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Method for cultivating calcium-rich cordyceps sobolifera

A cultivation method and technology of calcium-enriched cicadas, which are applied in botany equipment and methods, horticulture, applications, etc., can solve the undisclosed problems of adding calcium salts to cultivate calcium-enriched cicadas

Active Publication Date: 2016-10-05
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art does not disclose the technology of adding calcium salts to cultivate calcium-rich cicadas in the cicada culture medium

Method used

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  • Method for cultivating calcium-rich cordyceps sobolifera
  • Method for cultivating calcium-rich cordyceps sobolifera
  • Method for cultivating calcium-rich cordyceps sobolifera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 cicadae bacterial classification cultivation method

[0063] Inclined surface cultivation: inoculate the cicadae bacterial classification (embodiment 10) in the inclined test tube, then put the inclined test tube inoculated with the bacterial classification into a 22°C incubator, and the cultivation time is 7 days, until the mycelia are covered with the test tube;

[0064] Liquid culture: put 200m1 liquid culture medium in a 500m1 Erlenmeyer flask, autoclave for 30 minutes under the pressure of 0.11Mpa, wait to cool to room temperature, inoculate the strains of 1 inclined test tube on the culture medium of 500m1 Erlenmeyer flask, and place Cultivate in a constant temperature shaking incubator at a temperature of 22±1°C and 150 rpm for 3 days;

[0065] Solid culture: Inoculate the strains obtained from seed culture with 25% of the inoculation amount, and put the inoculated container into the culture room for culture. The temperature of the culture room is 22...

Embodiment 2

[0070] Incline culture: inoculate cicadae strains (Paecilomyces cicadae (Miq.) Samson] preservation number is CGMCC No.3453) in inclined test tubes, and then put the inoculated inclined test tubes into 22°C for cultivation box, the culture time is 7 days, until the mycelium is full of test tubes;

[0071] Liquid culture: put 200m1 liquid culture medium in a 500m1 Erlenmeyer flask, autoclave for 30 minutes under the pressure of 0.11Mpa, wait to cool to room temperature, inoculate the strains of 1 inclined test tube on the culture medium of 500m1 Erlenmeyer flask, and place Cultivate in a constant temperature shaking incubator at a temperature of 22±1°C and 150 rpm for 3 days;

[0072] Solid culture: Inoculate the strains obtained from seed culture with 25% of the inoculation amount, and put the inoculated container into the culture room for culture. The temperature of the culture room is 22-24°C, and the relative air humidity is 65%-70%. Shading, so that the germ chamber is ba...

Embodiment 3

[0077] According to the method of Example 1, solid medium: corn medium, the addition of citric acid-calcium malate is 0.25%, and an appropriate amount of water is mixed. Method is with embodiment 1, and result is as follows:

[0078] Table 6 Calcium-enriching effect of citric acid-calcium malate on corn medium

[0079]

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Abstract

The invention relates to a method for cultivating calcium-rich cordyceps sobolifera. The method comprises the followings steps that 1, a cordyceps sobolifera strain is subjected to expansion cultivation in a liquid medium; 2, a solid medium is inoculated with the strain amplified in step 1 for solid culture; 3, coremium is harvested. The method is characterized in that calcium salt is added into the liquid medium in step 1 and / or the solid medium in step 2, and the calcium salt is any one or more of calcium citrate, citric acid-calcium malate, calcium acetate, calcium gluconate and calcium lactate.

Description

technical field [0001] The invention relates to a method for cultivating cicadas, in particular to a method for cultivating calcium-rich cicadas. Background technique [0002] Cicada (Isaria cicadae Miquel) belongs to Ascomycot, Pezizomycotina, Sordariomycetes, Hypocreales, Cordycipitaceae of Fungi (FUNGI). , Isaria (Isaria), is my country's famous and precious Chinese medicinal materials, is a kind of Cordyceps fungus that parasitizes on cicadas (commonly known as "cicadas"). Its medicinal use has a history of more than 1,000 years. It is one of the traditional precious medicinal materials in my country and has various medicinal values. The main ingredients are adenosine, cordyceps polysaccharide, cordycepic acid (mannitol), cordycepin, uracil, sterols, alkaloids, vitamins, inorganic salts, mineral elements, etc. Cicada has unique and extensive effects, such as improving kidney function, anti-tumor, regulating immune system, nourishing and strengthening, improving cell abi...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 纪伟董琼陆小芳陈奇超彭国杰胡建峰张忠亮徐煜施伟勤陆文遥孙长胜
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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