magnetic nano-particles and near-infrared luminescent quantum dot nano-particles or near-infrared luminescent organic dyestuff molecules are embedded into water-in-oil micro-emulsion, anti-cancer drugs can also be embedded into the micro-emulsion, the magnetic steering action of the magnetic nano particles will target the micro-emulsion embedded near-infrared fluorescent substances to tumor positions or secure them onto tumor positions under the excitation of near-infrared lights, the thermal effects produced by the near-infrared lights can kill tumor cells, the OH and O2 free radicals with high activity and the optical catalytic activity of the quantum dots can also have synergic actions in destroying tumor cells.

The invention discloses a synthesis method for a near-infrared fluorescent probe copper nano-cluster and a detection method for glycoprotein. The near-infrared fluorescent probe copper nano-cluster is synthesized through a one-pot method, aminophenylboronic acid is used for functionally transforming the synthesized copper nano-cluster, and the functionalized copper nano-cluster is obtained. Due to the specific action of boric acid groups on aminophenylboronic acid and cis diol in glycoprotein, the fluorescence intensity of the copper nano-cluster is quenched. A fluorescent probe can detect glycoprotein at high specificity and sensitivity. Glycoprotein widely exists in mucus, plasma, cytoplasm and cell membranes, is tightly related to living organisms, is an important physiological active material, and is tightly related to certain genetic diseases, and therefore important practical significance is provided for detecting glycoprotein.

A series of conjugated polymer-based nanoparticles having far-red / near infrared emission ranges are disclosed. Cross coupling methods to prepare the conjugated polymers and methods of nanoparticle preparation are also discussed. The conjugated polymer nanoparticles are used as FR / NIR fluorescent probes in in vitro and in vivo biosensing and bioimaging applications, and are also used in photoacoustic imaging as contrast agents. Finally, use of the conjugated polymer nanoparticles in photothermal therapy is described.

The invention provides a family of fluorescent compounds. The compounds are substituted silaxanthenium compounds that can be chemically linked to one or more biomolecules, such as a protein, nucleic acid, and therapeutic small molecule. The compounds can be used for imaging in a variety of medical, biological and diagnostic applications. The dyes are particularly useful for in vitro, in vivo and ex vivo imaging applications.

The invention belongs to the field of small molecular fluorescent probes and particularly relates to a near infrared fluorescent probe for detecting zinc ions in a water phase and a preparation method thereof. The near infrared fluorescent probe for detecting the zinc ions in the water phase is structured in the specification. The fluorescent probe has the advantages of high selectivity and high sensitivity, as well as an emission peak of fluorescence spectrum in a near infrared area, low cytotoxicity, good membrane permeability and good water solubility. The fluorescent probe has a capability of detection of the zinc ions and fluorescence imaging in cells.

The present invention provides particles comprising either a water-soluble polymer or a phospholipid, wherein at least one near infrared (NIR) fluorescent probe and optionally at least one active agent such as a targeting moiety, capable of selectively recognizing a particular cellular marker, are non-covalently bound to the outer surface of the particles. Pharmaceutical compositions comprising these particles may be used, inter alia, for detection and treatment of tumors in the gastrointestinal tract

The invention belongs to the technical field of luminescent materials, particularly relates to near-infrared fluorescent powder, and further discloses a preparation method of the near-infrared fluorescent powder and a luminescent device containing the fluorescent powder, wherein the near-infrared fluorescent powder comprises an inorganic compound with a composition formula of AxRpOr, an AxRpOr structure is used as a matrix and is doped with specific rare earth ions or transition metal ions to form the compound, the excitation peak of the prepared fluorescent powder is located at 350-750 nm, broadband emission is shown under the excitation of ultraviolet light, blue light or red light, and the emission main peak is located at 700-1600 nm. According to the invention, the near-infrared fluorescent powder materials with different emission wavelengths can be prepared by constructing solid solutions through ion substitution and other methods due to the influence of crystal field splitting, so that the luminous intensity of the fluorescent powder is remarkably improved, the spectrum adjustability is realized, and the application range of the fluorescent powder material is further expanded.

