105 results about "Cordyceps sobolifera" patented technology

The invention discloses a method for artificially culturing paecilomyces cicadae and application of a culturing product thereof. The method for artificially culturing paecilomyces cicadae in large scales comprises the steps of: preparing strains, dosing and packing into a box, sterilizing, inoculating, solid-fermenting, collecting and the like. The paecilomyces cicadae is cultured by utilizing grains, such as corn flour, bran, wheat, barley, rice, millet, broomcorn and the like and culture mediums of bagasse, cane sugar, shell powder, silkworm chrysalis meal and potassium nitrate. The raw materials are obtained from local resources, a large amount of production cost is saved, the culturing period is shortened, and cordyceps sobolifera obtained by culturing has the advantages of high quality and favorable stability and the like. A culture obtained by the invention can be used for preparing foods, health-care products, drugs and cosmetics with functions of fighting tumor, regulating immunity, reducing blood sugar, blood fat and blood pressure, improving eye sight, resisting radiation, dispelling heat and easing pains, calming and hypnotizing, nourishing and strengthening, improving kidney function and the like.

The invention discloses a bionic culture method for cordyceps sobolifera, which includes fungus separation, screening, spore culture, infected body impregnation, medium culture, harvest and other steps and culture conditions thereof. The method can simulate the wild ecological environment and overcome the limit of the growth period and season of wild cordyceps sobolifera, mass culture is easy, the adenosine content of the cultured bionic cordyceps sobolifera is higher than the adenosine content of wild cordyceps sobolifera, the quality is high, and the bionic cordyceps sobolifera has extremely high market popularization value.

The invention discloses a preparation method of a cordyceps sobolifera and burdock oral liquid, and belongs to the technical field of extraction of nutritional components of plants. The preparation method of the cordyceps sobolifera and burdock oral liquid comprises the following steps: (1) preparing cordyceps sobolifera cordyceps liquid seeds; (2) cultivatiing cordyceps sobolifera cordyceps; (3) extracting after the materials are smashed with hot water and carrying out homogenate; (4) carrying out high shear and superfine grinding with wet method; (5) hydrolyzing cellulose, amylase, and pectinase; (6) filtering, concentrating and filling to obtain the cordyceps sobolifera and burdock oral liquid. The preparation method is short in product production cycle, avoids raw material loss in product production process and waste discharge in production process, and is an environment-friendly production method with a high product output rate and simple production technology; besides, as health care type functional food, the cordyceps sobolifera and burdock oral liquid can be drunk for a long time as daily health care, can be used as a product for the adjuvant therapies of related diseases, and broadens the application fields of cordyceps sobolifera cordyceps and burdock root.

The invention discloses a coffee flavor health-maintaining sea cucumber and dendrobium officinale solid beverage. The coffee flavor health-maintaining sea cucumber and dendrobium officinale solid beverage is prepared from the following raw materials in parts by weight: 4-6 parts of sea cucumbers, 8-10 parts of ground coffee, 22-25 parts of dendrobium officinale, 11-13 parts of broccolis, 3-5 parts of yellow rice or millet wine, 9-11 parts of rock candy, 1-2 parts of erycibe obtusifolia benth, 7-9 parts of amomum kravanh, 10-12 parts of tomato juice, 8-10 parts of raspberries, 3-4 parts of cordyceps sobolifera, 14-16 parts of buckwheat powder, 1-2 parts of fennels, 6-7 parts of pollen pini, 6-7 parts of walnut protein powder, 7-9 parts of corn soybean milk, 3-4 parts of enteromorpha, 1-2 parts of lysimachia christinae hance, 9-12 parts of a nutritional supplement and a suitable amount of water. The coffee flavor health-maintaining sea cucumber and dendrobium officinale solid beverage has rich coffee flavor, and savory and mellow taste; dendrobium officinale has the effects of promoting the secretion of saliva or body fluid and nourishing stomach, moistening lung and tonifying kidney, and improving eyesight; furthermore, the content of proteins, calcium, potassium, zinc, iron, selenium and manganese is high and a plurality of amino acids are contained, so that the beverage has the effects of enriching blood and moistening dryness; and the coffee flavor health-maintaining sea cucumber and dendrobium officinale solid beverage is suitable for people with low immunity, anepithymia, palpitation and shortness of breath and dizziness to drink and has high nutritive value and good health effect.

