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Artificial cultivation method of cordyceps sobolifera

A technology of artificial cultivation and cicada flower, applied in the directions of botanical equipment and methods, cultivation, plant cultivation, etc., can solve the problems of control, unfavorable appearance and quality of sporophore bundles, etc., and achieve the effect of improving yield

Inactive Publication Date: 2017-07-07
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the light sources used in the artificial cultivation of cicadas in the light culture stage are white composite light, which is not conducive to the control of the appearance and quality of spore bundles.

Method used

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  • Artificial cultivation method of cordyceps sobolifera

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Experimental program
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Effect test

Embodiment 1

[0023] The expanded cultivation of embodiment 1 cicadae bacterial classification

[0024] Slant cultivation: Inoculate cicadae strains into inclined test tubes, then put the inclined test tubes inoculated into 22°C incubators, and cultivate for 7 days until the mycelium is full of test tubes;

[0025] Shake flask culture: put 200m1 liquid culture medium in a 500m1 Erlenmeyer flask, autoclave for 30 minutes under a pressure of 0.11Mpa, wait to cool to room temperature, inoculate the strains of 1 inclined test tube on the 500m1 Erlenmeyer flask culture medium, and Place in a constant temperature shaking incubator at a temperature of 22±1°C and culture at 150 rpm for 3 days;

[0026] Seed tank culture: put 20L liquid medium in a 50L air-lift fermenter, the temperature of the medium is higher than 95°C, add edible defoamer, the amount added is 0.03% of the weight of the medium, and under the pressure of 0.11Mpa, feed Heat the steam to 121°C, and keep the pressure for 30-45 minute...

Embodiment 2

[0030] The expanded cultivation of embodiment 2 cicadae bacterial classification

[0031] Culture process and culture condition are basically the same as embodiment 1, difference is:

[0032] Inclined test tube medium: 10g yeast extract powder, 40g glucose, 10g peptone, 20g agar, the remaining water to 1000ml, pH 6.0;

[0033] Liquid medium in shake flasks, seed tanks and fermenters: 40g of bran juice, 30g of white granulated sugar, the remaining water to 1000 milliliters, pH 6.5.

Embodiment 3

[0034] The expanded cultivation of embodiment 3 cicadae bacterial classification

[0035] Culture process and culture condition are basically the same as embodiment 1, difference is:

[0036] Inclined test tube medium: 40g maltose, 10g peptone, 20g agar, and make up the rest to 1000ml;

[0037] Liquid culture medium in shake flasks, seed tanks and fermentation tanks: 30g yeast extract powder, 30g white granulated sugar, 5g soybean hydrolyzed protein, and make up the rest to 1000ml with water, and the pH value is 6.5.

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Abstract

The invention relates to an artificial cultivation method of cordyceps sobolifera. The method comprises the following steps: step 1, expanded cultivation of cordyceps sobolifera strains; step 2, soil culture of strains obtained by expanded cultivation in the step 2, step 3, harvesting of coremium. In the step, the soil culture comprises dark culture and light culture phases. In the light culture, green light is utilized for irradiation. The method has the following beneficial effects: in the phase of cordyceps sobolifera cultivation, green light (single-color light) is utilized for irradiation; compared with white composite light, the yield of coremium is greatly increased.

Description

technical field [0001] The invention relates to an artificial cultivation method of cicada flowers, in particular to an artificial cultivation method for improving the quality of spore bundles of cicadas by carrying out light cultivation with monochromatic light. Background technique [0002] The traditional Chinese medicine cicada is a complex of bacteria and insects formed by Isaria cicadae Miquel (Paecilomycescicadae (Miquel) Samson) parasitizing on cicada nymphs. It is one of the famous traditional Chinese medicinal materials in my country. Its medicinal use is earlier than that of Cordyceps sinensis 800 years. Cicada is a medicinal and edible fungus with high nutritional value and specific efficacy. Cicada can produce a variety of physiologically active substances that play an important role in medical treatment and health care, including nucleosides, cyclic peptides, polysaccharides, alcohols, sterols and organic acids, etc., in regulating immunity, improving kidney fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 孙长胜路文遥王玉芹纪伟张忠亮施伟勤顾玲玲谭悠久彭国杰闫文娟
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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