The invention discloses a matrix metalloproteinase-2 specific multi-modality molecular image probe preparation method and application thereof. The preparation method comprises the following steps: 1)preparing peptide substrates for matrix metalloproteinase-2(MMP-2) specific identification; 2) modifying near infrared fluorescent dye on peptide substrate; 3) modifying fluorescent quenching group onpeptide substrate; 4) connecting different molecular weight PEG or tumor targeting group RGD on peptide substrate modified with near infrared fluorescent dye and fluorescent quenching group; 5) labeling the nuclide on the above modified peptide substrate side chain tyrosine. Compared with the prior art, the matrix metalloproteinase-2 specific multi-modality molecular image probe preparation method has the advantages that: 1) by utilizing the specificity and responsiveness of the MMP-2 protease by the probe, so that the probe is selectively enriched at the tumor site so as to improve the targeting property of the probe to the tumor; 2) performing multi-modality imaging in a living body tumor by using advanced molecular imaging technology such as optical, opto-acoustic, SPECT and the like,improving the sensitivity and accuracy of tumor imaging, and finally achieving accurate positioning of the tumor; 3) providing a new train of thought and method for early diagnosis, process research and prognosis evaluation of tumor.

The invention belongs to the technical field of analysis and detection, and provides a lysosome-targeted Cys near-infrared fluorescent probe and a preparation method and application thereof. The near-infrared fluorescent probe is 3-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohexen-1-yl)vinyl)-4-morpholinyl coumarin-7-acrylate which is abbreviated as DCICA. The prepared lysosome-targeted Cys near-infrared fluorescent probe has the characteristics of novel structure, lysosome targeting performance and near-infrared fluorescence emission (wherein an emission wavelength is 680 nm), can realize high-selectivity high-sensitivity detection of a Cys content of a solution, conducts specific detection of Cys in cell lysosome by using a laser confocal imaging technology, is of important guiding significance to research on the molecular mechanism of participation of the Cys in the life activity of the lysosome, and has a wide application prospect.

The invention provides a fluorescent probe with the advantages of reduced background interference, high sample penetration performance, good selectivity, high sensitivity and good imaging resolution for fluorescence open-close type near infrared detection of cysteine as well as a preparation method and application of the fluorescent probe. The fluorescent probe is subjected to fluorescence quenching by taking near infrared fluorescent parent nucleus containing a plurality of double bonds as a fluorophore and taking 2,4-dinitrobenzene sulfonamide as a quenching unit; in actual detection, in thepresence of the cysteine, sulfydryl of the cysteine carries out nucleophilic substitution reaction on electron deficient aromatic ring, a sulfonamide bond cracks and a fluorescence quencher is dissociated, so that the fluorescent probe without fluorescence or with weak fluorescence based on intramolecular charge transfer (ICT) process releases the fluorescent parent nucleus, fluorescence is enhanced or opened to generate a fluorescent signal for selectively recognizing the cysteine, and further the aims of selective recognition and analysis and detection of the cysteine (Cys) are achieved.

The invention belongs to a field of medicinal preparations, and relates to a liposome drug delivery system used for tumor imaging, wherein the system is modified by a polypeptide with an amino acid sequence of RPAKPAR and wraps a near-infrared fluorescent dye. According to the invention, the RPAKPAR polypeptide is a targeting head group and modified on surface of stealth liposome, and wraps the near-infrared fluorescent dye. After intravenous administration, through mediation effect of the RPAKPAR polypeptide, the carrier stealth liposome actively sends the near-infrared fluorescent dye to tumor positions, and penetrates tumor vasculatures into whole tumor tissues, thereby increasing distribution of the dye in the tumor positions; and then by using an in-vivo optical molecular imaging technology, the tumor imaging with relatively high fluorescent signal intensity can be provided.