The invention discloses a liquid fermentation culture medium for a high-yield antioxidant cordyceps sobolifera mycelium and a production method of antioxidant cordyceps sobolifera mycelium granules. The culture medium comprises the following components in percentages by mass: 1-5% of glucose, 1-10% of a high molecular carbon source, 1-10% of a nitrogen source and 0-0.5% of an inorganic salt respectively, distilled water is used as a solvent, and the pH is natural. The high-yield cordyceps sobolifera mycelium is obtained by improved fermentation through the liquid fermentation culture medium for the high-yield antioxidant cordyceps sobolifera mycelium, the biomass content is high, the contents of effective components including polysaccharides and mannitol are high, the antioxidant activityis strong, and the source of health food raw materials is increased; meanwhile, the cordyceps sobolifera mycelium is obtained by a liquid fermentation technology, the production cost is low, the period is short, the limitation of environmental conditions is avoided, and the cordyceps sobolifera mycelium is suitable for large-scale industrial production and popularization; and the high-yield antioxidant cordyceps sobolifera mycelium granules produced by the invention have effects of easiness in absorption and utilization, convenience in carrying and strong nourishing.

The invention discloses an artificial cultivation method of cordyceps sobolifera. The method comprises the following steps: soaking mature nymphs of cicadas obtained by either field acquisition or artificial cultivation with pure water for 5 min, washing, and drying on a filter screen; uniformly spraying paecilomyces cicadae strain diluted solution, of which the total active bacteria number is 1*108, obtained by the culture and expanding propagation in a culture chamber, to the dried nymphs by a sprayer according to 5% of the weight of the nymphs; moving the re-dried nymphs into a cordyceps sobolifera cultivation plate, covering a plate cover, and cultivating in a sterile room at the temperature of 24-26 DEG C and the relative air humidity of 80-90%; after the heads of the nymphs grow cordyceps sobolifera which is similar to flowers, and taking out and drying the cordyceps sobolifera to obtain the artificially cultivated cordyceps sobolifera. Meanwhile, the invention discloses a cultivation plate for artificially cultivating the cordyceps sobolifera; in addition, by adopting the artificial cultivation method of cordyceps sobolifera to cultivate the cordyceps sobolifera, the industrial production of the cordyceps sobolifera can be realized, and the increasingly grown dietary therapy and clinical demands can be met.

The invention discloses cordyceps cicadae / cordyceps sobolifera mycelium active substance and a composition for protecting nerve cells. Under the noncytotoxic concentration of 40 mu / ml, the cordyceps cicadae / cordyceps sobolifera mycelium active substance can be used for protecting the neurotoxicity of MPP+ to the dopaminergic neuron, and also can be used for inhibiting the cell apoptosis caused by the pressure of the endoplasmic reticulum caused by tunicamycin, and therefore, the cordyceps cicadae / cordyceps sobolifera mycelium active substance has the potential of preventing neurodegenerative diseases.

The invention discloses a culture method of selenium-enriched cordyceps sobolifera conidial powder. Firstly, separation, purification and identification are performed on cordyceps sobolifera collected in a field to obtain Paecilomyces cicadae (Miquel.) Samson, plate cultivation is performed to obtain single colonies, then, the single colonies are transferred to a liquid medium for culture, a liquid secondary seed solution is obtained, the secondary seed solution is transferred to a composite culture medium containing organic selenium fertilizer, the temperature is controlled to 15-35 DEG C, the humidity is controlled to 60-90%, ventilation culture is performed for 4-6 weeks, and harvesting is performed to obtain selenium-enriched cordyceps sobolifera conidial powder. Artificial simulation is performed to obtain nutritional and growth conditions closest to the conditions needed by cordyceps sobolifera wild environment, growth of cordyceps sobolifera is not limited by season changes, the composite culture medium is closer to natural bamboo forest cordyceps sobolifera wild environment, the breathability is good, the water absorption and moisturizing performance are excellent, a fruiting body grows rapidly, spore head and conidial powder of the fruiting body are abundant, thick, solid, full, large in conidial powder amount, high in quality and easy to harvest, the content of selenium is 20-100 mg / Kg, the method is simple and low in cost, the spore harvesting period is short, and industrial production can be achieved.

