The invention relates to the technical field of medicines, and discloses a method for preparing high-purity
rutin via a two-dimensional liquid
chromatography-
mass spectrometry combined technology. The method comprises the following steps of: adopting the two-dimensional liquid
chromatography-
mass spectrometry combined technology, taking
methanol-water or
acetonitrile-water as a mobile phase, using a reversed
chromatographic column as the first dimension for preparing a
chromatographic column, performing component
cutting on a
ginkgo leaf extract, and collecting a target component, namely, a
rutin crude product; and using the reversed
chromatographic column as the second dimension for preparing a chromatographic column, performing component
cutting on the
rutin crude product under the guide of a
mass spectrometry selected
ion peak, collecting, rotationally evaporating and concentrating, and then obtaining the high-purity rutin having a purity which can achieve more than 80%. In the method disclosed by the invention, heating is not needed, so that the
doping of a
hydrolysate is avoided; the preparation is simple in process, high in
repeatability, and good in
operability; simultaneously,
ginkgo leaves are abundant in resource and easy to obtain, thus reducing the cost; and the method is suitable for large-scale production.