The invention relates to a
wheat germ protein source
antioxidative peptide, and a preparation method and an application thereof. The
wheat germ protein source
antioxidative peptide comprises sequences shown as SEQ ID NO.1 and SEQ ID NO.2. The preparation method comprises the steps of making fresh wheat germs into defatted
wheat germ protein powder; hydrolysizing with an
alkaline protease, inactivating
enzyme, centrifuging at a high speed, subjecting supernatant to
ultrafiltration so as to remove large molecular substances, freeze-
drying filtrate to obtain a wheat germ protein
hydrolysate, passing a wheat germ protein
hydrolysate solution through a macroporous absorbent resin column and a gel
filtration chromatography column successively, and collecting components with the strongest
antioxidant activity being a target product. The preparation method is simple and efficient in process. The obtained wheat germ protein source
antioxidative peptide has relatively high
antioxidant activity, has relatively small molecular weight and definite
amino acid sequence, can clear free radicals and alleviate
oxidative stress injuries, can also be used for
antioxidant dietary supplements, and has the advantages of wide
raw material sources,
high security, low cost and the like, and has wide application prospects in the fields of foods, medicines, etc.