The invention discloses a preparation method of rice
bran nutrient. The rice
bran nutrient extracted and separated by using the preparation method contains a plurality of
nutrition constituents such as rice
bran protein, rice bran
polysaccharide, rice bran
fiber,
phytic acid, minerals and the like. The preparation method is simple and helpful for the industrial large-scale production. The preparation method disclosed by the invention comprises the following steps of: (1) cleaning,
drying and crushing 1000 parts of rice bran, screening by using a 80-mesh
sieve, carrying out muggy and high-temperature vapour treatment at 85 DEG C for 5-10 min, gradually heating up to 105 DEG C in 10-15 min, and carrying out heat preservation treatment for 10-15 min; (2) adding the materials obtained in the step (1) into
petroleum ether with the weight of 2-3 times, stirring at 55-58 DEG C for 0.5-0.8 h, filtering and
drying filter residue at 105 DEG C for 0.5-1.5 h; (3) mixing the materials obtained in the step (2) by using a
hydrochloric acid water solution with the pH value of 5.0-5.5 in the material-liquid weight ratio of 1:(8-10), adding 15-25 parts of combined
enzyme A, keeping at 38-45 DEG C, stirring and reacting for 1-2 h, carrying out centrifugalization at 3500-4000 r / min, and collecting supernate; (4) adding 15-20 parts of combined
enzyme B into the supernate obtained in the step (3), keeping at 55-60 DEG C, stirring and reacting for 1-2 h, carrying out centrifugalization at 3500-4000 r / min, and collecting the supernate; and (5) carrying out vacuum concentration at 50-55 DEG C on the supernate obtained in the step (4), and carrying out
freeze drying at subzero 20-25 DEG C to obtain the rice bran
nutrient.