Microorganism cultured birds padding additive and method for making same
A technology of microbial breeding and treatment agent, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as inability to effectively decompose feces and other problems, and achieve a situation that is conducive to growth and development, improves the breeding environment, and reduces odor. Effect
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Embodiment 1
[0046] The first step. Breeding and production of strains:
[0047] (1). Using Streptomyces flavinus, Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus casei provided by the Culture Preservation Center of the Ministry of Agriculture, Streptomyces luteus is cultivated by potato medium (PDA), Bacillus licheniformis, Lactobacillus plantarum 1. Lactobacillus casei was subcultured on nutrient agar medium, and it was observed that the growth rate of the bacteria was fast. The colony diameter of Lactobacillus casei and Lactobacillus plantarum was greater than 3ml, Streptomyces luteus was greater than 2ml, and Bacillus subtilis was greater than 5ml at 0°C-4 ℃ temperature to preserve;
[0048] (2). Production performance test: inoculate Streptomyces luteus on potato medium (PDA) for cultivation, and inoculate three kinds of strains of Bacillus licheniformis, Lactobacillus plantarum and Lactobacillus casei into liquid nutrient agar medium respectively, and cultivate The te...
Embodiment 2
[0067] The first step. Breeding and production of strains:
[0068] With embodiment 1;
[0069] Step 2: Liquid high-speed culture of compound strains
[0070] (1). Take each strain according to the weight percentage below the right; Streptomyces luteus 34%, Bacillus licheniformis 23%, Lactobacillus plantarum 26%, Lactobacillus casei 17%;
[0071] (2). Liquid culture of compound strains: Mix the Lactobacillus plantarum and Saccharomyces cerevisiae bacteria weighed according to the ratio of step 1, and then carry out compound culture together: inoculate the mixed bacterial lawn at 126°C in a ratio of 1:25 , 45min high-temperature sterilized liquid culture medium, stirred in a fermenter for 35min, and its rotating speed was 220r / min, after stirring evenly, put it into a sterilized plastic bucket and let it stand for 7 days, measure the Ph value every day during the standstill process and Release the produced gas; take samples 3 times during the fermentation process, detect the ...
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