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Cordyceps cicadae/cordyceps sobolifera mycelium active substance and composition for protecting nerve cells

A technology of active substances and mycelium, which is applied in the field of active substances of mycelium of cicadae and compositions for protecting nerve cells, can solve problems such as no obvious characteristics, and achieves improvement of neurodegeneration symptoms, improvement of neurotoxicity, treatment or prevention Effects of high neurodegeneration and its complications

Active Publication Date: 2016-06-29
GRAPE KING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since Parkinson's disease is caused by a lack of dopamine, there are no obvious characteristics at the initial stage of the disease, and it is often five years before the case is confirmed, and there is currently no effective drug to treat it

Method used

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  • Cordyceps cicadae/cordyceps sobolifera mycelium active substance and composition for protecting nerve cells
  • Cordyceps cicadae/cordyceps sobolifera mycelium active substance and composition for protecting nerve cells
  • Cordyceps cicadae/cordyceps sobolifera mycelium active substance and composition for protecting nerve cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) The source of the organism

[0035] The large cicadae (Cordycepscicadae) mycelium used in this embodiment can be purchased from the large cicadae (Cordycepscicadae) mycelium BCRCMU30106 (deposit date: November 25, 2013) deposited in the Food Industry Development Research Institute of Taiwan, China day); the used little cicada flower (Cordycepssobolifera) mycelium system can be purchased from the little cicada flower (Cordycepssobolifera) mycelium BCRC37801 (deposit date: March 12, 2010) deposited in Taiwan, China day), but the cicadae mycelium active substance described in the present invention is not limited to be obtained by this strain.

[0036] (2) Fermentation of organisms

[0037] The liquid culture of the active substance of the cicadae mycelium comprises inoculating the cicadae mycelium on a plate, using a potato dextrin medium (PotatoDextrosoAgar, PDA) at an appropriate temperature such as 15-35°C (preferably about 25°C) After 5 days to two weeks of culti...

Embodiment 2

[0044] Analysis of the neurotoxic injury and protection of nerve cells by the active substance of the cicadae mycelium described in Example 1.

[0045] 1. Antagonize MPP + NG108-15 cell line toxicity

[0046] Cell lines of NG108-15 were laid at a density of 2×10 4 cells / ml-well 96-well flat-bottomed tissue culture plate; after 24 hours, each cell is properly attached to it; finally, these cells are tested with a test sample (active substance of cicadae mycelium). The detection samples used dimethyl sulfoxide (DMSO) as a solvent, and were dissolved in DMSO at concentrations of 10, 20 and 40 mM, respectively. The concentration of DMSO in the medium (medium) was maintained at no more than 1 μl / ml, so as to ensure that it would not affect the growth of NG108-15 cells; + (1-methyl-4-phenylpyridine) treated NG108-15 cells; four days later, remove the medium and add 100 μl / well of MTT solution (0.5 mg / ml); put the cells in 37 ° C, 5 %CO 2 After culturing in the incubator for 1 h...

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Abstract

The invention discloses cordyceps cicadae / cordyceps sobolifera mycelium active substance and a composition for protecting nerve cells. Under the noncytotoxic concentration of 40 mu / ml, the cordyceps cicadae / cordyceps sobolifera mycelium active substance can be used for protecting the neurotoxicity of MPP+ to the dopaminergic neuron, and also can be used for inhibiting the cell apoptosis caused by the pressure of the endoplasmic reticulum caused by tunicamycin, and therefore, the cordyceps cicadae / cordyceps sobolifera mycelium active substance has the potential of preventing neurodegenerative diseases.

Description

technical field [0001] The invention relates to mycelium active substances, in particular to cicada flower mycelium active substances and a composition for protecting nerve cells. Background technique [0002] Neurodegenerative Diseases: [0003] Neurodegenerative diseases are symptoms in which neurons in the brain and central nervous system gradually lose function. The brain and spinal cord are composed of various neurons, each responsible for different functions, such as controlling movement, processing and receiving information, and making judgments. The hippocampus in the brain is mainly the hub responsible for learning and memory. Damage to nerve cells in the hippocampus can lead to dementia and learning defects. Neurodegenerative diseases can result from the deterioration of neurons or the loss of function of the myelin sheath that transmits messages over time. Because the nerve cells in the brain and spinal cord are not easy to regenerate, as long as they are damag...

Claims

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Application Information

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IPC IPC(8): A61K36/066A61P25/28A61K35/64
Inventor 陈劲初许胜杰陈彦博叶淑幸
Owner GRAPE KING
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