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216results about How to "Low assay cost" patented technology

Electrochemical screening and early diagnosing instrument for malignant tumor

InactiveCN1866018AEasy to useOvercoming the deficiency of tumor-associated antigen specificityMaterial electrochemical variablesAbnormal tissue growthAntigen
The disclosed electrochemical screening and early diagnostic device for malignant tumor comprises: an immune detection chip (1) connected to a time-resolution multichannel potentiostat (2) that connects to a data process and display system (3) by an interface. Wherein, the chip (1) comprises eight working electrodes fixed different antigen films and Ag wire (a), Ag / AgCl reference electrode (b), a carbon-pair electrode (c), and an insulation film (d). This invention needs low cost, has intelligence and fit to fast detection.
Owner:NANJING UNIV

Direct rapid measuring method capable of desorbing mash gas content with coal seam

The method comprises: sampling coal core from underground mining; at underground mining, measuring and calculating the gas loss quantity of coal core; at the ground, measuring and calculating the desorption of the coal mining gas; measuring and calculation the grind gas desorption of partial coal core; calculating the content of gas capable of being desorbed.
Owner:CHINA COAL TECH ENG GRP CHONGQING RES INST

Quantitative detecting method for the polyphenol content

The present invention discloses a quantitative detection method of tea-polyphenol content. Said method includes the following steps: preparing standard sample liquor and color-developing liquor of sample to be detected, then placing proper quantity of color-developing liquor into colorimetric cell on the image collector, collecting original image information; utilizing tea-polyphenol content determination software system based on computer chromaticity identification to read collected original image, comparing chromaticity value of standard sample liquor with known tea-polyphenol content and drawing standard curve, obtaining chromaticity information of sample liquor to be detected so as to obtain the tea-polyphenol content of detected sample liquor.
Owner:CHINA AGRI UNIV

Salvianolic acid extract fingerprint spectrum and content measurement method of related components

The invention relates to establishment of a salvianolic acid extract fingerprint spectrum and a content measurement method of related components. The content measurement method includes the following steps: 1) establishing the salvianolic acid extract digitized quantitative fingerprint spectrum through ultrahigh performance liquid multi-wavelength visible ultraviolet detection method; 2) recognizing peaks in the fingerprint spectrum in the step 1) with mass spectrum to determine common fingerprint peaks of ultraviolet detection; and 3) performing measurement to contents of three indicative components, which are high in content, significant in activity and definite in structure, recognized in the step 2) through high performance liquid chromatography variable-wavelength detection method.
Owner:TIANJIN TASLY ZHIJIAO PHARMA

Rapid determination method for organophosphorus pesticide residue in vegetable and fruit

The invention refers to a kind of measuring method for organic phosphorus agricultural chemicals residues in garden stuff. The invention uses organic solvent to extract the organic phosphorus agricultural chemicals in fruits and vegetables, uses absorbing agent to eliminate the phosphorus extraction material of inorganic phosphorus agricultural chemicals in the extraction liquid, in odder to eliminate the disturbance. The invention uses air or nitrogen to blow off the solvent in the extraction liquid, adds in potassium sulfate solvent in the residual, decomposes, converts the organic phosphorus agriculture chemicals into orthophosphate, finally, uses molybdenum antimony splitting resisting luminosity method to measure the orthophosphate density in the decomposed liquid, thus works out the whole residue quantity of organic phosphorus agricultural chemicals in the fruits and vegetables. The speed is fast, it is accurate, stable, and reliable.
Owner:XIAMEN UNIV

