Method for simultaneously detecting six sweetening agents in feed additive with high performance liquid chromatograph

Abstract

The invention relates to a method for simultaneously detecting six sweetening agents in a feed additive with a high performance liquid chromatograph. The six sweetening agents include A-K sugar, saccharin sodium salt, sodium cyclamate, aspartame, neotame and NHDC (Neohesperidin Dihydrochalcone). The method comprises the following steps: (1) preparing mixed standard working liquor; (2) preparing a sample solution to be detected; (3) using an instrument, namely the high performance liquid chromatograph; (4) adding ethyl alcohol in a used pretreatment method, such that a component NHDC difficultly dissolved in water in the sample is dissolved sufficiently; (5) drawing a standard curve; (6) analyzing a result. The method for simultaneously detecting the six sweetening agents in the feed additive with the high performance liquid chromatograph is simple in operation, accurate and reliable in detection result, good in repeatability, high in detection efficiency and excellent in separation effect, and can finish the detection of the six sweetening agents within 21 minutes while the pollution of a detection reagent to the environment in a detection process is reduced.

Description

technical field [0001] The invention relates to the field of additive detection, in particular to a detection method for sweeteners, in particular to a method for simultaneously detecting six kinds of sweeteners in feed additives with a high performance liquid chromatography. Background technique [0002] Sweeteners are widely used in the feed industry because they can mask the peculiar smell of feed ingredients and improve the palatability of feed. The sweetener currently used in the feed industry has changed from a traditional single sweetener (saccharin sodium) to a compound type, that is, saccharin sodium is mainly used, and synergists (A-K sugar, cyclamate, aspartame etc.) and long-acting sweeteners (new methyl hesperidin dihydrochalcone, neotame, etc.) The compound sweetener utilizes the taste time difference of different sweet ingredients in the oral cavity and the synergistic effect between the sweet ingredients to increase the sweetness of the sweetener, improve th...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many detection methods for sweeteners, the most common ones are high performance liquid chromatography-ultraviolet detection method, capillary gas chromatography, ultraviolet spectrophotometry, etc., but these detection methods are mostly for single sweetener or two sweeteners. Determination of odorant, detection sensitivity also needs to be improved

Therefore, when it is necessary to detect the sweetener components in the compound sweetener, it is necessary to use its own pretreatment technology and analysis method, and the detection is time-consuming, laborious, and expensive.

There are also reports on the detection of various sweeteners using ultra-high pressure liquid chromatography-time-of-flight mass spectrometer, ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer, but these two analytical instruments are expensive and not easy to be widely promoted in my country

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