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Method for artificially culturing paecilomyces cicadae and application of culturing product thereof

A technology of artificial cultivation of Paecilomyces cicadae, applied in the direction of microorganism-based methods, applications, biochemical equipment and methods, etc., which can solve the problems of high cost and long cultivation period

Active Publication Date: 2011-11-16
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, the method of artificially cultivating Paecilomyces cicadae often has the problem of relatively long cultivation period and high cost, and Paecilomyces cicadae has quasi-sexual reproduction, so strain optimization is the key to production

Method used

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  • Method for artificially culturing paecilomyces cicadae and application of culturing product thereof
  • Method for artificially culturing paecilomyces cicadae and application of culturing product thereof
  • Method for artificially culturing paecilomyces cicadae and application of culturing product thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 1. Preparation and cultivation of strains:

[0061] (1) Strain preparation: prepare potato sucrose agar medium or potato sucrose culture solution for subsequent use;

[0062] ①Preparation of slant seeds: wild cicadas collected from the source of Sanjiang in Yunnan Province were obtained through tissue separation and purification, and after transferring to tubes, they were cultured at 22°C for 5 days, and selected for future use;

[0063] ②Preparation of the primary seed: put the potato sucrose culture solution into the Erlenmeyer flask, put it in a sterilizing pot and sterilize it under the pressure of 0.15MPa for 30min, and put the spare slanted seed into the Erlenmeyer flask under aseptic condition after cooling , cultivated for 5 days at 22°C with an oscillation frequency of 130r / min, until a large number of mycelium balls appeared in the Erlenmeyer flask and then used for later use;

[0064] The ratio of the PSA medium used in this embodiment is per 1000ml of mediu...

Embodiment 2

[0076] 1. Preparation and cultivation of strains:

[0077] (1) Strain preparation: prepare potato sucrose agar medium or potato sucrose culture solution for subsequent use;

[0078] ①Preparation of slant seeds: wild cicadae collected from the source of Sanjiang in Yunnan Province was obtained through tissue separation and purification, and after transferring to tubes, cultured at 25°C for 7 days, and selected for future use;

[0079] ②Preparation of the primary species: put the potato sucrose culture solution into a conical flask, sterilize it in a sterilizing pot for 30 minutes under a pressure of 0.15MPa, put it into a conical flask after cooling, and place it at 25°C with an oscillation frequency of 150r / Cultivate for 3 days under the condition of min, until a large number of mycelium balls appear in the Erlenmeyer flask for later use;

[0080] The ratio of the PSA medium used in this embodiment is per 1000ml of medium: 200g of potatoes, 20g of sucrose, and 20g of agar. ...

Embodiment 3

[0094] 1. Preparation and cultivation of strains:

[0095] (1) Strain preparation: prepare potato sucrose agar medium or potato sucrose culture solution for subsequent use;

[0096] ①Preparation of slant seeds: wild cicadas collected from the source of Sanjiang in Yunnan Province were obtained through tissue separation and purification, and after transferring to tubes, they were cultured at 22°C for 5 days, and selected for future use;

[0097] ②Preparation of the primary species: put the potato sucrose culture solution into a conical flask, put it in a sterilizer for 30 minutes under a pressure of 0.15 MPa, put it into a conical flask after cooling, and place it at 23°C with an oscillation frequency of 130r / Cultivate under the condition of 5 min for 5 days, until a large number of mycelium balls appear in the Erlenmeyer flask for subsequent use;

[0098] The ratio of the potato sucrose agar medium used in this embodiment is per 1000ml of medium: 200g of potatoes, 20g of suc...

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Abstract

The invention discloses a method for artificially culturing paecilomyces cicadae and application of a culturing product thereof. The method for artificially culturing paecilomyces cicadae in large scales comprises the steps of: preparing strains, dosing and packing into a box, sterilizing, inoculating, solid-fermenting, collecting and the like. The paecilomyces cicadae is cultured by utilizing grains, such as corn flour, bran, wheat, barley, rice, millet, broomcorn and the like and culture mediums of bagasse, cane sugar, shell powder, silkworm chrysalis meal and potassium nitrate. The raw materials are obtained from local resources, a large amount of production cost is saved, the culturing period is shortened, and cordyceps sobolifera obtained by culturing has the advantages of high quality and favorable stability and the like. A culture obtained by the invention can be used for preparing foods, health-care products, drugs and cosmetics with functions of fighting tumor, regulating immunity, reducing blood sugar, blood fat and blood pressure, improving eye sight, resisting radiation, dispelling heat and easing pains, calming and hypnotizing, nourishing and strengthening, improving kidney function and the like.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of medicinal fungi, and in particular relates to a method for artificial cultivation of Paecilomyces cicadae and an application of the culture product. Background technique [0002] Cicada flower, scientific name Paecilomyces cicadae, is the product of certain cicada nymphs parasitized by Paecilomyces cicadae, and is a complex of fungi and insects. This fungus was named by Miquel in 1838 as Isaria cicadae. Since then, there have been many synonyms of the same thing. Such as Cordyceps cicadae, Isaria basili, Sphaeria sinclairii, Torrubia caespitosa, Cordyceps sinclairii, Isaria hariottii, Cordyceps sobolifera, Isaria sinclairii, Isaria mokanshawii, Isaria arbuscu / a, etc. Medicine thinks that cicadae is a kind of precious medicinal material similar to Cordyceps sinensis. [0003] Some scholars believe that the sexual stage of Paecilomyces cicada is Cordyceps cicadae. Cicada grand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L1/28A23K1/16A61K36/06A61K8/99A61P35/00A61P37/02A61P3/06A61P3/10A61P9/12A61P27/02A61P17/16A61P29/00A61P25/20A61P13/12A61P1/14A61Q1/00C12R1/79A23K20/00A23L31/00
Inventor 陈祝安孙长胜赵伟李成
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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