Method for determining sinter mixture granularity

InactiveCN102443695AObjectively reflect the composition of particle sizeThe measurement result is objectiveParticle size analysisScreening proceduresGranularity
The invention provides a method for determining the sinter mixture granularity, which comprises the following steps of: (1) sample collection: sampling samples and determining the sample quantity; (2) sample preparation: reducing, dividing and freezing the samples; (3) screening: selecting screening equipment, determining the screening procedure, determining the screening time and carrying out screening; (4) weighing: weighing the weight of screened materials in all size grades; and (5) size grade calculation: calculating the percentage corresponding to all size grades according to a formula after the weighing completion of the samples in all size grades, recording the percentages, and calculating the average granularity. The method is characterized in that in the second step, the samples are frozen by a refrigerator after reduction and division. When the method is used, the problems that in the existing granularity determining method, the mixture size grade composition under the actual production condition cannot be objectively reflected, the mixed pelletization effect enhancing measure cannot be reasonably evaluated, and the like can be solved. The method has positive effects of effectively improving the proportioning, stabilizing the sinter process and stabilizing and improving the sinter mineral quality on sinter work procedures.
Owner:BAOSHAN IRON & STEEL CO LTD +1

Indoor simultaneous determination device and method of piping critical hydraulic gradient and particle wastage rate

InactiveCN103018424AEffective measurement of churn rateLow assay costEarth material testingPorosityEngineering
The invention discloses an indoor simultaneous determination device and method of piping critical hydraulic gradient and particle wastage rate. The determination device comprises a model cylinder for accommodating a sample, a sand-water collecting box for collecting water and soil particles gushed from the model cylinder, a downstream water collecting tank for separating the water and soil particles collected, and a data collecting system. The data collecting system is used to acquire a pressure change value of the model cylinder, a flow value of the downstream water collecting tank and a weight value of the soil particle gushed. According to the invention, the particle wastage rate is effectively determined in the soil piping process. The piping critical hydraulic gradient and the particle wastage rate can be determined, the determination cost is saved, the time required for determination is saved, and the efficiency is improved. The accuracy of the determination result is improved, the impact of initial porosity, hydraulic gradient, soil components and the like on the soil particle wastage rate in the piping process can be determined.
Owner:CHONGQING JIAOTONG UNIVERSITY

Lake eutrophication evaluation method based on water-body absorption coefficients

The invention discloses a lake eutrophication evaluation method based on water-body absorption coefficients. The lake eutrophication evaluation method includes the steps that the absorption coefficients of lake-water-body suspended particles and colored dissolved organic substances at a wave band of 400 nm are measured, and the sum of the absorption coefficients of the substances is calculated, and the water-body absorption coefficients at (440) of a lake water body at the wave band of 400 nm are obtained; after a relationship mode of the at (440) and lake comprehensive nutrition state coefficients TLI is established, the lake-nutrition-state classification standard based on the at (440) is determined according to the TLI coefficient range. Lake eutrophication is evaluated with the lake eutrophication evaluation method, measurement of fussy and nitrogen nutritive salt and measurement of alga biomass are not required, and rapid evaluation and type classification of lake eutrophication can be achieved in the mode that the absorption coefficients of the water body at 440 nm only need to be measured; operating is easy to carry out, the cost is low, and the method is easy to popularize, and the advantages of the lake eutrophication evaluation method are quite obvious; in addition, as the water-body absorption coefficients can be measured in an in-situ mode, the precondition can be provided for establishing a measurement method based on the in-situ lake eutrophication state index.
Owner:NANJING INST OF GEOGRAPHY & LIMNOLOGY

Rice amylose content and dextrinization temperature fast synergistic determining method

The present invention relates to a rice amylose content and gelatinization temperature quickly synergistic determination method, belonging to the field of rice quality determination technology. Said method includes the following steps: selecting sample to be determined and defining check sample, using KOH solution to make gelatinization, observing cargo rice endosperm decopmosition, recording and calculating its gelatinization temperature, fully staining the sample to be determined and check sample by using I2-KI solution and making comparison so as to quickly judge the content of rice amylose.
Owner:CHINA NAT RICE RES INST

