The invention discloses a magnetic
enzyme nanogel biocatalytic particle and a preparation method thereof. The magnetic
enzyme nanogel biocatalytic particle is of a core-shell structure, the core of the magnetic
enzyme nanogel biocatalytic particle is a magnetic
nanoparticle with enzyme molecules adsorbed on the surface, and the enzyme molecules react with the magnetic
nanoparticle through electrostatic force; the shell is a crosslinked high-
polymer material layer; and the core is connected with the shell through a
chemical bond. The preparation method is as follows: an amino group, a carbon-carbon
double bond, a carboxyl group and other groups are first introduced into the surface of the magnetic
nanoparticle by way of
chemical modification, enzyme-particle nanoscale
composite material is then obtained by regulating the pH of solution, and finally, with a vinyl
monomer as material, the magnetic enzyme nanogel biocatalytic particle is obtained by way of free
radical polymerization. The method is characterized in that: the method is simple and mild, purification is convenient, and
industrial scale-up is easy to implement. The magnetic enzyme nanogel biocatalytic particle has the characteristics of high biocatalytic activity and high
thermal stability, is convenient to recycle, and has a broad application prospect in
organic synthesis, the
food industry, the detergent industry, the energy industry,
biomedicine, sensors and other fields.