The invention relates to the technical field of functional polyester film, particularly relates to an anti-fog polyester film and a preparation method thereof, for solving the problems of thick thickness, bad thermostability, difficulty in applying in high-temperature high-humidity environment in existing anti-fog films. The anti-fog polyester film disclosed herein comprises a polyester base material, wherein the two sides of the base material is provided with anti-fog function layers, the polyester base material is a pure polyester layer, the material of the anti-fog function layers comprises 100 parts of polyester slices, 0.5-2.0 parts of anti-fogging agent, and 0.1-0.5 parts of synergist. The film disclosed herein has good transparency, good machining property, thin thickness, good thermostability, and good anti-fog performance, and can be applied in high-temperature high-humidity environment. The preparation method of the film is simple and easy to operate.