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Visible spectrophotometric method for concentration of hydrogen peroxide in water

A technology of hydrogen peroxide concentration and hydrogen peroxide, which is applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of toxic test reagents, large errors in test results, complex and cumbersome operations, etc., and achieve cheap and easy-to-obtain medicines and reliable results , the effect of cheap equipment

Inactive Publication Date: 2017-12-08
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention proposes a visible spectrophotometric measurement of hydrogen peroxide concentration in water in order to solve the problems of complex and cumbersome operation, large measurement error, long measurement time, toxic reaction reagent and high measurement cost in the existing hydrogen peroxide determination method. method

Method used

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  • Visible spectrophotometric method for concentration of hydrogen peroxide in water

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Embodiment 1

[0022] The visible spectrophotometry method of hydrogen peroxide concentration in a kind of water of the present embodiment, it is to carry out according to the following steps:

[0023] Step 1: Make a standard curve:

[0024] 1. Add 0.05mL of catalase mother liquor and 0.1mL of 2'-hydrazine-bis-3-ethylbenzothiazoline-6-sulfonic acid sulfate mother liquor to 2.3mL of phosphate buffer solution to obtain Mix solution A, measure the absorbance value of mixed solution A at 415nm, and zero;

[0025] 2. Add 0.05mL hydrogen peroxide standard solution with different concentration gradients to mixed solution A to form mixed solution B, with a total reaction volume of 2.5mL;

[0026] 3. After reacting at room temperature for 30 seconds, measure the absorbance value of the mixed solution B at 415 nm after the reaction, denoted as b;

[0027] 4. With b as the abscissa and the final concentration of hydrogen peroxide in the mixed solution B as the ordinate, establish a standard curve and...

Embodiment 2

[0041] The rapid assay method of hydrogen peroxide concentration in the initial rainwater of the present embodiment, it is to carry out in the following steps:

[0042] Step 1: Make a standard curve:

[0043] 1. Add 0.05mL catalase mother liquor and 0.1mL 2'-hydrazine-bis-3-ethylbenzothiazoline-6-sulfonic acid sulfate mother liquor to 1.65mL phosphate buffer solution to obtain mixed solution A , measure the absorbance value of the mixed solution A at 732nm, and zero;

[0044] 2. Add 0.05mL of hydrogen peroxide standard solutions with different concentration gradients to mixed solution A to form mixed solution B, with a total reaction volume of 3.2mL;

[0045] 3. After reacting at room temperature for 15 seconds, measure the absorbance value of the mixed solution B at 732nm after color development, and record it as a;

[0046] 4. Take a as the abscissa and the final concentration of hydrogen peroxide in the mixed solution B as the ordinate to establish a standard curve and it...

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Abstract

The invention discloses a visible spectrophotometric method for the concentration of hydrogen peroxide in water. The visible spectrophotometric method has the benefits that the hydrogen peroxide is catalyzed by catalase, 2'-hydrazine-bis-3-ethylbenzothiazoline-6-sulfonic acid is taken as an indicator, and through test on the absorbance after the reaction of a standard sample of a concentration gradient, a standard curve for the concentration of the hydrogen peroxide relative to the absorbance as well as a linear regression equation are obtained by fitting; then the corresponding hydrogen peroxide concentration is obtained through determining the absorbance after the same reaction of a to-be-tested sample. The visible spectrophotometric method is a method capable of quickly detecting the content of the hydrogen peroxide in the sample in a laboratory and on site only by using a visible spectrophotometer, which is simple and convenient to operate, accurate and sensitive, green and environment-friendly, and economic and practical. The visible spectrophotometric method disclosed by the invention can be widely applied to environmental protection, cosmetics and medical industries and can quickly and accurately detect the residual content of the hydrogen peroxide in various oxidized, bleached or sterilized samples, rainwater and a specific Fenton system.

Description

technical field [0001] The invention relates to a rapid determination method of hydrogen peroxide in water, in particular to a method using catalase as a catalyst and 2'-hydrazine-bis-3-ethylbenzothiazoline-6-sulfonic acid as an indicator Visible spectrophotometric method for rapid determination of hydrogen peroxide concentration in water. Background technique [0002] Due to its strong oxidizing ability and environmental protection features, hydrogen peroxide is widely used as oxidant, disinfectant, sterilant and bleaching agent in environmental protection, daily chemical, food, medical and printing and dyeing industries. In addition, hydrogen peroxide can also be activated by transition metal ions, metal oxides, microwave radiation, and ultraviolet radiation to generate hydroxyl radicals with stronger oxidizing ability (E 0 =1.8-2.7V), is widely used in the field of environmental protection, especially in the field of water treatment to remove refractory organic pollutant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 邹景蔡婳婳肖钧洋刘鑫庞子君苑宝玲
Owner HUAQIAO UNIVERSITY
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