Rapid determination method of molybdenum content in iron and alloys

The invention discloses a rapid determination of molybdenum content in iron and alloys. According to the method, an iron or alloy sample is adopted. A phosphoric acid-perchloric acid mixed solution is employed to dissolve and oxidize the sample. In a sulfuric acid-phosphoric acid medium, an ascorbic acid is used as a reducing agent to prepare a sample solution, a working curve technique is employed to calculate the molybdenum content, so that molybdenum (V) cannot be reduced to lower valence molybdenum, and the result can have good repeatability. Due to the oxidation effect of a lot of perchloric acid, the unstable color development and large determination result error phenomena arising in dissolution of a tungsten and vanadium-containing sample by sulfuric acid-phosphoric acid or reduction of the sample by stannous chloride. In the sulfuric acid-phosphoric acid medium, the color development solution is stable, and reduction needs no adding of iron as the conditioning agent, thus eliminating the influence brought by iron in the solution. During sample dissolution, there is no need to add nitric acid at the time of smoking to destroy a carbide. The analysis time is only 1 / 5-1 / 4 of that of the alpha-Benzoin oxime gravimetric method. Fewer instruments and reagents are used, thus reducing the determination cost. Compared with conventional spectrophotometry, the method broadens the mass fraction measurement range.
Owner:GUIZHOU AEROSPACE PRECISION PRODS

Method for detecting protein content

The invention relates to a method for detecting protein content. The method comprises the following steps of: preparing standard protein samples and reagents (1), (2) and (3); preparing the standard protein samples with gradient; adding the reagents (1), (2) and (3) into the gradient samples and samples to be tested by a micropore method or a standard test tube method; measuring absorbance; drawing a standard curve; and calculating the concentration according to the absorbance of the samples to be tested. By the method, the protein content can be detected accurately. The method is applicable to protein solution containing a plurality of interfering substances. By the method, the inclusiveness for the interfering substances such as a surfactant, a reducing agent, a chelating agent, a nitrogen-containing compound and the like in the protein analysis and detection process can be improved. The method is a simple, quick, high-sensitivity and high-stability method for detecting the protein concentration and has a wide application prospect in the field of life science research.
Owner:NANJING NORMAL UNIVERSITY

Method for judging source of colored soluble organic matter based on fluorescence-spectrum integral specific value

The invention provides a method for judging the source of a colored soluble organic matter based on a fluorescence-spectrum integral specific value. After a three-dimensional fluorescence spectrum of the colored soluble organic matter in a lake is measured, the source of the colored soluble organic matter is distinguished by utilizing the specific value IC to IT between a peak-C spectrum integral value of humic acid and a peak-T spectrum integral value of similar tryptophan; when the IC to IT is less than 5, the source of the colored soluble organic matter is an endogenous dominant; when the IC to IT is not less than 5 but not more than 20, the source of the colored soluble organic matter is an exogenous dominant; when the IC to IT is more than 20, the source of the colored soluble organic matter is an intensive exogenous effect. By using the method provided by the invention, the addition of a chemical reagent is not needed; the carrying out of fussy complicated calculations of the measurement of a conventional chemical index, the parallel factor analysis of a three-dimensional fluorescent component, and the like, is also not needed; the measurement of the fluorescence intensity, in several common waveband ranges, of the colored soluble organic matter is only needed; thus, the quick evaluation and division of the source of the colored soluble organic matter in an inland water body can be realized; the operability is high; the method is easy to popularize and is compared with peer research results at home and abroad; meanwhile, the fluorescence spectrum is easily modified to form an on-line or in-situ monitoring fluorescent probe; therefore, the method has important and high application value.
Owner:NANJING INST OF GEOGRAPHY & LIMNOLOGY

A method and device for rapid and automatic determination of melamine content in dairy products