The invention relates to a Cordyceps sobolifera active site and application thereof in preparing drugs for nerve protection and aging resistance. The active site is an n-butanol site of a Cordyceps sobolifera water extract. The active site is prepared by the following steps: pulverizing Cordyceps sobolifera, extracting with water under reflux, concentrating the extracting solution under reduced pressure, adding anhydrous ethanol, and precipitating over night; centrifuging, and concentrating; and carrying out fractional extraction on the concentrated solution with petroleum ether, ethyl acetate and n-butanol, concentrating under reduced pressure to recover the solvent, and carrying out freeze-drying to respectively obtain a petroleum ether site, an ethyl acetate site, an n-butanol site and a water site. The n-butanol site can obviously inhibit aging and damage of the glutamic-acid-induced PC12 cells, prevent the cell LDH from release, enhance the survival rate of the cells, lower the intracellular oxygen free radical level, enhance the activity of the glutathione reductase (GSH-Px) and superoxide dismutase (SOD), and have favorable in-vitro oxidation resistance activity in the experiment of clearing DPPH. and superoxide anion (O<2.->) free radicals.

The invention relates to an artificial cultivation method of cordyceps sobolifera. The method comprises the following steps: step 1, expanded cultivation of cordyceps sobolifera strains; step 2, soil culture of strains obtained by expanded cultivation in the step 2, step 3, harvesting of coremium. In the step, the soil culture comprises dark culture and light culture phases. In the light culture, green light is utilized for irradiation. The method has the following beneficial effects: in the phase of cordyceps sobolifera cultivation, green light (single-color light) is utilized for irradiation; compared with white composite light, the yield of coremium is greatly increased.

The invention discloses a method for preparing health cordyceps sobolifera yellow wine with a cordyceps sobolifera culture medium, and belongs to the technical field of biology. The health cordyceps sobolifera yellow wine is prepared by adding the cordyceps sobolifera culture medium which is subjected to high-speed crushing and ultrasonic cell breakage and cylinder flushing water at the same time during cylinder flushing for fermentation with sticky rice serving as a raw material, to fully extract effective components of cordyceps sobolifera in the cordyceps sobolifera culture medium. Therefore, the prepared health cordyceps sobolifera yellow wine has certain effects of enhancing the immunity of a human body, resisting aging and preventing diseases; meanwhile, due to the full use of the cordyceps sobolifera culture medium prepared from harvested and cultured cordyceps sobolifera, the economic benefit of cordyceps sobolifera cultivation is increased.

The invention belongs to the technical field of artificial cultivation of edible mushrooms, and particularly relates to an artificial cultivation method for increasing the content of polysaccharide in cordyceps sobolifera sporocarp. According to the cultivation method for increasing the content of the cordyceps sobolifera polysaccharide in the cordyceps sobolifera sporocarp, on the basis of a conventional cordyceps sobolifera sporocarp cultivation process, dead leaves and pruned spindling leaves generated in succulent plants bred by themselves are added into a solid culture medium of cordyceps sobolifera, it is surprisingly found that in the solid fermentation process of cordyceps sobolifera, the dead leaves and leaves of the added succulent plants can effectively induce accumulation of the cordyceps sobolifera polysaccharide, and the content of the cordyceps sobolifera polysaccharide in the cordyceps sobolifera sporocarp is increased.

