The invention relates to a preparation method of a novel
bacteriostatic agent by using dihydromyricetin collaborative
organic acid as a
raw material. The preparation method of natural
bacteriostatic agent, which adopts dihydromyricetin (purity being not lower than 95%) collaborative
lactic acid and
citric acid as the main materials, comprises the following steps of: using 68%
alcohol to dissolve dihydromyricetin, so that the
mass concentration of the dihydromyricetin is regulated to 5g / L; adding the
lactic acid and the
citric acid in sequence under 25 DEG C, so that the contents of the
lactic acid and the
citric acid respectively achieve 0.08% and 0.1% of the total amount; using a 200rpm shaking table for uniformly mixing for 10 minutes; using
filter paper to filer; and getting the filtrate to obtain the efficient natural
bacteriostatic agent. The bacteriostatic agent has better bacteriostatic action to
bacteria and mycete, and a bacteriostatic effect equivalent to that of
potassium sorbate according to experimental comparison, and a remarkable
preservative and fresh-keeping effect in aquatic products. The bacteriostatic agent can be used for causing
bacteria cell membranes to break, so that
apoptosis is caused by the reinforced permeability of the
cell membranes. The dihydromyricetin has a plurality of healthcare functions and stable components; the collaborative
organic acid is considered as GRAS (Generally Regarded as Safe), and can be extensively used for preservation and freshness-keeping of the food.