The invention discloses a rapid and automatic determination method and device for tripolycyanamide content in dairy products, belonging to the field of food analysis. The method comprises the following steps that: in a unit (A) under an operating procedure, a first step, a multifunctional valve is in a state I, a pump A rotates, a pump B stops, a first sample flows into a column 2 for enriching tripolycyanamide, and the eluent flows into a column 1 for regenerating tripolycyanamide; a second step, the pump A stops, the pump B rotates, the cleanout fluid flows into the column 2 for cleaning the impurities therein; a third step, the multifunctional valve is switched to a state II, eluent flows into the column 2 for eluting and regenerating tripolycyanamide; the tripolycyanamide in the column 2 is substituted by the eluent and directly enters into a sampling ring of a unit (B) in the form of a sample plug, so as to perform volume quantification, injection, re-separation and detection, and a response signal is processed by a computer; and meanwhile, a second sample enters into the column 1 for enrichment, and the process of the column 2 is repeated. The method and the device are high in automation degree and excellent in reproducibility, and can analyze about 30 samples per hour; and the unit (A) in the invention can be used with various flow injection analysis systems.
Owner:SICHUAN UNIV

Pre-treatment method for phthalate ester plasticizers in edible oil

The invention discloses a pre-treatment method for phthalate ester plasticizers in edible oil. The pre-treatment method comprises: carrying out extraction on a certain mass of edible oil by using an organic solvent; carrying out a freezing degreasing treatment on the extraction liquid, and extracting the liquid; carrying out solid phase extraction small column purification on the obtained extraction liquid; and carrying out an elution blow drying volume metering treatment on the purified purification solution. Compare with the pre-treatment method in the national standard, the pre-treatment method of the present invention has the following characteristics that: the inspection process is short, operations are convenient, extraction efficiency is high, repeatability is good, solvent consumption is less, a measurement cost is low, sensitivity is high, contents of five common phthalate esters in edible oil can be concurrently detected, and good application prospects are provided.
Owner:JIANGNAN UNIV

Direct fitting determination method of gas content of coal seam

The invention discloses a fitting measurement method for coal seam gas content. Firstly, a fitting calculation model is established, and the general expression of the coal sample gas desorption law is deduced according to the calculation. ; Then take samples from the downhole coal core, and measure the atmospheric pressure p of the downhole measurement site sequentially 1 and temperature T 1 1. Downhole natural desorption gas volume; correct the downhole natural desorption gas volume data, and after the temperature and pressure are corrected to the standard state, the gas loss per unit mass of coal core can be calculated. In the present invention, the relative error δ between the gas content obtained by the direct fitting measurement method and the gas content measured by the indirect method is less than 10%, which meets the actual requirements of the site, and the method is feasible; the method can measure a large number of measuring points, and can be better It is widely used in coal seam outburst dangerous working face and regional prediction, pre-extraction gas effect evaluation and mine coal seam gas emission prediction, etc.
Owner:SHANDONG UNIV OF SCI & TECH

Method for determining nickel content in soil by microwave digestion-flame atomic absorption spectrometry

The invention provides a method for determining a nickel content in soil by microwave digestion-flame atomic absorption spectrometry, wherein the method is characterized by: pretreating a soil sample by combining long-time immersion and stepedly heated microwave digestion, using polyoxyethylene castor oil glycerin ether as an activator, raising atomization efficiency of nickel atoms in flame, raising determination sensitivity, thereby raising sensitivity; based on linear relation of absorbance and nickel content determined by a flame atomic abserption spectrometer by a standard solution, measuring and calculating to obtain the content of nickel atoms in soil. The method provided by the invention solves problems that a method for determining trace elements by traditional atomic absorption spectrometry has disadvantages long time consumption, low sensitivity, large solvent consumption amount, and severe environment pollution caused by a solvent, and the method provided by the invention has advantages of good precision, high recovery rate, accuracy, reliability, rapidness and convenience.
Owner:SUZHOU GUOHUAN ENVIRONMENT DETECTION

Method for measuring bromide ions and iodide ions simultaneously

The invention provides a method for measuring bromide ions and iodide ions simultaneously. According to the method, the bromide ions and the iodide ions in water are simultaneously measured by a dual-wavelength spectrophotometry according to optical adsorption properties of ultraviolet regions of the bromide ions and the iodide ions; an analysis process is simple and quick; the measurement cost is low; the method disclosed by the invention can be used for simultaneous and quick measurement of trace bromide ions and trace iodide ions in systems such as seawater, river water, lake water, underground water, drinking water and bittern.
Owner:QINGHAI INST OF SALT LAKES OF CHINESE ACAD OF SCI