The invention discloses cordyceps sobolifera dried meat floss and a making method thereof, the dried meat floss is made from cordyceps sobolifera powder and soy isolate protein as raw materials, the making method is as follows: mixing the cordyceps sobolifera powder and the soy isolate protein; while stirring adding water; systematizing by high moisture extrusion; adding into a pot with boiling water for boiling, adding an edible condiment; frying and pressing; drying and dehydrating after frying and pressing, putting into a dried meat floss frying machine for processing; cooling, sterilizing, packaging, and discharging. Compared with the prior art, the making method has the following advantages: the prepared dried meat floss is low in energy, free of cholesterol, animal fat, and external fat, rich in unsaturated fatty acids and vitamin E, and rich in dietary fiber and nutrition, and can provide adequate dietary fiber and nutrients daily required by the human body.

The invention provides a fermentation method of antioxidant cordyceps sobolifera mycelia. The fermentation method comprises the following steps: respectively preparing a PDA slant culture medium, a seed culture solution and a fermentation culture medium; dividing the paecilomyces cicadae mother strain through sterile operation, and inoculating the paecilomyces cicadae mother strain to the PDA slant culture medium for activation culture to obtain an activated strain; inoculating the activated strain into a seed culture solution to obtain a primary strain; inoculating the primary strain into theseed culture solution, and culturing the primary strain according to a method for culturing the activated strain to obtain a secondary strain; inoculating the primary strain or the secondary strain into the seed culture solution for culturing to obtain a fermentation tank seed solution; inoculating the fermentation tank seed solution into the fermentation culture medium, and carrying out fermentation culture to obtain cordyceps sobolifera fermentation liquor; and discharging the cordyceps sobolifera fermentation liquor, and carrying out semi-solid fermentation culture to obtain the fermentation product of the antioxidant cordyceps sobolifera mycelia. The fermentation method is suitable for fermentation production of cordyceps sobolifera mycelia, the obtained mycelia is high in biomass andstrong in antioxidant activity, the yield of active substance polysaccharide and cordycepic acid can be remarkably increased, and the source of health-care food raw materials is increased.

The invention relates to a new use of Cordyceps sobolifera, especially a use of Cordyceps sobolifera or its fruiting body in preparing medicament proliferating osteocyte. The invention opens a new direction in the research for application of Cordyceps sobolifera, and brings gospel for patients with osteoporosis.

The invention belongs to the field of traditional Chinese medicine production and relates to an application of Cordyceps sobolifera water extract in preparation of an acute liver injury preventing and treating drug. Based on D-galactosamine / lipopolysaccharide induced acute liver injury model experiments, results prove that the Cordyceps sobolifera water extract can relieve lipid peroxidation and oxidative stress injury of liver cells by reducing transaminase (AST and ALT) and alkaline phosphatase (ALP) in serum, remarkably relieves infiltration of inflammatory factors of the liver and improves liver lesion obviously, meanwhile, the Cordyceps sobolifera water extract can relieve infiltration of neutrophils in the liver, inhibit transcription and expression of related inflammatory mediators, reduce inflammation induced inflammatory factor and NO release and inhibit MAPK (mitogen-activated protein kinases) pathway activation. The Cordyceps sobolifera water extract can be used for preparing the acute liver injury preventing and treating drug and is used for protecting liver function and relieving liver inflammation.

The invention discloses paecilomyces cicadae and application in preparing an antibacterial agent, a probiotic preparation and an antibacterial fresh-keeping film. A novel strain-paecilomyces cicadae CH 113.1 is first separated from field entomogenous fungus cordyceps sobolifera. A strain fermentation product has an inhibition effect for conventional pathogenic microorganisms, the inhibition rate for the escherichia coli can reach 70 percent or more, and the biological bacteriostatic agent can be applied to the fresh keeping of food. The strain has a good application prospect in the field of food.

The invention discloses a method for using a bamboo forest to cultivate cordyceps sobolifera. The method includes: building a site suitable for the gathering and living of cicadas, sowing cordyceps sobolifera strains in the bamboo forest after the cordyceps sobolifera strains are cultivated artificially, using light to attract the cicadas together to allow cicada larva to be infected by the cordyceps sobolifera strains when the cicada larva falls into the ground so as to obtain cordyceps sobolifera, in other words, selecting the bamboo forest suitable for the cicadas to live so as to create good environmental conditions for copulation, egg laying and larva growth of the cicadas, and allowing the cordyceps sobolifera strains to reproduce massively in the bamboo forest through artificial cultivation and cordyceps sobolifera strain sowing so as to allow the cicada larva to be infected by the cordyceps sobolifera strains when the cicada larva falls into the ground and increase the yield of the cordyceps sobolifera. The method has the advantages that the natural bamboo forest is fully utilized to create the environment which is benefit for the living of the cicadas, the number of the cordyceps sobolifera strains is increased through artificial sowing, yield of the natural cordyceps sobolifera is increased, the cordyceps sobolifera can be harvested for several years after the one-time sowing, and promising application prospect is achieved.

