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Industrial high-density mixed fermentation culture medium of bifidobacterium and lactobacillus, fermentation culture method and fungus powder embedding method

A bifidobacterium and lactobacillus technology, applied in the direction of bifidobacteria, microbial-based methods, biochemical equipment and methods, etc., can solve complex industrial production methods, high cost of culture medium, low in vitro survival rate of bacterial powder, etc. problems, to achieve the effect of easy purchase, simple ingredients, and improved survivability

Active Publication Date: 2018-07-13
山东凤凰生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of high medium cost, complex industrial production method and low in vitro survival rate of bacterial powder in the current co-cultivation of probiotics, and at the same time increase the viable count of lactic acid bacteria in industrial production, the invention provides a direct-injection dual The high-density mixed fermentation medium, fermentation culture method and large-scale production fermentation method of Mycobacterium and Lactobacillus, through screening and optimizing different culture media and culture methods, and taking some comprehensive measures such as simple and practical metabolic regulation methods, make the culture medium The density of lactic acid bacteria reaches 10 10 cfu / ml

Method used

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  • Industrial high-density mixed fermentation culture medium of bifidobacterium and lactobacillus, fermentation culture method and fungus powder embedding method
  • Industrial high-density mixed fermentation culture medium of bifidobacterium and lactobacillus, fermentation culture method and fungus powder embedding method
  • Industrial high-density mixed fermentation culture medium of bifidobacterium and lactobacillus, fermentation culture method and fungus powder embedding method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, mixed strain high-density fermentation medium screening:

[0063] 1. The influence of different carbon and nitrogen sources on the number of live bacteria fermented by mixed strains:

[0064] According to the analysis of the lactobacillus and bifidobacterium formulations involved in the current workshop and literature, the main components of the medium are glucose, lactose, fructooligosaccharides, peptone, soybean peptone, yeast powder, and beef extract, and an orthogonal test with 7 factors and 2 levels is carried out , and the results of the percentage content of the 7 factors of serial numbers 1-8 are orthogonally obtained, and the serial numbers 1-8 are weighed according to the percentage content of the 7 factors in turn, and then the basic medium components are added. The basic medium components are: dipotassium hydrogen phosphate 1.35%, sulfuric acid Magnesium 0.02%, Tween 0.1%, L-cysteine ​​hydrochloride 0.05%, adjust pH6.8-7.0, prepare 8 kinds of c...

Embodiment 2

[0081] Embodiment 2, a kind of high-density mixed culture and large-scale production fermentation method of direct injection type bifidobacterium and lactobacillus, described method comprises the following steps:

[0082] Step 1, strain activation culture:

[0083] The MRS and TPY slant medium were adjusted to pH 6.5 with 6mol / L sodium hydroxide, and sterilized at 121°C for 15 minutes by moist heat for use; The ampoules of bifidobacteria were placed at room temperature for 2 hours, and then the surface of the ampoule was wiped with 75% alcohol cotton balls in the aseptic operating table, and the ampoule was opened, and 2-3 rings were picked with sterilized inoculation loops, and evenly scratched. Line, Lactobacillus plantarum was inoculated on MRS slant medium, Bifidobacterium longum was inoculated on TPY slant medium, and cultured at 30°C under anaerobic conditions for 48 hours, the culture was terminated, and the morphology of bacteria was checked under the microscope. Acti...

Embodiment 3

[0102] Embodiment 3, a kind of high-density mixed culture and large-scale production fermentation method of direct injection type bifidobacterium and lactobacillus, described method comprises the following steps:

[0103] Step 1, strain activation culture:

[0104]Adjust the pH value of the MRS and TPY slant medium to 7.0, and sterilize at 121°C for 30 minutes before use; The ampoules were placed at room temperature for 3 hours, and then the surface of the ampoule was wiped with 75% alcohol cotton balls in the aseptic operation table, the ampoule was opened, and 2-3 loops were picked with a sterilized inoculation loop, and the lines were drawn evenly. Lactobacillus lysus was inoculated on the MRS slant medium, and Bifidobacterium lactis was inoculated on the TPY slant medium, cultured under anaerobic conditions at 37°C for 24 hours, the culture was terminated, the morphology of the bacteria was checked under the microscope, and continuous activation after no pollution 2 Secon...

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Abstract

The invention provides an industrial high-density mixed fermentation culture medium of bifidobacterium and lactobacillus, a fermentation culture method and a fungus powder embedding method. The fermentation culture medium is prepared from the following components: 0.3 to 1.5 percent of corn slurry dry powder, 0 to 1.8 percent of soya peptone, 0 to 1 percent of fructooligosaccharide, 0.5 to 1 percent of glucose, 0.4 to 1.35 percent of dipotassium phosphate, 0.4 percent of potassium dihydrogen phosphate, 0.015 to 0.02 percent of magnesium sulfate, and 0.05 percent of L-cysteine hydrochloride; orthe fermentation culture medium is prepared from the following components: 0.3 to 1.5 percent of the corn slurry dry powder, 0 to 1 percent of beef extract, 0 to 1 percent of the fructooligosaccharide, 0.5 to 1 percent of the glucose, 0.4 to 1.35 percent of the dipotassium phosphate, 0.4 percent of the potassium dihydrogen phosphate, 0.015 to 0.02 percent of the magnesium sulfate and 0.05 percentof the L-cysteine hydrochloride; the fermentation method comprises strain activation and culture, first-grade strain culture, second-grade seed solution preparation, 200L small tank culture in a workshop and 1T large tank culture in the workshop. Comparing the relatively traditional TPY culture medium, the viable count improves 10 times or more and the cost is reduced by 60 percent, meanwhile, according to the formula, raw materials are easy to obtain and the components are simple, so that the fermentation culture medium is suitable for industrial production requirements. The fungus powder embedding method is adopted so that the human body and external environment tolerance capability of strains is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of probiotic product preparation, and relates to an industrialized high-density mixed fermentation medium of bifidobacteria and lactobacilli, a fermentation culture method and a bacteria powder embedding method. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. The general term for active microorganisms. There are a huge number of microorganisms inhabiting the human gut, and their composition and activities affect the host's immunity, mucosal development, and metabolism of nutrients and drugs. As one of the main types of probiotics, lactic acid bacteria play an important role in maintaining the balance of intestinal flora, reducing cholesterol, reducing endot...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23L33/135A23P10/30C12R1/225C12R1/01
CPCA23L33/135A23P10/30C12N1/04C12N1/20A23V2002/00A23V2400/11A23V2400/51A23V2200/3204A23V2300/10A23V2300/20
Inventor 单宝龙王静任宝涛李晓颖张颜廷刘虹
Owner 山东凤凰生物科技股份有限公司
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