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Anti-oxidative magnetic iron oxide nanoparticles and preparation method thereof

A magnetic iron oxide and nanoparticle technology, applied in the field of materials science, can solve the problems of mediating target organ oxidative stress damage, imbalance of oxidation and anti-oxidation systems, and aggravate negative remodeling of target organs, so as to reduce oxidative stress Toxicity, improvement of myocardial histocompatibility, effect of improvement of biocompatibility

Inactive Publication Date: 2019-05-17
SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the above-mentioned technical problems in the prior art, the present invention provides an anti-oxidation magnetic iron oxide nanoparticle and a preparation method thereof. The anti-oxidation magnetic iron oxide nanoparticle and the preparation method thereof are to solve the problem of synthesis of the prior art. The time- and concentration-dependent cytotoxicity of MNPs can easily cause chronic iron overload in myocardial tissue, lead to an imbalance in oxidation and antioxidant systems, mediate oxidative stress damage in target organs, induce apoptosis, and aggravate the negative remodeling of target organs , technical issues that lead to functional deterioration

Method used

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  • Anti-oxidative magnetic iron oxide nanoparticles and preparation method thereof
  • Anti-oxidative magnetic iron oxide nanoparticles and preparation method thereof
  • Anti-oxidative magnetic iron oxide nanoparticles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Prepare antioxidant N-acetylcysteine ​​(NAC) standard solution

[0028] Weigh 50 mg of NAC into a 100 mL beaker, add 20 mL of chloroform to ultrasonically dissolve, and transfer to a 100 mL volumetric flask after dissolution. Wash the beaker 3 times with chloroform, and pour the washed chloroform into a volumetric flask, add chloroform to the volumetric flask to the mark to obtain solution A, the concentration of which is 0.5 mg / mL. Prepare standard solutions of different concentrations: B: 0.1 mg / mL; C: 0.02 mg / mL; D: 0.004 mg / mL; E: 0.0008 mg / mL; F: 0.00016 mg / mL; G: 0.000032 mg / mL.

Embodiment 2

[0030] Core-shell structured magnetic mesoporous silica nanoparticles (M-MSNs) loaded with NAC

[0031] Take 0.410mL of M-MSNs solution in a 2mL centrifuge tube (4 parts, 2mg each), the concentration of M-MSNs solution is 4.8mg / mL, centrifuge at 12800rpm for 25min, after centrifugation, remove the supernatant; Add 1.5mL 0.5mg / mL NAC to each centrifuge tube, ultrasonic for 2min; after ultrasonic, seal it with a parafilm and place it in a shaker (25℃, 250rpm) for 2h; after shaking, take out the sample and centrifuge, and centrifuge for 30min at 12800rpm. Pour the supernatant into a centrifuge tube, dry the precipitate in a vacuum drying oven at 70°C, and subject the dried product to thermogravimetric analysis (TGA); add 1mL of the supernatant to 4mL of chloroform, shake well to obtain the supernatant The concentration of the solution is 0.5 mg / mL, and UV-Vis is tested.

[0032] Through characterization analysis, NAC was successfully loaded onto M-MSNs in the chloroform phase.

[0033...

Embodiment 3

[0035] Example 3 M-MSN@NAC reduces the short-term antioxidant effect of MNP-mediated hypoxia-reoxygenation myocardial cytotoxicity in vitro

[0036] The SD rat neonatal rat cardiomyocytes were separated by tissue block digestion and identified; neonatal rat cardiomyocytes were induced to hypoxia by a hypoxia box for 3 hours, and then returned to the incubator (culture condition 95% O 2 And 5% CO 2 ) Medium reoxygenation for 3 hours to establish hypoxia-reoxygenation (H / R) cardiomyocyte model, then MNPs, M-MSNs and M-MSN@NAC were co-cultured with H / R cardiomyocytes for 24 hours and then oxidative stress was detected , Mitochondrial membrane potential, cell damage and apoptosis indicators.

[0037] Compared with H / R cardiomyocytes, DHE test showed that ROS generation in MNP group and M-MSN group was significantly increased ( image 3 ), leading to an imbalance in the cellular oxidation and antioxidant systems; ELASA and biochemical tests suggest that the lipid peroxidation products MD...

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Abstract

The invention discloses anti-oxidative magnetic iron oxide nanoparticles which comprise magnetic mesoporous silicon dioxide nanoparticles with a core-shell structure formed by coating the surface of an Fe3O4 inner core with mesoporous silicon dioxide, and also comprise an antioxidant N-acetylcysteine, wherein the antioxidant N-acetylcysteine is supported in mesoporous silicon dioxide. The invention also provides a preparation method of the anti-oxidative magnetic iron oxide nanoparticles. The method comprises the steps of preparing an antioxidant N-acetylcysteine solution; weighing a magneticmesoporous silicon dioxide nanoparticle solution with the core-shell structure, executing centrifugation, then adding the antioxidant N-acetylcysteine solution; sealing with a sealing film after ultrasonic treatment, placing the solution into a shaking table to shake, taking out a sample after completion, and centrifuging and drying the sample to obtain the anti-oxidative magnetic iron oxide nanoparticles. After the anti-oxidative magnetic iron oxide nanoparticles reach a target part, the antioxidant is released, so that the oxidative stress damage mediated by the iron oxide is relieved, and the histocompatibility of the magnetic iron oxide nanoparticles is improved.

Description

Technical field [0001] The invention belongs to the field of materials science, and relates to a nano material, specifically an anti-oxidation magnetic iron oxide nano particle and a preparation method thereof. Background technique [0002] Magnetic iron oxide nanoparticles (MNPs) are an emerging functional material with nanometer diameter and superparamagnetism, which are increasingly used in the cardiovascular field, including magnetic resonance imaging, cell tracing, drug carriers, genes and cell targets To wait for biomedical experiments and even clinical research. Therefore, MNPs have broad application prospects and huge clinical transformation potential in the diagnosis and treatment of cardiovascular diseases. [0003] However, in recent years, a large number of studies have confirmed that MNPs have time- and concentration-dependent cytotoxicity. When used in the cardiovascular field to diagnose or treat diseases, it is easy to cause chronic iron overload in myocardial tiss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/02A61K47/04A61K47/18A61K49/18C12Q1/02
Inventor 沈运丽胡凤麟黄浙勇
Owner SHANGHAI EAST HOSPITAL EAST HOSPITAL TONGJI UNIV SCHOOL OF MEDICINE
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