The invention relates to the technical field of eye protecting masks, particularly to an eye protecting composition, an eye mask thereof and a preparation method of the eye protecting composition. Theeye protecting composition takes cordyceps sobolifera extract, puerarin, beta-carotene, silymarin, cannabis oil, beta-glucan, tremella fuciformis extract and the like as principal raw materials whichsynergize with one another, so that the eye protecting composition can contain small-molecular active components required by eyes such as cordycps sobolifera polysaccharides, beta-glucan, acidic polysaccharoses, neutral heteropolysaccharides and acid oligosaccharides to provide rich energy sources for circumocular cell repair; effective constituents contained in the specifically-added cannabis oil such as cannabidiol, taurine and puerarin can achieve unique effects of calming optic nerves and promoting microcirculation; when combined with a microcurrent device, the eye mask can achieve the special effects of microcurrent induction to promote absorption and utilization of effectively constituents by circumocular acupuncture points and further to enhance the effects of controlling and correcting vision and repairing eye cells.

The invention relates to the field of biotechnology and discloses a production method for Cordyceps sobolifera mycelium and exopolysaccharide liquid fermentation. The production method comprises: using a strain of Cordyceps sobolifera as a starting strain, and acquiring mycelium and exopolysaccharide by liquid deep fermentation; grinding a test tube slant strain under aseptic condition, inoculating to a liquid medium to prepare a liquid strain, subjecting the liquid strain to liquid deep fermentation, shake culturing or stir culturing to obtain a culture, and separating mycelium and a culture solution by means of centrifugation or filtration and the like to obtain mycelium (dry weight) 5-8 g / L; subjecting the culture solution to ultrafiltration or ethanol precipitation to obtain exopolysaccharide, 0.5-1.2 g / L in yield. The production of Cordyceps sobolifera mycelium and exopolysaccharide may be carried out using the above method, and this method has high development value.

The invention belongs to the technical field of traditional Chinese medicine, and particularly provides traditional Chinese medicinal eye drops for treating eye diseases. The traditional Chinese medicinal eye drops for treating the eye diseases are prepared from the following raw materials in parts by weight: 15 to 20 parts of pearl powder, 5 to 12 parts of fructus viticis, 10 to 15 parts of apricot leaves, 8 to 12 parts of polygonum multiflorum, 11 to 15 parts of medlar, 13 to 24 parts of goldthread, 5 to 10 parts of parmelia saxatilis, 10 to 12 parts of safflower carthamus, 3 to 10 parts of cape jasmine, 12 to 18 parts of cassia occidentalis, 5 to 15 parts of cordyceps sobolifera, 15 to 20 parts of sedum lineare, 12 to 15 parts of elettaria cardamomum, 18 to 20 parts of digitaria sanguinalis, 8 to 15 parts of lance asiabell root, 5 to 12 parts of lily, 8 to 15 parts of honeysuckle, 6 to 14 parts of cortex dictamni, 12 to 18 parts of the root of red-rooted salvia, and 10 to 15 parts of roughhaired holly root. The traditional Chinese medicinal eye drops for treating the eye diseases provided by the invention are good in medicinal cure effect, quick in effect and low in cost, act on eyes directly, do not have a toxic or side effect, and particularly have very remarkable treatment effects on the eye diseases, such as conjunctivitis, hordeolum and trachoma; therefore, the traditional Chinese medicinal eye drops are worth being popularized and applied widely.

