The invention discloses an HPLC method for determining the content of 6 components of Mango Zhike Tablets by one measurement and multiple evaluations. The invention adopts the HPLC method, and the chromatographic column is a Phenomenex Gemini C18 column (4.6 mm×250 mm, 5 μm); the mobile phase is acetonitrile ( B)-0.1% phosphoric acid aqueous solution (A); gradient elution; flow rate 1.0mL / min; detection wavelength: 258nm, column temperature 30°C; one-measurement-multiple-evaluation method takes gallic acid as the internal reference, and establishes protocatechuic acid, protocatechuic acid, The relative correction factor (RCF) of mangiferin, homomangiferin, hypericin and isoquercitrin is calculated by RCF, and its measured value is compared and analyzed with the measured result of external standard method. There is no significant difference between the measurement results of the test-multi-assessment method and the external standard method (P>0.05), and the relative correction factors have good repeatability. The high-performance liquid chromatography content determination and one-test-multi-assessment method established in the present invention can be used for Mango Zhike Tablets The content determination of the six components in the method is accurate and reliable, and the repeatability is good, which can be used for the quality control of mango cough tablets.