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Danhong injection UPLC fingerprint detection method

The technology of Danhong injection and fingerprint spectrum is applied in the research field of quality control of traditional Chinese medicine, which can solve the problems of limited index components and long time consumption, and achieve the effects of reliable method, simple operation and high precision.

Active Publication Date: 2015-11-04
SHANDONG DANHONG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the above method has the defects of long time-consuming and limited detection index components.

Method used

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  • Danhong injection UPLC fingerprint detection method
  • Danhong injection UPLC fingerprint detection method
  • Danhong injection UPLC fingerprint detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Establishment of a method for fingerprint determination of Danhong injection

[0036] 1.1 Instrument

[0037] Ultra-high liquid chromatograph, (Waters ACQUITY UPLC system, American Waters company), pure / ultra-pure water instrument (Milli-Q, American Millipore company), vortex oscillator (Votex-5, manufactured by Qilin Bell Instrument, Haimen City) Co., Ltd.), low-temperature high-speed centrifuge (J25, American Thermo Forma company), one hundred thousandth balance (METTLER TOLEDO AX205, Swiss METTLER company), ultrasonic cleaning machine (SCIENTZ SB 25-12DTN, Ningbo Xinzhi Biological Technology Co., Ltd. the company).

[0038] 1.2 Test drug

[0039] Standard products: Adenosine (batch number: 110879-200202), uridine (batch number: 110887-200202), phenylalanine (batch number: 140676-200405), 5-hydroxymethylfurfural (5-HMF, batch number: 111626- 200906), protocatechuic acid (batch number: 110809-200604), protocatechuic aldehyde (batch number: 110810-200506), caffeic ...

Embodiment 2

[0085] Example 2: Establishment of fingerprints of 30 batches of Danhong injection

[0086] 2.1 Preparation of test solution

[0087] Precisely measure 2 mL of 30 different batches of Danhong injection, put them in a 10 mL brown measuring flask, add 10% methanol to the mark, and shake well.

[0088] 2.2 Chromatographic conditions:

[0089] Column: ACQUITY HSS T3 (2.1×100mm, 1.8μm); the mobile phase uses 0.1% formic acid water as mobile phase A and acetonitrile as mobile phase B. The volume ratio is that mobile phase A accounts for 97% to 3%, and mobile phase B accounts for 3 ~97%, gradient elution; the specific elution volume concentration ratio is:

[0090] At 0 min, the volume ratio of mobile phase A is 97%, and the volume ratio of mobile phase B is 3%;

[0091] At 7 min to 13 min, the volume ratio of mobile phase A is 81%, and the volume ratio of mobile phase B is 19%;

[0092] At 18 minutes, the volume ratio of mobile phase A is 75%, and the volume ratio of mobile phase B is 25%;

[...

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Abstract

The present invention relates to a Danhong injection UPLC fingerprint detection method. The chromatographic conditions are as below: a chromatographic column of HSS T3column, mobile phases including a mobile phase A of 0.1% formic acid water and a mobile phase B of acetonitrile in the volume ratio of 97%-3%: 3-97%; gradient elution; detection wavelength of 286nm, 254nm; column temperature at 30-50 DEG C, flow rate of 0.3-0.5mL.min-1, and the injection volume of 2 muL. The Danhong injection standard fingerprint generated by the method has 24 characteristic fingerprint peaks. The detection method provided by the invention has the characteristics of high precision, stabilization, good reappearance and simple method, and can rapidly and accurately discriminate the authenticity, advantage and disadvantage of the product.

Description

Technical field [0001] The invention relates to the research field of quality control of traditional Chinese medicine, in particular to a method for detecting UPLC fingerprint spectrum of Danhong injection. Background technique [0002] Fingerprint is a quality control method to effectively control the quality of Chinese medicinal materials or Chinese patent medicines proposed for the diversity and complexity of Chinese medicine chemical components. As the overall characterization of chemical substances in traditional Chinese medicine compound preparations, the fingerprint of traditional Chinese medicine can express the integrity of traditional Chinese medicine compound by integrating the local characteristics of quantitative determination of multiple index components. It has the characteristics of integrity, comprehensiveness, hierarchy, relevance and dynamics, and is especially suitable for To meet the needs of research on complex systems of traditional Chinese medicine. In vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 王跃飞赵步长朱彦赵涛胡利民王一民贾力夫焦玉娇赵菁江振作杨静千珂
Owner SHANDONG DANHONG PHARMA
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