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Application of lycopene in preparation of human semen cryoprotectant

A technology of cryoprotectant and lycopene, applied in application, preservation of human or animal bodies, animal husbandry, etc., can solve problems such as sperm damage, cell apoptosis, and reduced sperm fertilization ability

Inactive Publication Date: 2015-04-29
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Often results in direct damage to the sperm, and in addition
Reactive oxygen species and their metabolites can also be toxic to cells, causing apoptosis and directly causing sperm damage
[0006] ③Protein damage: Since reactive oxygen species can induce protein thiol peroxidation, the function and structure of sperm will change, making sperm more susceptible to damage. In addition, the generation of a large amount of reactive oxygen species can also cause damage to the acrosome structure, thereby reducing spermatozoa. fertilization capacity

Method used

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  • Application of lycopene in preparation of human semen cryoprotectant
  • Application of lycopene in preparation of human semen cryoprotectant
  • Application of lycopene in preparation of human semen cryoprotectant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Each 100mL human semen cryoprotectant containing lycopene contains the following substances:

[0059] Glucose 1.5g, trisodium citrate dihydrate 1.3g, glycerin 20ml, lycopene 4.296×10 -4 g, egg yolk 20ml, dimethyl sulfoxide 0.01mL, and double distilled water as the rest;

[0060] The preparation method of the human semen cryoprotectant of described lycopene comprises the following steps:

[0061] (1) Preparation of 99.99mL egg yolk and glycerin composite cryoprotectant

[0062] (1) Weigh 1.5g glucose and 1.3g trisodium citrate dihydrate, add double distilled water to 59.99mL;

[0063] (2) After adding 20mL glycerin, mix completely, and filter with a 0.45μm microporous filter after completely dissolving;

[0064] (3) Add 20 mL of fresh egg yolk to make a suspension; fresh egg yolk preparation: wash the eggs, remove the shell, puncture the egg membrane, and suck the egg yolk with a syringe;

[0065] (4) Place the prepared suspension in a 56°C water bath for 30 minutes,...

Embodiment 2

[0082] Each 100mL human semen cryoprotectant containing lycopene contains the following substances:

[0083] Glucose 1.5g, trisodium citrate dihydrate 1.3g, glycerin 20ml, lycopene 1.074×10 -3 g, egg yolk 20ml, dimethyl sulfoxide 0.025mL, the balance is double distilled water;

[0084] The preparation method of the human semen cryoprotectant of described lycopene comprises the following steps:

[0085] (1) Preparation of 99.975mL egg yolk and glycerin composite cryoprotectant

[0086] (1) Weigh 1.5g glucose and 1.3g trisodium citrate dihydrate, add double distilled water to 59.975mL;

[0087] (2) After adding 20mL glycerin, mix completely, and filter with a 0.45μm microporous filter after completely dissolving;

[0088] (3) Add 20 mL of fresh egg yolk to make a suspension; fresh egg yolk preparation: wash the eggs, remove the shell, puncture the egg membrane, and suck the egg yolk with a syringe;

[0089] (4) Place the prepared suspension in a 56°C water bath for 30 minute...

Embodiment 3

[0106] Each 100mL human semen cryoprotectant containing lycopene contains the following substances:

[0107] Glucose 1.5g, trisodium citrate dihydrate 1.3g, glycerin 20ml, lycopene 2.148×10 -3 g, egg yolk 20ml, dimethyl sulfoxide 0.05mL, the rest is double distilled water;

[0108] The preparation method of the human semen cryoprotectant of described lycopene comprises the following steps:

[0109] (1) Preparation of 99.95mL egg yolk and glycerin composite cryoprotectant

[0110] (1) Weigh 1.5g glucose and 1.3g trisodium citrate dihydrate, add double distilled water to 59.95mL;

[0111] (2) After adding 20mL glycerin, mix completely, and filter with a 0.45μm microporous filter after completely dissolving;

[0112] (3) Add 20 mL of fresh egg yolk to make a suspension; fresh egg yolk preparation: wash the eggs, remove the shell, puncture the egg membrane, and suck the egg yolk with a syringe;

[0113] (4) Place the prepared suspension in a 56°C water bath for 30 minutes, sha...

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PUM

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Abstract

The invention relates to an application of lycopene in preparation of a human semen cryoprotectant, belonging to the field of human semen cryoprotectants. The invention discloses an application of lycopene in the preparation of the human semen cryoprotectant. Every 100mL of the lycopene-containing human semen cryoprotectant comprises the following materials: 1.5g of glucose, 1.3g of trisodium citrate dehydrate, 20ml of glycerin, 4.296*10<-4>-4.296*10<-3>g of lycopene, 20ml of yolk, 0.01-0.1mL of dimethyl sulfoxide and the balance of double distilled water. A certain amount of lycopene is added into the cryoprotectant, so that the human semen cryopreservation effect is obviously improved, the functional parameters such as forward activity ratio and survival rate, of the thawed human semen are obviously higher than those in a control group in which the lycopene is not added, the normal morphology rate, DNA integrity rate and mitochondrial membrane potential are obviously higher than those in the control group, and the sperm apoptosis rate is obviously lower than that in the control group, so that the lycopene has high safety on human semen quality protection.

Description

technical field [0001] The invention belongs to the field of cryoprotectants for human semen. Background technique [0002] Sperm are specialized cells that carry genetic material. The semen has been processed technically, under the protection of cryoprotectant, after a certain cooling procedure, and stored in an ultra-low temperature state (in liquid nitrogen), the sperm will freeze in a glassy form, and there will be no dehydration of the protoplasm, and its original structure will be protected. Thawed sperm can still be recovered and rejuvenated. The metabolic activity of the sperm in the frozen state is significantly inhibited, and its life is in a static state, so as to achieve the purpose of long-term preservation. [0003] Sperm cryoprotectant is the most important substance in sperm freezing technology. At present, the most commonly used sperm cryopreservation agent is a compound freezing solution containing glycerin and egg yolk, which is composed of glycerin, eg...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 梁作文王洪亮李付彪刘凌云郭凯敏刘浩沈娜戴小凡徐胜旗
Owner JILIN UNIV
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