The invention discloses a leucine aminopeptidase and monoamine oxidase-activated near-infrared fluorescent probe as well as a synthetic method and biological application thereof. The adopted syntheticmethod includes the following step: (1) synthesizing the novel leucine aminopeptidase and monoamine oxidase-activated near-infrared fluorescent probe (NML); and the biological application comprises the following step: (1) applying the probe to biological imaging and serum detection. The leucine aminopeptidase and monoamine oxidase-responsive fluorescent probe is synthesized for the first time, and the problem of low specificity of a traditional single-enzyme fluorescent probe is overcome; the fluorescent probe has near-infrared fluorescence properties, the background fluorescence signal can be effectively reduced, and the sensitivity of the probe can be improved; and the fluorescent probe has a good staining effect on living cells and high staining efficiency, and can detect cell endogenous leucine aminopeptidase and monoamine oxidase.

The invention relates to a kind of ring-fused structural near-infrared photosensitizer and a preparation method thereof, especially relates to a kind of a photosensitizer treating malignant tumor through photodynamic therapy and a preparation method thereof, and concretely provides a kind of a heavy-atom-substituted near-infrared aza-BODIPY-like (aza-boron dipyrromethene-like) photosensitizer, a preparation method thereof, and efficiency of the photosensitizer generating singlet oxygen. The photosensitizer is a series of novel photosensitizers taking heavy-atom-substituted near-infrared fluorescent dye aza-BODIPY developed from azodipyrromethane boron trifluoride as a mother nucleus. The general formula of the phoosensitizer is shown as formulas I / II / III / IV. The aza-BODIPY-like photosensitizer possesses a ring-fused structure, and also possesses advantages of classic traditional dye BODIPY / aza-BODIPOY spectrum performances. The ring-fused structural aza-BODIPY-like photosensitizer has the absorption wavelength more than 700 nm, is capable of deeply penetrating biological issue under irradiation of a long-wavelength monochromatic light source, possesses low toxicity and low side effect, and is high in singlet oxygen generation efficiency, and electron of the photosensitizer is easy for intersystem crossing.

The invention discloses an optical navigation system for surgeries, and relates to the field of medical equipment. The optical navigation system for the surgeries works in an visible light environment and comprises a fluorescence excitation light source, a visible light image collecting device, a near-infrared image collecting device, an image processing device and a display screen, the fluorescence excitation light source is capable of emitting invisible near-infrared light, the visible light image collecting device is capable of collecting visible light images formed after light emitted by a surgery illuminating device is reflected by a subject in a detect area, and the near-infrared image collecting device is capable of collecting near-infrared fluorescent images formed after light emitted by the fluorescence excitation light source is motivated by the subject in the detection area, wherein the wave length of the near-infrared fluorescent images is larger than 850 nm; the display screen is installed on a pair of goggles or a head-mounted support and used for displaying images processed by the image processing device. According to the device, fluorescence imaging is achieved in the visible light environment, the fluorescent images with high resolution and deep tissue are obtained, and imaging is clear, so that a doctor can learn about the real-time fluorescence imaging situations at any time without breaking surgical operation during the surgeries.

An object of the present invention is to provide a resin composition which can be detected both by X-ray radiation and by fluorescence or phosphorescence, and a molded article obtained from the resin composition. The present invention provides a resin composition containing a light-emitting substance and a radiopaque substance; in which the light-emitting substance is a near-infrared fluorescent material or a phosphorescent material. a radiopaque substance of the resin composition is any one of barium sulfate, bismuth oxide, bismuth subcarbonate, calcium carbonate, aluminum hydroxide, tungsten, zinc oxide, zirconium oxide, zirconium, titanium, platinum, bismuth subnitrate, and bismuth. A molded article can be obtained by processing any one of the resin compositions described above.

The invention discloses a near-infrared fluorescent molecular probe for detecting hydrogen sulfide and a preparation method and application of the near-infrared fluorescent molecular probe. The near-infrared fluorescent molecular probe has a molecular formula of C31H29N4O+, and has a structure formula shown in the description. Compared with the prior art, the near-infrared fluorescent molecular probe disclosed by the invention is good in selectivity and good in response effect on hydrogen sulfide, and in addition, can be well applied to living imaging and the like because of the properties ofnear-infrared absorption and fluorescence emission. In addition, the near-infrared fluorescent molecular probe is simple in synthesis process, low in cost and high in yield.

