The invention discloses an immobilization method and application of
pichia pastoris. Fibrous materials after being pretreated by
plant glue are used as an immobilization carrier, the
pichia pastoris is activated, then immobilization
fermentation culture mediums are inoculated with the activated
pichia pastoris, culture is performed, strains are adsorbed on the carrier in the culture process. In addition, the fibrous materials after being pretreated by the
plant glue can be soaked in a methyl acryloxyethyl trimethyl
ammonium chloride aqueous solution for secondary pretreatment, then washing anddrying are performed, so that the
pichia pastoris is obtained. The immobilization method has the beneficial effects that through treatment with methyl acryloxyethyl trimethyl
ammonium chloride, the surface of the carrier is provided with positive charges; the surfaces of materials are accessed with the positive charges of appropriate density, so that adsorption of proteins adhered on
cell surfaces can be effectively strengthened,
cell adhesion can be promoted, and besides, negative influence cannot be produced on the activity of cells; the
enzyme activity of products is increased, the enzymeactivity of
phytase produced through a fermenter is increased from 12326.85U / mL to 15221.85U / mL; and the whole
fermentation cycle can be shortened.