The invention relates to a method for cultivating calcium-rich cordyceps sobolifera. The method comprises the followings steps that 1, a cordyceps sobolifera strain is subjected to expansion cultivation in a liquid medium; 2, a solid medium is inoculated with the strain amplified in step 1 for solid culture; 3, coremium is harvested. The method is characterized in that calcium salt is added into the liquid medium in step 1 and / or the solid medium in step 2, and the calcium salt is any one or more of calcium citrate, citric acid-calcium malate, calcium acetate, calcium gluconate and calcium lactate.

The invention relates to cordyceps sobolifera micropowder tablets and a preparation method thereof. The cordyceps sobolifera micropowder tablets are tablets prepared from cordyceps sobolifera micropowder with the water content of 5% to 15%, the mesh of 1,000 to 10,000 and the particle size of 1.5 micrometers to 15 micrometers. The preparation method of the cordyceps sobolifera micropowder tablets comprises the following steps that 1, cordyceps sobolifera is crushed until the particle size is 1.5 micrometers to 15 micrometers and the water content is controlled to be 5% to 15%; 2, cordyceps sobolifera obtained in the step 1 is subjected to dry granulating, sieved with a 30-60-mesh sieve and then tableted, wherein a tabletting method comprises the step that the cordyceps sobolifera powder is prepressed before being tableted, the prepressing pressure is 0.8 kN to 30 kN, and the main pressure is 30 kN to 80 kN. According to the cordyceps sobolifera micropowder tablets and the preparation method thereof, the particle size of the powder obtained before cordyceps sobolifera tabletting is 1.5 micrometers to 15 micrometers, the dissolution rate is very high, the dissolution time is short, and the bioavailability is high.

The invention relates to the field of traditional Chinese medicine application and discloses a traditional Chinese medicine for treating liver and gallbladder damp-heat-type gallstone. The traditional Chinese medicine for treating liver and gallbladder damp-heat-type gallstone is prepared from Brandisia herb, medulla stachyuri, virgate wormwood, herb of common achyranthes, avicennia pricklyash root, broom cypress fruit, stringy stonecrop herb, Juncus roemerianus, semen nigellae, polyporus, hovenia dulcis thumb, coix seeds, rhizoma dioscoreae septemlobae, caulis clematidis armandii, fructus polygoni orientalis, curcuma tuber, snake bramble seed, rabdosia rubescens, imperata rhizome, pagodatree pod, biota orientalis, herba diplazii lancei, madder, herba cephalanoploris, bryophyllum, garden burnet, spiny amarauth herb or root, Chinese iris seed, pearl shell, cordyceps sobolifera, dogbane leaf and green tangerine peel as raw materials by mixing according to a certain ratio. The traditional Chinese medicine has the advantages of simple preparation processes, good absorption effects, long drug action time, substantial curative effects, no side effect, high safety, low production cost and timely remission of patient diseases.

The invention relates to bat dung heat clearing and eyesight improving tea and a preparing method thereof. The bat dung heat clearing and eyesight improving tea is prepared from tea and Chinese herb extracts by the mass ratio of 1:4, and the Chinese herb extracts are prepared from, by weight, 20-40% of bat dung, 10-20% of pleioblastus amarus leaves, 10-15% of cordyceps sobolifera, 10-15% of semen cassia, 5-10% of nacre mother of pearl, 5-10% of butterflybush flower, 5-10% of buerger pipewort flower, 3-5% of coral, 3-5% of cypraeae concha and 3-5% of edgeworthia chrysantha lindl. The preparing method includes the main steps of a preparing materials, b extracting filter liquor, c carrying out centrifugal separation and concentration, d smearing and drying and e parching and sterilizing. The bat dung heat clearing and eyesight improving tea has the effects of clearing heat, improving eyesight, dispersing extravasated blood, removing qi stagnation and eliminating corneal opacity after being drunk for a long time.

