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Actinidia valvata leaf general flavones extractive as well as preparation method and application thereof

A technology for treating calyx kiwi fruit leaves and extracts, which is applied in the field of medicine, can solve the problems of high preparation cost of monomer components, complicated processes, etc., and achieves the effects of significant anti-myocardial ischemia, simple process and high extraction yield.

Active Publication Date: 2015-07-22
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high cost and complicated process of preparation of monomer components, it is necessary to carry out research on the extract of Actinidia calyxa leaves in order to obtain an active extract with significant anti-myocardial ischemia effect

Method used

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  • Actinidia valvata leaf general flavones extractive as well as preparation method and application thereof
  • Actinidia valvata leaf general flavones extractive as well as preparation method and application thereof
  • Actinidia valvata leaf general flavones extractive as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of total flavonoids from kiwifruit leaves (1)

[0044] 2 kg of kiwifruit leaf medicinal material, 15 times, 10 times, 10 times of 40% ethanol aqueous solution as solvent, reflux extraction 3 times, extraction time 1.5 hours, 1 hour, 1 hour respectively, extract while hot filtered, combined extraction Liquid, the solvent is recovered under reduced pressure until there is no alcohol smell, the relative density is adjusted to 1.05g, it is adsorbed by HPD300 macroporous resin, the impurities are washed away with 6 times of column bed volume 20% (V / V) ethanol aqueous solution, and then 6 times of column bed volume Volume 60% (V / V) ethanol aqueous solution was eluted, collected eluate, concentrated under reduced pressure, dried, obtained total flavonoid extract 76.3g, detected by HPLC method, kaempferol-3-O-α-L-mouse The contents of lysyl-(1→3)-α-L-rhamnosyl-(1→6)-β-D-galactopyranoside, actin E and F were respectively 8.12%, 32.14%, 32.50%.

Embodiment 2

[0045] Example 2 Preparation of total flavonoids from kiwifruit leaves (2)

[0046] 2 kg of kiwifruit leaf medicinal material, use 50 times of 40% ethanol aqueous solution as solvent, extract by percolation, combine the extracts, recover the solvent under reduced pressure until there is no alcohol smell, adjust the relative density to 1.05, and absorb it with HPD300 macroporous resin to 6 Wash away impurities by twice column bed volume with 20% (V / V) aqueous ethanol solution, then elute with 6 times column bed volume with 60% (V / V) aqueous ethanol solution, collect the eluate, concentrate under reduced pressure, and dry to obtain total flavonoids Extract 76.3g, detected by HPLC method, kaempferol-3-O-α-L-rhamnosyl-(1→3)-α-L-rhamnosyl-(1→6)-β- The contents of D-galactopyranoside, actin E and F were 7.84%, 31.85%, and 33.69%, respectively.

Embodiment 3

[0047] Example 3 Preparation of total flavonoids from kiwifruit leaves (3)

[0048] The extraction step uses 95% aqueous ethanol as the extraction solvent, and the rest are the same as in Example 1 to obtain 32.4 g of the total flavonoid extract, which is detected by HPLC method, kaempferol-3-O-α-L-rhamnosyl-(1→ 3) The contents of -α-L-rhamnosyl-(1→6)-β-D-galactopyranoside, actin E and F were 3.12%, 16.33%, and 17.24%, respectively. %.

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PUM

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Abstract

The invention relates to an actinidia valvata leaf general flavones extractive as well as a preparation method and an application thereof. The preparation method comprises the steps of preparing an extracting solution, concentrating, edulcorating, drying and smashing. The preparation method has the advantages of high extracting yield of kaempferol-3-O-alpha-L-rhamnose-(1-3)-alpha-L-rhamnose-(1-6)-beta-D-pyran galactoside, actinidia valvata glycoside E and F, simple process, low cost, safety and effectiveness, and low organic solvent residue. The pharmacological experiment shows that the actinidia valvata leaf general flavones extractive has obvious anti-myocardial ischemia effect and can be used for preparing a medicine, a healthcare product and a food for resisting the myocardial ischemia related symptoms.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an extract of total flavonoids from Actinidia calyxae leaves and its preparation method and application. Background technique [0002] Myocardial ischemia refers to the clinical state in which coronary blood flow decreases due to various reasons, resulting in insufficient supply of myocardial oxygen and other substances and reduced clearance of metabolites. Clinically, the main and most common cause of myocardial ischemia is coronary artery (occasionally pulmonary artery) stenosis, and the main cause of coronary artery stenosis is atherosclerosis, and heart disease caused by coronary atherosclerosis is coronary artery disease. Heart disease, so it can be said that coronary heart disease is the "culprit" of myocardial ischemia. Myocardial ischemia may have many adverse effects on the heart and the whole body. Oxygen is an essential substance for the activity of cardiomyocytes, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A23L1/29A23L1/212A61P9/10A23L19/00A23L33/00
Inventor 辛海量
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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