The invention provides a method for extracting fructus
cannabis oligopeptide from a fructus
cannabis cake. The method comprises the following steps: collecting the fructus
cannabis cake with the
oil content of lower than 1%, adding water, regulating the pH value to 9.0, adding
sodium metabisulfite, carrying out
solid-liquid separation, and collecting the
liquid phase; adding a HCl solution into the
liquid phase, regulating the pH value to 3.8-4.3, standing, carrying out secondary
solid-liquid separation, and collecting the precipitate; adding water, heating to 40-55 DEG C, regulating the pH value to 7.0-9.0, adding a compound
protease, carrying out enzymolysis for 3-6 hours, carrying out
centrifugal filtration, and collecting the clear solution which is the fructus cannabis
oligopeptide solution; and carrying out
electrodialysis, concentration and freeze-
drying to obtain the fructus cannabis
oligopeptide powder. The fructus cannabis cake subjected to press deoiling is used as the
raw material, the
isoelectric point principle is combined to precipitate the
protein, and the compound
protease enzymolysis and freeze-
drying are performed to obtain the fructus cannabis oligopeptide. The whole process utilizes the pure physical technique, and no chemical component is added; and thus, the method has the advantages of low cost and no environment
pollution.