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Production method for Cordyceps sobolifera mycelium and exopolysaccharide liquid fermentation

A technology of exopolysaccharides and mycelia, which is applied in the field of liquid fermentation production of the mycelium and exopolysaccharides of Cicada chinensis, and can solve the problems of unreported research on liquid culture of Cicada chinensis

Active Publication Date: 2016-05-11
NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, according to the difference in biology between the two, especially the growth rate, it can be determined that the current relevant "Cicadae" (partly used C. sobolifera The scientific name) reported that most of the reports are actually big cicadae ( C. cicadae ), but there is no report on the liquid culture of Cicada

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] 1. Cultivation of mother species

[0017] Inoculate the slant bacteria on the PDA medium, 25 o C culture in the dark for 25 days, the diameter of the colony grows to 4-5cm.

[0018] 2. Preparation of liquid strains

[0019] Pick out the well-grown slant bacteria, that is, the mother species, with an inoculation needle, transfer them to a sterile mortar, add sterile water at a rate of 5 mL for each strain, and grind to prepare a strain suspension, then Connect the suspension to the primary liquid medium GPBY (glucose 20g, peptone 5g, beef extract 4g, yeast extract 1g, water 1000mL, pH6.0-6.5) at a volume ratio of 1%, and the container capacity is 40 %, 25 o Under C, vibrating culture, the shaking table speed is 120r / min, cultivate for 10 days, until the mycelia ball is filled with the liquid medium, it can be used as a liquid strain.

[0020] 3. Liquid culture

[0021] Liquid culture was carried out by shaking culture. The above liquid strains were inoculated into ...

example 2

[0025] 1. Cultivation of mother species

[0026] Inoculate the slant bacteria on the PDA medium, 20 o C culture in the dark for 30 days, the diameter of the colony grows to 4cm.

[0027] 2. Preparation of liquid strains

[0028] Pick out the well-grown slant bacteria, i.e. the mother species, with an inoculation needle, transfer them to a sterile mortar, add sterile water at a rate of 6 mL for each bacteria, and grind to prepare a suspension of bacteria, and then Connect the suspension to the liquid seed medium GPBY (glucose 15g, peptone 4g, beef extract 4g, yeast extract 0.5g, water 1000mL, pH 6.0) at a volume ratio of 5%, and the volume of the container is 30%. 20 o Under C, vibrating culture, the shaking table speed is 130r / min, cultivate for 8 days, until the mycelia ball is filled with the liquid medium, it can be used as a liquid strain.

[0029] 3. Liquid culture

[0030] Liquid culture was carried out by shaking culture. Inoculate the above liquid bacteria into t...

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Abstract

The invention relates to the field of biotechnology and discloses a production method for Cordyceps sobolifera mycelium and exopolysaccharide liquid fermentation. The production method comprises: using a strain of Cordyceps sobolifera as a starting strain, and acquiring mycelium and exopolysaccharide by liquid deep fermentation; grinding a test tube slant strain under aseptic condition, inoculating to a liquid medium to prepare a liquid strain, subjecting the liquid strain to liquid deep fermentation, shake culturing or stir culturing to obtain a culture, and separating mycelium and a culture solution by means of centrifugation or filtration and the like to obtain mycelium (dry weight) 5-8 g / L; subjecting the culture solution to ultrafiltration or ethanol precipitation to obtain exopolysaccharide, 0.5-1.2 g / L in yield. The production of Cordyceps sobolifera mycelium and exopolysaccharide may be carried out using the above method, and this method has high development value.

Description

technical field [0001] The invention relates to the technical field of liquid fermentation and cultivation of large fungi, Cordyceps sobolifera ) liquid fermentation production method of mycelium and exopolysaccharide. Background technique [0002] Both traditional medicine and modern scientific research have shown that Cordyceps has extremely high health care and medicinal value. It has antibacterial, anti-tumor, anti-oxidation, nourishing yin and strengthening yang, protecting liver and lungs, and improving immunity. Thereby more and more be subjected to liking of people, market demand is constantly expanding. Cordyceps is a kind of fungi complex formed by parasitic fungi on insects and their host insects. Taxonomically, it belongs to Ascomycota, Pyenomycetes, Clavicipitaceae, Cordyceps ( Cordyceps). So far, there are more than 500 kinds of Cordyceps known in the world, and 130 kinds are known in my country, such as: Cordyceps militaris ( C. militaris ), Taiwan Cord...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/04C12R1/645
CPCC12N1/14C12P19/04
Inventor 林群英孙晓明张卫明吴亮亮张锋伦吴素玲
Owner NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP
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