The invention discloses a Chinese herbal medicine preparation for preventing and treating liver and gall diseases of aquatic animals as well as a preparation and use method thereof, and relates to the field of aquatic animal breeding. The compound Chinese herbal medicine preparation for preventing and treating the liver and gall diseases of the aquatic animals comprises the following components in parts by weight: 8-15 parts of schisandra chinensis, 6-12 parts of cynara scolymus, 6-12 parts of silybum marianum, 10-15 parts of radix bupleuri and 9-12 parts of radix paeoniae alba. The preparation method comprises the following steps: weighing raw materials, respectively crushing and sieving the raw materials to obtain dry powder, and mixing the dry powder to obtain the Chinese herbal medicine preparation. According to the use method, the Chinese herbal medicine preparation is mixed into aquatic feed for use, and the additive amount of the Chinese herbal medicine preparation in the aquatic feed is 2-3% of the weight of the aquatic feed. The formula is reasonable, the theory of traditional Chinese medicine is scientifically applied, different Chinese herbal medicines have a good prevention and treatment effect on the liver and gall diseases of the aquatic animals through mutual synergistic effect, toxic and side effects are small, water area environment pollution is not likely to be caused, the Chinese herbal medicine preparation is safe, pollution-free, residue-free, green, healthy and environment-friendly, and the aquaculture benefits can be effectively improved.