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Quantitative detecting method for the polyphenol content

A quantitative detection method and technology of tea polyphenols, which are applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of catechin monomers. Difficult to purchase, expensive HPLC instruments, and limited range of use, etc., to achieve the effect of reduced measurement time and cost, low environmental conditions, and low measurement environment and conditions.

Inactive Publication Date: 2006-08-02
CHINA AGRI UNIV
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Problems solved by technology

However, HPLC instruments are expensive and have high requirements for operators. At the same time, it is difficult to purchase high-purity catechin monomers, so the scope of use is not wide.

Method used

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Embodiment Construction

[0020] The invention provides a quantitative detection method for the content of tea polyphenols. The present invention organically combines the reagent of ferrous tartrate with the principle of colorimetry, utilizes tea polyphenols and ferrous tartrate to form a blue-purple complex, and makes samples with different contents of tea polyphenols. The ferrous tartrate reagent forms blue-purple complexes with different shades. Using the principle that its chromaticity value is highly linearly correlated with the content of tea polyphenols, it is possible to obtain an improved method for calculating the content of tea polyphenols by measuring the absorbance value with a spectrophotometer. The same detection accuracy as the ferrous tartrate method.

[0021] The specific detection steps of the method are as follows:

[0022] Step 1, prepare the used ferrous tartrate reagent of chromogenic reaction;

[0023] Step 2: Prepare standard sample solution, weigh 0.5, 1.0, 1.5, and 2.0 gram...

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Abstract

The present invention discloses a quantitative detection method of tea-polyphenol content. Said method includes the following steps: preparing standard sample liquor and color-developing liquor of sample to be detected, then placing proper quantity of color-developing liquor into colorimetric cell on the image collector, collecting original image information; utilizing tea-polyphenol content determination software system based on computer chromaticity identification to read collected original image, comparing chromaticity value of standard sample liquor with known tea-polyphenol content and drawing standard curve, obtaining chromaticity information of sample liquor to be detected so as to obtain the tea-polyphenol content of detected sample liquor.

Description

technical field [0001] The invention belongs to the technical scope of quantitative detection of chemical components, in particular a method for quantitative detection of tea polyphenol content based on the combination of image analysis and ferrous tartrate colorimetry. Background technique [0002] Currently available methods for the determination of tea polyphenols mainly include ferrous tartrate colorimetry, potassium permanganate titration and high performance liquid chromatography (HPLC). [0003] The potassium permanganate titration method has been used for a long time, and it is a simple and easy method improved by the A.O.A.C method. But this method shows all the substances that can be oxidized, especially for those crude tea polyphenols with low content of tea polyphenols and high content of other impurity components (such as Vc, etc.), the result is often high, and the titration end point is different. Sensitive and difficult to grasp, different people often have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 侯彩云冯婧李霄王小伟耿然
Owner CHINA AGRI UNIV
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