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Support material with conductivity and antioxygenic property and preparation method thereof

A technology of anti-oxidative properties and scaffold materials, applied in the direction of prosthesis, tissue regeneration, medical science, etc., can solve the problem of insufficient attention, and achieve the effect of obvious differentiation effect, promotion of differentiation, and good biocompatibility.

Active Publication Date: 2016-12-07
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the previous studies on scaffold materials for myocardial tissue engineering focused on improving the chemical, physical and processing properties of the materials, and insufficient attention was paid to the function of the myocardial infarction microenvironment in regulating ROS.

Method used

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  • Support material with conductivity and antioxygenic property and preparation method thereof
  • Support material with conductivity and antioxygenic property and preparation method thereof
  • Support material with conductivity and antioxygenic property and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Synthesis of aniline pentamer-glutathione complex with both electrical conductivity and antioxidant properties

[0031] (1) Add 9.21g of N-phenyl-1,4-p-phenylenediamine and 5.00g of succinic anhydride to 300ml of dichloromethane and stir for 12h. Filter and wash the precipitate with ether until the filtrate is colorless. Dry in a vacuum oven for 12-48 hours to obtain N-((4-anilino)phenyl)butyramide, which is ready for use.

[0032] (2) 13.6g of N-((4-anilino)phenyl)butanamide and 2.60g of p-phenylenediamine obtained in step (1) were dissolved in 72mL of DMF, and after stirring in an ice bath for 2 hours, pre- Cold 288.39 mL of mixed solution (containing 144.19 mL of DMF, 120.16 mL of distilled water and 20.04 mL of concentrated hydrochloric acid).

[0033] (3) Dissolve 10.96g of ammonium persulfate in 240.31mL of hydrochloric acid solution (1mol / L), and slowly add it to the mixed solution obtained in step (2), stir it rapidly for 1-2h, then add the solutio...

Embodiment 2

[0039] Example 2 Preparation of gelatin / aniline pentamer-glutathione porous scaffold with both electrical conductivity and antioxidant properties

[0040] (1) A certain amount of gelatin is dissolved in dimethyl sulfoxide (DMSO), and a DMSO solution with a mass concentration of 8% gelatin is prepared for later use.

[0041] (2) Weigh and weigh 15mg, 30mg, 60mg and 120mg of the AP-GSH complex and add them to the 3ml gelatin solution prepared in step (1), so that the percentage of AP-GSH in gelatin is 6.25%, 12.5%, 25% % and 50%, stirred for 2-3 hours to form a uniform and stable solution for later use.

[0042] (3) Add 600 μL of 0.9% glutaraldehyde aqueous solution to the solution obtained in step (2), mix it quickly, pour it into a suitable mold at room temperature, and let it stand still to become a gel.

[0043] (3) After standing still for 12 hours, take out the gel and soak it in water for 3 days, changing the water 3 times a day. Then put the gel into the prepared 5% so...

Embodiment 3

[0045] Example 3 Preparation of gelatin / aniline pentamer porous scaffold

[0046] (1) A certain amount of gelatin is dissolved in dimethyl sulfoxide (DMSO), and a DMSO solution with a mass concentration of 8% gelatin is prepared for later use.

[0047] (2) Weigh and weigh 30 mg of the CCAP complex and add them to the 3 ml gelatin solution prepared in step (1), so that the percentage of CCAP in gelatin is 12.5%, stir for 2-3 hours to form a uniform and stable solution, and set aside.

[0048] (3) Add 600 μL of 0.9% glutaraldehyde aqueous solution to the solution obtained in step (2), mix it quickly, pour it into a suitable mold at room temperature, and let it stand still to become a gel.

[0049] (3) After standing still for 12 hours, take out the gel and soak it in water for 3 days, changing the water 3 times a day. Then soak the gel in the prepared 5% sodium cyanoborohydride aqueous solution for 4 hours to remove unreacted glutaraldehyde, soak in water for 24 hours to fully ...

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Abstract

The invention discloses a support material with conductivity and an antioxygenic property and a preparation method thereof. The support material is prepared from phenylamine pentamer modified by glutathione, and gelatin. The support material has favorable mechanical strength and adjustable degradation characteristic. More importantly, the support material has good conductivity, and can effectively scavenge various oxygen radicals such as hydroxyl radicals, organic oxygen radicals and superoxide radicals. According to the support material with the conductivity and the antioxygenic property and the preparation method thereof provided by the invention, the dual-network conductive support material is prepared by adopting one-step shaping, so that the preparation method is simple, components are uniform and controllable. More importantly, the support material has favorable biocompatibility and can support the adhesion and the growth of various cells. Stem cells can be promoted to differentiate into cardiac muscle cells, so that the support material has potential application prospect in cardiac muscle tissue engineering.

Description

technical field [0001] The invention belongs to the preparation technology of tissue engineering biomaterials, and in particular relates to a scaffold material with both electrical conductivity and anti-oxidation properties and a preparation method thereof. Background technique [0002] Myocardial infarction is one of the major diseases that endanger human health and life. The five-year survival rate of patients diagnosed with myocardial infarction is only 50%. Traditional treatment methods cannot fundamentally repair damaged myocardium. Recent studies have shown that myocardial tissue engineering based on biomaterials and seed cells has potential application prospects in the treatment of myocardial infarction. Among them, biomaterials play a very critical role in providing the necessary mechanical support for the repair of damaged myocardium, and providing a suitable microenvironment for the survival and proliferation of transplantation and host cells. Myocardium is an el...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/52C08J3/075
CPCA61L27/22A61L27/222A61L27/52A61L27/56A61L2430/20C08J3/075C08J2389/00C08L89/00
Inventor 李俊杰王常勇房万才郝彤
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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