The invention discloses a solid medium for producing plasmin by using cordyceps sobolifera and a plasmin production method using the same, belonging to the field of microbial technology. The solid medium comprises the following active ingredients in parts by weight: 8-15 parts of peptone, 30 parts of glucose, 1 part of monopotassium phosphate, 3 parts of yeast extract, 0.2 part of magnesium sulfate heptahydrate, 15g of agar and 1000 parts of water. A plasmin production method using the solid medium for producing plasmin by using cordyceps sobolifera comprises the following steps: (1) inoculating a paecilomyces cicadidae strain (B5) to a PDA panel and culturing; (2) sterilizing the prepared solid medium and pouring into a sterilized petri dish; cooling to obtain a solid panel; teasing the hypha block with a teasing needle, inoculating to the prepared solid inducing medium panel and culturing; and (3) putting the cultured bacterial colony in a refrigerator overnight; fetching the hypha block with medium and adding into sterile water; mashing and standing; performing high-speed centrifugal separation; and fetching the supernate as a crude extract of plasmin for later use.

The invention discloses a traditional Chinese medicine liquid for treating trachoma and a preparation method thereof, and belongs to the field of a traditional Chinese medicine. The effective components of the traditional Chinese medicine liquid are prepared from the following raw materials: lichen of parmelia saxitilis, nomame senna herb, sunglow, lotus root, medlar, burdock, cordyceps sobolifera, borneol, radix ilicis asprellae, dichrocephala auriculata, apium graveolens, orchis, crab grass, fructus viticis, jasmine and henon bamboo sheath. The traditional Chinese medicine liquid is appropriate in compatibility of selected medicinal materials, accords with the theories of the traditional Chinese medicine and pharmacy and the modern medicine and pharmacology, has the effects of clearing away heat and toxic materials, enriching blood and brightening eyes, and dispelling wind and heat, has significant curative effects on the symptoms such as trachoma, dry eyes, pain and the like, is low in price, convenient to use, safe and reliable, free of toxic and side effects, effective and practical, and does not affect study, work and life after being used, not only can effectively relive dry eyes and mild discomfort, but also can play the roles of moistening and improving eyesight, and is worthy of extensive popularization and application.

The invention discloses a Cordyceps sobolifera protease capable of inhibiting human immunodeficiency virus-1 (HIV-1) reverse transcriptase and a preparation method for the Cordyceps sobolifera protease. The preparation method comprises the following steps of: 1) breaking Cordyceps sobolifera cells; 2) collecting a crude protein extract; and 3) separating and purifying protease from the crude protein extract collected in the step 2) to obtain a Cordyceps sobolifera extract. The protease has the molecular weight of 31KD, has the N-terminal amino acid sequence shown as a sequence 1 in a sequencetable, belongs to serine protease, and has the highest activity under the following conditions: the pH value is 12-12.5 and the temperature is 55-65DEG C. The Cordyceps sobolifera extract prepared bythe method has obvious inhibition effect on the HIV-1 reverse transcriptase, and has important value in the field of new anti-AIDS (acquired immune deficiency syndrome) medicine research.

The invention discloses Cordyceps sobolifera mycelium for preventing, delaying or treating cataract, an active substance thereof, a mycelium liquid fermentation product thereof, a pharmaceutical composition thereof, and a method for preparing the product. The Cordyceps sobolifera mycelium, the active substance and the mycelium liquid fermentation product can be prepared as a health food for preventing or delaying cataract.

The invention discloses an anti-helicobacter pylori probiotic composition as well as a preparation method and application thereof. The probiotic composition is prepared from the following raw materials in parts by weight of 60 to 80 parts of oat beta-glucan, 10 to 20 parts of probiotics, 1 to 4 parts of cordyceps sobolifera sporocarp, 1 to 4 parts of rice bran fatty alkanol, 1 to 4 parts of eucommia ulmoides male flower and 1 to 4 parts of psyllium husk. According to the composition, the oat beta-glucan is adopted to achieve the effect of protecting the probiotics, the dosage of the probiotics can be reduced, and the oat beta-glucan can promote the growth of the probiotics, so that the probiotics play a synergistic role; and meanwhile, the contained cordyceps sobolifera sporocarp (artificially cultivated), rice bran fatty alkanol, eucommia ulmoides male flower and psyllium husk all have the effect of inhibiting helicobacter pylori, so that helicobacter pylori infection is effectively prevented and improved.