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Application of isoquercitrin as inhibitor of calcium ion channel

A calcium ion channel, isoquercitrin technology, applied in the application field of isoquercitrin, can solve the problem that there is no research showing the inhibitory effect of isoquercitrin

Active Publication Date: 2020-11-27
JIANGSU SUZHONG PHARM GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no research showing the inhibitory effect of isoquercitrin on TRPC channels, and in the preparation of TRPC channel-related cardiovascular diseases, coronary heart disease, atherosclerosis, end-stage renal failure, neurological diseases, chronic pain, acute pain or Use in medicine for inflammatory diseases

Method used

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  • Application of isoquercitrin as inhibitor of calcium ion channel
  • Application of isoquercitrin as inhibitor of calcium ion channel
  • Application of isoquercitrin as inhibitor of calcium ion channel

Examples

Experimental program
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Effect test

Embodiment 1-6

[0073] (1) Transfect the cDNA plasmid vector containing TRPC3, TRPC6 or TRPC7 ion channels into the HERK293 cell line, and then select the corresponding antibiotics to incubate the cells according to the resistance of the plasmid to selectively screen the successfully transfected cells. Functional tests were performed on the surviving cells to confirm the expression and function of the channel protein, and then cloned and purified through the "limited dilution" process to obtain a stable cell line that stably expresses a specific channel.

[0074] (2) Add 10-13×104 cells / mL of the stable cells obtained in step (1), and add 150 μL of cell suspension per well into a 96-well plate with a black wall bottom. After 24 hours of cultivation in the incubator, it can be used for subsequent experiments.

[0075] (3) Observe the cells, and after confirming that the cells are in good condition, load the dye (fluo-4) for 60 min.

[0076] (4) prepare channel activator and inhibitor solution...

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Abstract

The invention relates to application of isoquercitrin as an inhibitor of a calcium ion channel, and belongs to the field of medicine. The invention also provides application of the isoquercitrin in preparation of drugs for treating cardiovascular diseases, coronary heart diseases, atherosclerosis, advanced renal failure, neurological diseases, chronic pain, acute pain or inflammatory diseases. Theinvention proves that the isoquercitrin can inhibit the calcium ion channel, especially a TRPC ion channel, and expands the application of the isoquercitrin.

Description

technical field [0001] The technology of the invention belongs to the field of medicine, and in particular relates to the application of isoquercitrin. Background technique [0002] Cardiovascular disease is the most harmful disease to human life and health in modern society. According to the report of the World Health Organization, about 17.9 million people died of cardiovascular disease in the world in 2016, accounting for 31% of the total global deaths. It can be seen that the medical needs of new drugs for the prevention and treatment of cardiovascular diseases are very urgent. Relevant studies have proved that TRPC channels are important pharmacological targets for the development of new drugs for some cardiovascular diseases such as cardiomyopathy, heart failure, hypertension and cerebrovascular diseases. Patent application WO2006 / 074802A1 discloses that TRPC channels are used in the treatment of cardiovascular and cerebrovascular diseases. Its research shows that usi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K36/185A61P9/00A61P9/10A61P13/12A61P25/00A61P29/00
CPCA61K31/7048A61K36/185A61P9/00A61P9/10A61P13/12A61P25/00A61P29/00
Inventor 唐海涛曹征宇余伯阳葛海涛王正俊种法政
Owner JIANGSU SUZHONG PHARM GRP CO LTD
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