The invention discloses a near-infrared fluorescent dye based on coumarin structure and a synthesis method thereof. The general structural formula of the fluorescent dye is shown in the description: wherein R represents C1 alkyl group, X- represents PF6- or R represents any one of C2-C12 alkyl groups, X- represents Br-. 4-(diethylamino) coumarin is subjected to Vilsmeier-Haack reaction to synthesize 4-(diethylamino) coumarin aldehyde, 4-(diethylamino) coumarin aldehyde reacts with cyanopyridine and halogenated alkanes with different carbon chain lengths in turn to obtain a series of fluorescent dyes. The fluorescent dye of the invention can specifically label suborganelles in living cells through regulation and control of ammonium salt side chains, and the fluorescent dye with R representing C3 alkyl group can realize rapid dyeing and washing-free in the labeling process; the maximum emission wavelength of fluorescent dye is in the red luminescence region, which can significantly eliminate the interference of self-fluorescence phenomenon in cell imaging. In addition, fluorescent dyes with R representing C9 and C12 alkyl groups have the property of producing singlet oxygen and havecertain application potential in photodynamic therapy of cells and bacteria.

The invention contains a resin and a near infrared fluorescent material which is one type or two or more types of compounds selected from General Formulas (I1) to (I4) and has a maximum fluorescence wavelength of 650 nm or longer. In Formulas, Ra and Rb, Rc and Rd, Rh and Ri, and Rj and Rk form rings together with the nitrogen atom to which Ra, Rc, Rh, and Rj are bonded; Re and Rf represent a halogen atom or an oxygen atom; each of Rl, Rm, Rn, and Ro independently represents a halogen atom, a C1-20 alkyl group, a C1-20 alkoxy group, an aryl group, or a heteroaryl group; Rg, Rr, and Rs represent a hydrogen atom or an electron withdrawing group; and each of Rp and Rq independently represents a hydrogen atom, a halogen atom, a C1-20 alkyl group, a C1-20 alkoxy group, an aryl group, or a heteroaryl group.

The invention provides a near-infrared fluorescent heptamethine cyanine dye and application thereof in preparation of a tumor diagnosis agent and / or an imaging agent. The near-infrared fluorescent heptamethine cyanine dye is a compound DZ-1, wherein the side chain of the compound DZ-1 is (CH2)4SO3H, so that the compound DZ-1 can be directly dissolved in water; in addition, the near-infrared fluorescent heptamethine cyanine dye can be applied by intravenous injection during living imaging, thus achieving the same treatment and applying mode as clinic ICG (Indocyanine Green); the compound DZ-1 can be specifically absorbed by hepatoma carcinoma cell and is gathered at the tumor part to support no-intrusive NIRF imaging, so that the tumor growing part can be detected; the fluorescence intensity at the tumor part, the specificity and the targeting performance are prior to those of clinically-used ICG. Therefore, the tumor diagnosis agent and / or the imaging agent adopting the compound DZ-1 are high in tumor targeting performance and high in stability.

A nano-capsule construct for imaging and therapeutic uses and method for production are provided. One nano-probe embodiment based on genome-depleted plant brome mosaic virus (BMV) whose interior is doped with indocyanine green (ICG), an FDA-approved near infrared fluorescent dye, is used to illustrate the invention. The material encapsulated in viral shell components may be coated with functionalized coatings such as branched, dendritic polymer coatings to improve longevity and distribution in the body as well as antibody conjugation for increased target specificity. The constructs can also be coated with ferromagnetic iron oxide nanoparticles, enabling the ICG-containing capsules to be used as nano-probes with the capability of being detected in both optical and magnetic resonance imaging. The capsules may be produced by purifying a plant or animal viruses and disassembling the viruses to provide virus shell components. The virus shell components are reassembled in the presence of a material for encapsulation thereby encapsulating said material within the core of the construct in one embodiment.