Observation and density measurement method for glandular hairs on Artemisia annua leaf surfaces

The invention discloses an observation and density measurement method for glandular hairs on Artemisia annua leaf surfaces, comprising the following steps: taking a eugonic Artemisia annua leaf, and feeding the leaf in a centrifuge tube or a microtiter plate; successively adding petroleum ether or normal hexane, absolute ethyl alcohol or carbinol and glacial acetic acid, evenly mixing, and incubating for 5-20min at room temperature; and taking out the leaf, placing the left on a glass slide, adding a drop of double distilled water, covering a cover slip slightly, compacting by a tablet clip, and observing on an optical microscope or calculating the number of the glandular hairs so as to calculate the density of the glandular hairs. The method is simple and convenient, has low requirement on equipment, greatly simplifies the preprocessing procedure of experimental materials, can complete the whole operation within half a hour, uses a little of reagent, is non-toxic, safe and environment-friendly, lowers the measurement cost and time substantially, and can be used for high pass analysis.
Owner:HUNAN AGRICULTURAL UNIV

Method of rapid determination of hydrogen peroxide content of solution

The invention discloses a method for rapid determination of hydrogen peroxide content of a solution. Hydrogen peroxide is excited by ferrous iron ion, a tracer agent of p-phenylenediamine of N, N-diethyl is employed, by the test of the absorbance after the reaction of the standard sample of concentration gradient, a criterion curve and a linear regression equation of the hydrogen peroxide concentration relative to the absorbance are obtained by fitting, and the relevant hydrogen peroxide content is obtained by testing of the absorbance after the same reaction of samples by rapid determination, the method is simple in operation, accurate and sensitive, green and environmentally friendly, economical and practical, and the hydrogen peroxide content in the samples is testable rapidly in the laboratory and in-situ by the use of UV-visible spectrophotometer only. The method is widely applicable in environmental protection, chemical and medical industries, and in rapid and accurate detections of residual hydrogen peroxide content on a variety of samples by the treatment of oxidation, bleach or disinfection.
Owner:HUAQIAO UNIVERSITY

Method for testing melting speed of continuous casting powder

The invention relates to a method for testing the melting speed of continuous casting powder. The method is characterized by comprising the following steps of: weighing three parts of casting powder samples by using a scale, wherein each part is 1.49-1.51 g; in the event of raising the temperature, placing a crucible in a furnace, raising the temperature of the furnace to 1350 DEG C, wherein the temperature fluctuation error is + / - 20 DEG C, and keeping the constant temperature for 20 min; taking out the crucible from a hearth, rapidly adding a part of casting powder sample to be tested into the crucible, putting the sample together with the crucible in the furnace, simultaneously, stating a stopwatch to time, and recording the time required for completely melting the sample, namely the time for putting the sample in the hearth till the last dark spot is brightened; calculating the melting speed according to the formula MR=W / tau*A; and repetitively testing according to the above steps and calculating other samples, and then solving an average value to obtain the melting speed MR of the casting powder under the 1350 DEG C condition. The invention aims to provide the method, which is capable of simulating the one-way heating and melting condition of the casting powder on a steel level in a crystallizer and true reflecting the melting speed of the casting powder.
Owner:INNER MONGOLIA BAOTOU STEEL UNION

High-specificity microRNA fluorescence detection method based on short chain nucleic acid probe and double-chain specific endonuclease

