The embodiment of the invention discloses a novel anti-theft anti-looseness bolt and nut locking device. The device comprises a bolt, an anti-looseness nut is connected to the side wall of the bolt ina screwed mode, a locking mechanism is arranged at the lower end of the anti-looseness nut, a lock bolt hole is formed in the position, corresponding to the locking mechanism, of the side wall of thelower end of the bolt, and a locking assembly for limiting the locking mechanism is arranged in the lock bolt hole. According to the novel anti-theft anti-looseness bolt and nut locking device, the locking assembly is arranged, the locking assembly can be used in a mode of cooperating with the locking mechanism, when the anti-looseness nut is screwed on the bolt, a lock bolt body on the locking assembly can be embedded into the tooth surface to form a locking structure, therefore, the phenomenon that the bolt is easily loosen due to strong impact cannot occur, and then the situation that a large amount of time is consumed for maintenance is avoided; and meanwhile, when the locking structure is formed, the lock bolt body is always embedded into the tooth surface so that reverse rotation cannot occur to the locking nut again, then the anti-theft effect is achieved, the structure is compact, the flexibility is high, and mounting is convenient.