The invention relates to an
ELISA kit for detecting
zearalenone, the detection is rapid, sensitive, accurate, quantitative, simple in operation, low in requirements on sample purity and strong in specificity, thereby being particularly applicable to the detection of large quantities of samples; and the invention also provides a preparation of the kit and a detection method. The kit comprises washing liquid, color developing liquid A, color developing liquid B and stop solution, and the kit is characterized in that: the kit also comprises a coated plate, a
zearalenone standard product, a
zearalenone monoclonal antibody freeze-dried product and an
enzyme-labeled goat anti-mouse
antibody free-dried product; when in detection, the coated plate is taken, 50mu1-100mu1 of the ZEN
standard product or a well processed sample is added into the respective micropores, 50mul-100mul of the anti-ZEN
antibody is added, the incubation is carried out at 35 DEG C-45 DEG C for about 0.5 hour-1 hour, the washing liquid is used for washing for 3 times-5 times, 50mu1-100mu1 of the
horseradish peroxidase (HRP)-goat anti-mouse antibody is added, the incubation is carried out at about 35 DEG C-45 DEG C for about 0.5 hour-1 hour, the washing liquid is used for washing for 3 times-5 times, 50mu1-100mu1 of the color developing liquid A and 50mu1-100mu1 of the color developing liquid B are added, the mixture is placed still in the dark for 10 minutes-20 minutes, then the stop solution is added, the
absorbance value is measured at 450nm, and the ZEN content in the sample is calculated from a
standard curve.