ActiveCN107267604AHigh sensitivityHigh specificity isothermal detectionMicrobiological testing/measurementTemperature controlFluorescence
The invention discloses a high-specificity microRNA fluorescence detection method based on a short chain nucleic acid probe and a double-chain specific endonuclease. According to the invention, the short chain DNA probe is used and the DSN enzyme is combined, so that probe / miRNA hybridization rate and hybridization-digestion circulating rate are increased, and meanwhile, the sensitivity of the probe for the hybridization reaction mismatch is promoted and the miRNA high-sensitivity high-specificity constant temperature detection under human body temperature is realized; the homologous similar miRNAs of single-base mismatch can be sensitively distinguished; according to the method, the efficient amplified reaction at 37 DEG C can be acquired; compared with other amplified reaction based on DSN enzyme (under the temperature at 55-60 DEG C), the efficient amplified reaction is more suitable for in situ or vivo detection of cells; the amplified reaction is thermostatic reaction and requires no accurate temperature control or temperature circulating device; and the operation is simple, the cost is low and the method is more suitable for marketing.
Owner:SUN YAT SEN UNIV

Method for treating pyrethroid pesticide residue soil environment sample before measurement

The invention discloses a method for treating a pyrethroid pesticide residue soil environment sample before measurement, and relates to a method for analyzing and pretreating organic pollutants in soil environment samples. The method comprises the following steps: grinding a soil sample; performing freeze drying on the collected soil environment sample, grinding and crushing; extracting, namely accurately weighing the soil sample, adding petroleum ether / acetone, performing ultrasonic extraction, and adding 2 percent of sodium sulfate aqueous solution into a supernatant; concentrating, namely drying an upper-layer organic phase by virtue of anhydrous sodium sulfate, evaporating and concentrating; purifying by adopting a glass chromatography silica-gel column method, and performing wet column packing; concentrating and making up to the volume, namely dissolving and making up to the volume by using chromatographic n-hexane, and measuring the content of pyrethroid by adopting a gas chromatograph or a gas chromatograph-mass spectrometer. According to the method, multiple pyrethroid pesticide compounds can be extracted from the soil environment samples and are purified; according to the method, the cost is low, reproducibility and stability are high, and methods and technical supports are provided for detection and evaluation of pyrethroid pesticides in the soil environment samples.
Owner:SHENYANG UNIV

Method for determination of calcium sulfite and calcium carbonate content of flue gas desulfurization gypsum

InactiveCN104849173ADrive mass adoptionJudging the retarding effectWeighing by removing componentPortland cementAnalytical balance
The invention discloses a method for determination of calcium sulfite and calcium carbonate content of flue gas desulfurization gypsum and belongs to the method for determination of calcium sulfite and calcium carbonate content. The method comprises respectively dissolving flue gas desulfurization gypsum subjected to full oxidation treatment and unoxidized flue gas desulfurization gypsum by a diluted hydrochloric acid solution, determining mass losses of the flue gas desulfurization gypsum and the unoxidized flue gas desulfurization gypsum by an analytical balance in dissolution, calculating calcium carbonate content of the flue gas desulfurization gypsum according to the mass loss of the flue gas desulfurization gypsum subjected to full oxidation treatment, and calculating total content of calcium sulfite and calcium carbonate of the flue gas desulfurization gypsum according to the mass loss of the unoxidized flue gas desulfurization gypsum, wherein the difference of the calcium carbonate content and the total content of calcium sulfite and calcium carbonate is the total content of calcium sulfite of the flue gas desulfurization gypsum. Calcium sulfite of the flue gas desulfurization gypsum produces large influence on portland cement setting time and strength, and through determination of calcium sulfite and calcium carbonate content, flue gas desulfurization gypsum slow setting effects on portland cement can be determined. The method promotes large scale use of flue gas desulfurization gypsum as industrial waste residue. The method has the advantages of simple determination method, accurate result and low determination cost.
Owner:CHINA UNIV OF MINING & TECH +1

Method for preparing attapulgite clay catalyst and method for measuring COD by the catalyst

