The invention discloses a construction method for a movable mold frame for a lock chamber wall of a ship lock. The specific construction process flow is as follows: carrying out construction preparation, assembling a movable mold frame, assembling a large template, positioning and mounting the large template, concrete pouring a single-section lock chamber wall, a movable mold frame driving the large template to walk to the next section of the lock chamber wall, disassembling the large mold plate, and disassembling the movable mold frame. According to the construction method of the movable mold frame for the lock chamber wall of the ship lock, in cast-in-place gravity type concrete lock chamber wall pouring area, compared with traditional construction adopting all-round scaffolds and simple mold frames, the labor intensity is greatly reduced, the labor amount is reduced, and the construction progress is accelerated; and the movable mold frame operation is simple, convenient and rapid, the safety and stability performance of the movable mold frame are guaranteed, the construction efficiency is high, the construction task of the lock chamber wall is completed by quality guarantee and maintenance in the node construction period, and meanwhile, the whole large template is used, and therefore the appearance quality of the lock chamber wall is effectively improved.