Apocynum extract and extracting method thereof
A kind of technology of Apocynum extract and extraction method, which is applied in the direction of drug combination, medical formula, metabolic diseases, etc., can solve the problems of inability to obtain exact characterization, high ointment rate, etc., and achieve good drug development prospects, convenient administration, and high utilization value Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0058] 1. Preparation of high-purity extract of Apocynum apocynum
[0059] Get 300g of Apocynum basil leaves (baked at 200°C for 1 hour), at a temperature of 85°C-100°C, reflux extract with 3000mL of 40% ethanol water for 3 times (3000mL×3), each time for 0.5hr, combine the extracts, Stand overnight at room temperature, filter, remove precipitation, concentrate the filtrate under reduced pressure (60°C, 0.08-0.1MPa), to a total volume of 900mL, add 180mL of 1% chitosan acetic acid solution to the concentrate (equivalent to 1.8 grams of chitosan , other concentrations of chitosan solutions can be made, as long as the chitosan is fully dissolved), stirred for 2 minutes, left at room temperature for 2-4 hours, filtered with suction, discarded the residue, and the supernatant was passed through the treated polyamide resin column overnight. (The column volume is about 300mL, the flow rate is 300mL / hr), and the effluent is detected by FeCl3 reaction to confirm whether the resin is s...
Embodiment 2
[0073] 1. Preparation of high-purity extract of Apocynum apocynum
[0074] Take 300g of dried white hemp leaves, and extract 3 times with 60% ethanol water under reflux (3750, 2700mL, 2400) at a temperature of 85°C-100°C, each time for 1hr, combine the extracts, and let stand overnight at room temperature, Filtrate, remove the precipitate, concentrate the filtrate under reduced pressure (60°C, 0.08-0.1MPa) to a total volume of 900mL, add 180mL of 1% chitosan acetic acid solution to the concentrated solution, then add 180mL of 1% gelatin solution and stir for 2min at room temperature Stand still for 2-4hr, filter with suction, discard the residue, pass the supernatant overnight through a treated polyamide resin column (column volume about 300mL, flow rate 300mL / hr), and detect the effluent with FeCl3 reaction to confirm whether the resin is saturated; then Carry out elution with 600mL10% ethanol, eluent is discarded, then with 60% ethanol and acetone aqueous elution, elution is...
Embodiment 3
[0088] 1. Preparation of high-purity extract of Apocynum apocynum
[0089] Take 300g of dried Apocynum venetum leaves, extract 3 times (3000mL, 2700mL, 2400mL) with 45% ethanol water under reflux at a temperature of 85°C-100°C, 1hr each time, combine the extracts, let stand overnight at room temperature, filter , to remove precipitation, and concentrate the filtrate under reduced pressure (60°C, 0.08-0.1MPa) to a total volume of 900mL, add 160mL of 1% ZTC solution component A to the concentrated solution, and then add 160mL of 1% ZTC solution B component and stir for 2min at room temperature Stand still for 2-4hr, filter with suction, discard the residue, pass the supernatant overnight through a treated polyamide resin column (column volume about 300mL, flow rate 300mL / hr), and detect the effluent with FeCl3 reaction to confirm whether the resin is saturated; then Elute with 900mL of water, discard the eluent, then elute with 85% aqueous methanol until there is no AlCl3 reacti...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com