The invention discloses a method for preparing an attapulgite clay catalyst and a method for measuring COD by the catalyst. The method for preparing the attapulgite clay catalyst comprises the following steps: (1) attapulgite purification, namely adding the primary attapulgite into water according to a mass ratio of 1:10-15, stirring the mixture for 24 to 48 hours to form attapulgite slurry, keeping the attapulgite slurry standing naturally, taking suspension on the upper layer, and filtering, drying and grinding the suspension to obtain purified attapulgite; and (2) acidification treatment, namely adding the purified attapulgite into mixed acid solution according to a mass ratio of 1:10-15, slightly boiling the mixture for 70 to 90 minutes by continually stirring, filtering the boiled mixture, washing the filter cake to be neutral, and drying and grinding the filter cake to obtain acidified attapulgite, namely the attapulgite catalyst, wherein the mixed acid solution of which volume concentration is 5 percent is formed by mixing sulphuric acid and phosphoric acid. The steps in the COD measuring method are the same as GB11914-1989; when domestic sewage is measured, 20 milliliters of concentrated sulphuric acid and 0.3 gram of attapulgite are added into the domestic sewage and reflowed for 30 minutes; and when industrial sewage is measured, 30 milliliters of concentrated sulphuric acid and 0.3 gram of acidified attapulgite are added into the industrial sewage and reflowed for 30 minutes. By adopting the primary attapulgite to prepare the catalyst, the development and utilization values of the primary attapulgite are promoted; and by adopting the acidified attapulgite to measure COD, the measuring cost is lowered, the measuring time is shortened, and the secondary pollution is reduced.
Owner:HUAIYIN TEACHERS COLLEGE

Method of determining silver content in rock minerals

The invention discloses a method of determining silver content in rock minerals. The method comprises the following steps: preparing a silver standard storage solution; preparing a silver standard solution; determining absorbance of the silver standard storage solution by using an atomic absorption spectrometer and a silver hollow cathode lamp and drawing a working curve; taking a sample; adding nitric acid, hydrochloric acid, hydrogen peroxide, an ammonium fluoride solution and a sodium chlorate solution, carrying out microwave digestion, heating and drying an obtained mixed solution with steam, adding hydrochloric acid and nitric acid, dissolving solids, transferring an obtained solution to a volumetric flask, diluting the solution with distilled water until the liquid level of the solution reaches a scale mark, shaking up the solution, determining absorbance of silver in the obtained sample solution by using the atomic absorption spectrometer and the silver hollow cathode lamp, carrying out blank tests on associated samples, and calculating the concentration of silver in the sample according to a formula. The invention has the advantages of convenience, fastness, a small usage amount of reagents, low cost for determination and no discharge of a great amount of acid gas, is favorable for protecting environment and health of operational personnel, avoids loss of the sample inthe sample solution, enables precision of analysis to be enhanced, labor intensity to be reduced, time to be saved and work efficiency to be improved, and has a wide application scope.
Owner:山东正元地质资源勘查有限责任公司

Visible spectrophotometric method for concentration of hydrogen peroxide in water

The invention discloses a visible spectrophotometric method for the concentration of hydrogen peroxide in water. The visible spectrophotometric method has the benefits that the hydrogen peroxide is catalyzed by catalase, 2'-hydrazine-bis-3-ethylbenzothiazoline-6-sulfonic acid is taken as an indicator, and through test on the absorbance after the reaction of a standard sample of a concentration gradient, a standard curve for the concentration of the hydrogen peroxide relative to the absorbance as well as a linear regression equation are obtained by fitting; then the corresponding hydrogen peroxide concentration is obtained through determining the absorbance after the same reaction of a to-be-tested sample. The visible spectrophotometric method is a method capable of quickly detecting the content of the hydrogen peroxide in the sample in a laboratory and on site only by using a visible spectrophotometer, which is simple and convenient to operate, accurate and sensitive, green and environment-friendly, and economic and practical. The visible spectrophotometric method disclosed by the invention can be widely applied to environmental protection, cosmetics and medical industries and can quickly and accurately detect the residual content of the hydrogen peroxide in various oxidized, bleached or sterilized samples, rainwater and a specific Fenton system.
Owner:HUAQIAO UNIVERSITY

