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Kindir leaf total flavone extract and its prepn and application

A technology of total flavonoids and extracts, which is applied in the application field of drugs for the treatment of cardio-cerebral ischemia, can solve the problems of unreached, unseen cerebral ischemic diseases, and unreachable second-class raw materials for oral use, and achieves reliable therapeutic effect

Inactive Publication Date: 2007-03-21
周亚球 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extract formed after collecting 40-70% of the eluate and concentrating is the effective part, and the total flavonoid content is 50-60%. The total flavonoid content of the active part is 50-60%, but it has not yet reached the standard of the second-class API for intravenous injection, that is, more than 80%
The problem with this technical solution is that although the contents of hyperin, total flavonoids, and hydrolyzed quercetin are provided, it is not clear which flavonoid components the extract consists of, and the quality of the extract cannot be fully reflected; The total flavonoids (calculated as hyperin) content of the extract is 42.8%, which has neither reached the standard of the second-class raw material drug for intravenous injection, that is, more than 80%, nor reached the standard of the second-class raw material drug for oral administration. , i.e. more than 50%
[0008] The traditional Chinese medicine Apocynum and its preparations are currently clinically used to treat hypertension, but there is no report on the treatment of cerebral ischemic diseases.

Method used

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  • Kindir leaf total flavone extract and its prepn and application
  • Kindir leaf total flavone extract and its prepn and application

Examples

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preparation example Construction

[0062] For the preparation of apocynum leaf total flavonoids extract, the specific operation process is as follows:

[0063] 1. Grind the dried Apocynum leaves into coarse powder, heat and reflux with 8-10 times the amount of 30-95% ethanol aqueous solution to extract for 1-2 hours, and extract 2-3 times in total;

[0064] 2. Concentrate the extract under reduced pressure at a temperature lower than 60°C to an extract with a specific gravity of 1.13-1.16 at 60°C, add 3-5 times the amount of the original drug in boiling water, dissolve in stages, and let stand for 24 hours to filter;

[0065] 3. The filtrate is chromatographed on a polyamide column, first washed with water for 8-12 column volumes, and then eluted, collected, and concentrated in sections with alcohol solutions of different concentrations. The operation process is as follows:

[0066] Use 40-50% ethanol aqueous solution or 40-50% methanol aqueous solution to elute for 3 column volumes, take every 0.5 column volum...

Embodiment 1

[0070] Get 1 kg of coarse powder of Apocynum apocynum leaves, add 10 times the amount of 80% ethanol aqueous solution and heat and reflux for extraction for 2 hours, extract 3 times altogether, combine the extracts, let cool, filter, and reclaim ethanol under reduced pressure until there is no alcohol. And concentrated to 60 ℃ extract with a relative density of 1.13; add boiling water equivalent to 5 times the amount of the crude drug, stir to dissolve in several times, filter, add the filtrate to the polyamide column treated according to the conventional method, and wash 10 columns with water After volume, 2 column volumes were eluted with 10% ethanol aqueous solution, 3 column volumes were eluted with 50% ethanol aqueous solution, 2 column volumes were eluted with 70% ethanol aqueous solution, and 50% ethanol aqueous solution was collected in sections, every 0.5 column volume The eluent is 1 part, collect the 2nd-4th part of eluent; the 70% ethanol aqueous solution and the 5t...

Embodiment 2

[0074] Take 1 kg of kenaf leaf crude drug powder, add 8 times the amount of 70% ethanol aqueous solution, heat and reflux for extraction for 2 hours, extract 3 times in total, combine the extracts, let cool, filter, recover ethanol under reduced pressure until there is no alcohol, and concentrate to 60 Extraction with a relative density of 1.14 °C; add boiling water equivalent to 4 times the amount of the crude drug, stir in several times to dissolve, filter, add the filtrate to a polyamide column that has been processed according to a conventional method, wash 10 column volumes with water, and use 10% ethanol aqueous solution eluted 1 column volume, 50% ethanol aqueous solution eluted 3 column volumes, 80% ethanol aqueous solution eluted 2 column volumes, collected 50% ethanol aqueous solution in sections, and eluted every 0.5 column volumes The solution is 1 part, collect the 2nd-4th eluate; the 80% ethanol aqueous eluate and the 5th and 6th eluent of 50% ethanol aqueous solu...

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Abstract

The present invention is kinder leaf total flavone extract and its preparation process and application, and features that the kinder leaf total flavone extract contains total flavone in 50-95 %, including rutin 0.2-30.0 %, hyperin 0.5-30.0 %, isoquercitrin 10.0-30.0 %, trifolin 3.0-20.0 %, astragaloside 2.0-5.0 %, 6''-O-acetyl isoquercitrin 1.0-6.0 %, quercitrin 0.5-4.0 %, trifolitin 0.2-3.0 %, etc. The kinder leaf total flavone extract is applied in preparing medicine for treating cardiac and cerebral ischemia.

Description

Technical field: [0001] The invention relates to an extract of total flavonoids from the leaves of Apocynum apocynum, a preparation method of the extract, a traditional Chinese medicine preparation, and an application thereof in drugs for treating heart and brain ischemia. Background technique: [0002] There are three kinds of Apocynum venetum leaf resources in my country: Apocynum venetum L. (Apocynum venetum L.) is also called kenaf, Poacynum hendersonni (Hook.f.) Wodson] and Apocynum venetum L. The plant Poacynum pictum (Schrenk) Baill, collectively referred to as Apocynum pictum (Schrenk) Baill, belongs to different genera of plants, and their flavonoid components are also different, and there is no comparison between them. The present invention relates to apocynum venetum leaves (Apocynum venetum L.), including kenaf leaves (Apocynum venetum L.) produced in Xinjiang. [0003] At present, the methods of studying the active ingredients of Apocynum can be summarized into ...

Claims

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Application Information

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IPC IPC(8): A61K36/24A61K31/7048A61P9/10C07H1/08
Inventor 王先荣周亚球刘东平
Owner 周亚球
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