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Hydrogel microcapsule for encapsulating nicotinic acid metal organic framework and preparation method and application thereof

A metal-organic framework and microcapsule technology, applied in the field of medical materials, can solve the problems of limited application of biocompatibility, irregular drug release, random and uncontrollable, etc., to reduce oxidative stress damage, promote wound repair, and promote healing Effect

Inactive Publication Date: 2020-04-14
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the encapsulation of metal-organic frameworks in hydrogels is random and uncontrollable, which makes the drug release irregular and uncontrollable
Furthermore, the biocompatibility of organic ligands greatly limits their applications in biomedicine

Method used

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  • Hydrogel microcapsule for encapsulating nicotinic acid metal organic framework and preparation method and application thereof
  • Hydrogel microcapsule for encapsulating nicotinic acid metal organic framework and preparation method and application thereof
  • Hydrogel microcapsule for encapsulating nicotinic acid metal organic framework and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Assembly of Microfluidic Device

[0038] (1) Preparation of the capillary: select the capillary drawn by the straight spray gun, such as figure 1 The capillary with a spindle-shaped outlet shown in (a) is used as two internal phase pipes, the capillary with an opening of 40-60 μm is marked as tube I, and the capillary with a mouth of 100 μm is marked as tube II; the choice is drawn from the capillary instrument drawn as figure 1 The capillary with tapered outlet shown in (b) is used as the intermediate phase pipeline, which is recorded as tube III; the cylindrical capillary tube with an inner diameter of 580 μm and an outer diameter of 1 mm is selected as the external phase pipeline, and is recorded as tube IV; choose a suitable length, and the length of the inner edge is The 1.05mm square capillary is the support pipe at the junction of the inner phase pipe and the outer phase pipe, which is convenient for follow-up observation.

[0039] Grind tube III with sandpa...

Embodiment 2

[0045] Embodiment 2 sustained release effect

[0046] Since the organic ligands of copper nicotinate and zinc nicotinate are nicotinic acid, the release amount of metal organic framework compounds can be reflected by measuring the release amount of nicotinic acid. Specific steps: Taking the nicotinic acid-copper-zinc metal organic framework prepared in Comparative Example 1 as a control, soak the washed microcapsules (prepared in Example 1) in 1 mL of PBS buffer solution, place them on a shaker, and set the temperature at 37°C , speed 300rpm. Take out 100 μL of extract at specific time and supplement 100 μL of 37°C fresh PBS solution. After diluting the extracted extract 10 times, measure the value of absorbance with a UV spectrophotometer at a wavelength of 263 nm. see results image 3 ,Depend on image 3 It can be found that under the wrapping of calcium alginate gel, the release rate of niacin is significantly slowed down, and there is no burst release phenomenon, indic...

Embodiment 3

[0047] Example 3 Biocompatibility

[0048] Add 100 μL of NIH 3T3 cell suspension to each well of a sterilized 96-well plate, about 4000 cells per well, and culture in a cell culture incubator for 24 hours until the cells grow adherently. At the same time, the microcapsules (prepared in Example 1) produced by microfluidics and wrapped with different amounts of metal-organic framework compounds were respectively placed in orifice plates containing 1 mL of culture medium for 48 hours. Then the microcapsules were taken out, and 1 mL of fresh medium was added to the extract in each well. NIH 3T3 cells were resuspended, and the cell suspension was added to the mixture of extract and fresh medium for 24 hours.

[0049] Immunofluorescent staining of cells in the well plate, the specific steps are as follows:

[0050] (1) Add 10 μL of 1 mg / mL Calcein-AM solution and 15 μL of 1 mg / mL PI solution to 5 ml of PBS buffer to prepare a fluorescent staining solution.

[0051] (2) Aspirate t...

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Abstract

The invention discloses a hydrogel microcapsule for encapsulating a nicotinic acid metal organic framework and a preparation method and application thereof. The preparation method of the hydrogel microcapsule for encapsulating the nicotinic acid metal organic framework comprises the steps that a nicotinic acid solution is used as an inner phase 1; a mixed solution of a zinc acetate solution and acopper acetate solution is used as an inner phase 2; a sodium alginate solution is used as an outer phase; and a microfluidic device is used for spraying mixed liquid drops of the inner phase 1 and the inner phase 2 wrapped by the outer phase into a calcium chloride solution to prepare the hydrogel microcapsule for encapsulating the nicotinic acid metal organic framework. The hydrogel microcapsulefor encapsulating the nicotinic acid metal organic framework, which is prepared by the invention, has ideal controlled release effect, antibacterial effect and anti-oxidative stress effect, and can be used for preparing wound healing materials or medical instruments.

Description

technical field [0001] The invention belongs to the field of medical materials, and relates to a hydrogel microcapsule encapsulating a nicotinic acid metal organic framework, a preparation method and an application thereof. Background technique [0002] 1. Wound repair problems [0003] With the rapid development of social economy, the number of patients with wounds caused by various diseases is increasing, especially the refractory wounds caused by burns, trauma, postoperative wound infection, etc. are increasing year by year. The patient suffers a lot physically and mentally, and the cost of treatment is getting higher and higher. According to statistics, about 1% of the world's population is plagued by persistent wound problems, and about 5% of medical expenses are spent on wound repair. The wounds caused by these diseases are of many types, involve a wide range of specialties, have complex mechanisms, and are difficult to treat. [0004] 2. Current status of wound hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/30A61K33/34A61K31/455A61K9/52A61K47/36A61P17/02A61L15/44
CPCA61K9/5036A61K31/455A61K33/30A61K33/34A61L15/44A61L2300/102A61L2300/602A61L2300/622A61P17/02A61K2300/00
Inventor 任建安陈灿文吴秀文王革非顾国胜陈国璞
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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