Method and kit for determination of human POT1 gene rs1034794 site polymorphism

The invention discloses a method and a kit for determination of human POT1 gene rs1034794 site polymorphism. The method is as follows: providing to-be-tested human genomic DNA; providing upstream and downstream primers for amplification of sequences nearby human POT1 gene rs1034794 site, wherein the upstream primer has a mismatched base A; taking the to-be-tested human genomic DNA as a template, using the upstream and downstream primers for PCR amplification reaction to obtain an amplification product containing AGCW fragment, wherein W is to be confirmed base A or T on the human POT1 gene rs1034794 site; providing a restriction endonuclease; using the restriction endonuclease for enzyme digestion of the amplification product to obtain a corresponding enzyme digestion product; and according to the resulting enzyme digestion product, determining whether the to be confirmed base W on the human POT1 gene rs1034794 site is A or T. The restriction endonuclease is restriction endonuclease which only be capable of cutting one of AGCA fragment or AGCT fragment. The determination method is fast and reliable, and can greatly reduce the determination cost.
Owner:ZHENGZHOU UNIV

Fluorescent capillary reaction apparatus and fluorescent capillary analytical method

This invention discloses one fluorescent capillary reaction device and it analysis method, wherein the device comprises fixing socket, capillary insert hole, laser emission window, fluorescent window and capillary. The method comprises the following steps: executing FCA method; putting the socket into fluorescent light path; inserting capillary into liquid to be tested into socket top; the laser passing socket emission window on capillary to generate fluorescent light from fixed socket emission window through single color device at tester.
Owner:SICHUAN UNIV

Determination method of silicon content in silicomanganese

The invention belongs to the technical field of element determination, and particularly relates to a determination method of silicon content in silicomanganese. The determination method of silicon content in silicomanganese comprises the following steps: firstly, after fusing a sample with sodium hydroxide, leaching with hot water; adding sulfuric acid for acidification; after carrying out constant volume sampling, adjusting the pH value; adding mixed acid of sulfuric acid and nitric acid and ammonium molybdate, silicon in silicomanganese and ammonium molybdate generate silicate molybdate acid; finally, adding ferrous sulfate for restoring silicate molybdate acid into silicomolybdenum blue, and determining the absorbance at the wave length of 680 nm with a spectrophotometer. The determination method can easily, conveniently, efficiently and accurately analyze silicon content in silicomanganese, so as to meet the fast-paced production demand and reduce the determination cost.
Owner:SHANDONG XIWANG SPECIAL STEEL

Device and method for simultaneously measuring multi coal seams gas pressure based on downward penetrating drilling

The invention discloses a device and method for simultaneously measuring multi coal seams gas pressure based on downward penetrating drilling. The pressure measuring device comprises a casing, whereinthe casing is arranged in a drill hole and is communicated with a plurality of coal seams and rock strata, a flange plate, wherein the flange plate corresponding to the position of a top bottom plateof the coal seam is arranged on the casing, an isolation bag, wherein the isolation bag is fixed on the pipeline adjacent to the flange plate and the isolation bag is used for isolating an air chamber and a cement mortar filling chamber so as to prevent the cement mortar from flowing into the air chamber. Each pipe in the casing is divided into two purposes of grouting and pressure measurement, an isolated space and an orifice between adjacent isolation bags are respectively connected with a grouting pipe and a pressure measurement pipe in the casing, an isolated space connected with a rock stratum is the cement mortar filling chamber, the cement mortar filling chamber is communicated with the orifice through the grouting pipe and is used for grouting, the orifice end of the grouting pipeis connected with a grouting pump and grouting is carried out to the cement mortar filling chamber; the isolated space connected with the coal seam is an air chamber which is communicated with the orifice through a pressure measuring pipe, and the orifice end of the pressure measuring pipe is connected with a pressure gauge and used for measuring pressure. The pressure measuring method carries out layered pressure measurement based on the device. The device and the method for simultaneously measuring multi coal seams gas pressure based on downward penetrating drilling has the advantages of good pressure measuring effect, low construction cost, simple and easily-obtained materials, and can effectively solve the problems of large construction amount and high measurement cost for independently measuring the gas pressure of the coal seam group and the like.
Owner:CHINA UNIV OF MINING & TECH (BEIJING)
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