The invention discloses a visible-light and near-infrared fluorescent 3D co-imaging endoscope system based on a single detector, and belongs to the technical field of endoscope imaging systems, achieving simultaneous acquisition and display of visible-light and near-infrared fluorescent 3D images. The 3D co-imaging endoscope system comprises a visible-light near-infrared excitation light source, abinocular endoscope imaging system, an optical relay inversion system, an image sensor module, an image processing fusion module and a 3D image display system. The endoscope system has the advantagesthat two images with horizontal parallax are received by the RGB-NIR detector to achieve acquisition of the 3D images, so that the visible-light colored 3D image and the near-infrared fluorescent 3Dimage are acquired at the same time; the system structure is simple, and the size is small; operation interruption for status switching is not needed, and smooth operation processes are ensured; the position and size of lesion tissue can be sensed by doctors directly, and accordingly, the success rate of operations is greatly increased.

The invention relates to the field of optical imaging, in particular to a radix stemonae alkaloid analogue near-infrared fluorescent probe and a preparation method thereof. The near-infrared fluorescent probe is applied to rapid detection of biological mercaptan, and is applied to imaging of the biological mercaptan in cells and living bodies. Existing reported fluorescent probes for detecting thebiological mercaptan have certain limitations, such as complex synthetic routes, long response time, short emission wavelength and the like. The preparation method of the near-infrared fluorescent probe comprises the following steps: 5-(hydroxymethyl)-7-methyl-3, 3 a-dihydrocyclopenta [ b ] chroman cyclo-1 (2H)-one and 3, 7-bis (dimethylamino)-10H-benzothiazine-10-carbonyl chloride react under the action of 4-dimethylamino pyridine and pyridine to generate (7-methyl-1-oxy-1, 2, 3, 3 a-tetrahydrocyclopenta [ b ] chroman cyclo-5-yl)-methyl-3, 7-bis (dimethylamino)-10H-benzothiazine-10-carboxylate.

This invention relates to a near infrared fluorescent probe for detecting hydroxyl free radicals, synthesis method and use thereof. The near-IR fluorescent probe, manufacture method and application for -OH Free Radical --a. adding the 2, 2, 6, 6-tetramethyl peridol and strong base by mole ratio as 1:0.5~2.5 into the DMF to dissolve and water-bath react for 25-40min with IG protection; b. adding Cyanine-7 into the solution by same mole rate with the DMF to obtain the Cyanine-7 solution; adding former mixed liquid into the current solution slowly within 10-20min for reaction 25-35min in dried brash ice; c. vacuum evaporating the product to remove the DMF, cleaning with aether, and vacuum drying to obtain the coarse product; d. separating with silica gel column chromatography for the deep-blue solid pure product.

The invention discloses near-infrared fluorescent dye THX-Np capable of achieving mitochondrial localization and a preparation method and application of the near-infrared fluorescent dye THX-Np. The structural formula of the dye is shown in (I). The dye has a stable pH fluorescence opening effect in an aqueous phase under the conditions of different pH values, and 2,4-dinitrophenyl ether derivatives prepared by using the dye can be applied to detection of hydrogen sulfide. The invention has the following advantages that the fluorescent dye has pH stability, large Stokes shift, good water solubility, good cell membrane permeability and excellent mitochondrial localization performance, and hydroxyl groups and carboxyl groups of the dye can be easily modified so as to produce tracers relatedto organisms, the 2,4-dinitrophenyl ether derivatives prepared through etherification of hydroxyl groups of the dye have excellent selective recognition of hydrogen sulfide, and an excellent selectivefluorescent probe of hydrogen sulfide detection.

The invention discloses a homogenous-phase fluorescent immune reagent for rapidly and quantitatively detecting trace albumin, and a preparation and detection method thereof. The reagent consists of anti-MAU marked by rare-earth element chelate and another anti-MAU marked by a near-infrared fluorescent compound, and a series of concentration MAU calibration products. By adopting the homogenous-phase fluorescent immune rapid detection technology, and utilizing the high-sensitivity characteristic of the fluorescence, the adverse influence of the non-homogeneity of pores of a nitrocellulose membrane in a colloidal gold or fluorescent MAU dry-type immune test paper on the detection accuracy and repetition can also be avoided, and the detection sensitivity and the linear range can be greatly improved; and the reagent provided by the invention is used for detecting trace albumin in human blood, serum or plasma, has the characteristics of simplicity and rapidness in operation, sensitivity, good specificity and the like, and has good clinical application prospect.