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Total giamt St.John' swort Herb flavone extract, its preparing method and use

A technology for total flavonoids and extracts, which is applied in the field of total flavonoid extracts of Eclipta chinensis, can solve the problems of unsuitability for total flavonoids, and cerebral ischemic diseases have not yet been seen, and achieves the effect of reliable curative effect.

Inactive Publication Date: 2006-08-23
周亚球 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is a laboratory method for studying a single flavonoid component, and is not suitable for the preparation of total flavonoids
The traditional Chinese medicine Eclipta chinensis and its preparations are currently clinically used to treat bronchitis and cough, but there is no report on the treatment of cerebral ischemic diseases.

Method used

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  • Total giamt St.John' swort Herb flavone extract, its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Take 1 kg of crude drug powder of Eclipta chinensis, add 10 times the amount of 70% ethanol, heat and reflux for extraction for 2 hours, extract 3 times in total, combine the extracts, let cool, filter, recover ethanol under reduced pressure until there is no alcohol, and concentrate to the extract with a relative density of 1.15 (60°C); add boiling water equivalent to 4 times the amount of the crude drug and stir to dissolve, filter, add the filtrate to the treated polyamide column, wash 10 column volumes with water, and use 10% Elute with ethanol for 2 column volumes, and then elute with 70% ethanol until the eluate has no flavonoids, collect the 70% ethanol eluate, concentrate under reduced pressure at a temperature lower than 60°C, and dry in vacuum to obtain Eclipta chinensis Total flavonoid extract, yield 1.55%, its total flavonoid content is 83.20%, rutin 10.35%, hyperoside 21.52%, isoquercitrin 25.61%, quercetin 4.85%, kaempferol 1.52% , and several other flavon...

Embodiment 2

[0069] Take 1 kg of crude drug powder of Eclipta chinensis, add 8 times the amount of 80% ethanol, heat and reflux for extraction for 2 hours, extract 3 times in total, combine the extracts, let cool, filter, recover ethanol under reduced pressure until it is free of alcohol, and concentrate to an extract with a relative density of 1.15 (60°C); add boiling water equivalent to 5 times the amount of the crude drug and stir to dissolve, filter, add the filtrate to the treated polyamide column, wash 10 column volumes with water, and use 10% Elute with ethanol for 2 column volumes, then elute with 80% ethanol until the eluate has no flavonoids, collect the 80% ethanol eluate, concentrate under reduced pressure at a temperature lower than 60°C, and dry in vacuum to obtain the total Flavonoid extract, the yield is 1.65%, its total flavonoid content is 82.50%, rutin 10.60%, hyperoside 21.02%, isoquercitrin 25.05%, quercetin 5.05%, kaempferol 1.43%, and several other flavonoids.

Embodiment 3

[0071] Take 1 kg of crude drug powder of Eclipta chinensis, add 10 times the amount of 90% ethanol, heat and reflux for extraction for 2 hours, extract 3 times in total, combine the extracts, let cool, filter, recover ethanol under reduced pressure until it is free of alcohol, and concentrate to the extract with a relative density of 1.15 (60°C); add boiling water equivalent to 4 times the amount of the crude drug and stir to dissolve, filter, add the filtrate to the treated AB-8 or HPD100 macroporous adsorption resin column, and rinse with water for 10 column volume, eluted with 10% ethanol for 2 column volumes, and then eluted with 70% ethanol until the eluate was free of flavonoids, collected the 70% ethanol eluate, and concentrated under reduced pressure below 60°C, The total flavonoid extract of Eclipta chinensis was obtained by vacuum drying with a yield of 2.15% and a total flavonoid content of 58.24%. , 6.15% rutin, 14.62% hyperoside, 17.37% isoquercitrin, 3.54% querce...

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PUM

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Abstract

The present invention features that the extract has total flavone content of 50-90 wt%, and the total flavone consists of rutin 5.0-15.0 wt%, hyperoside 8.0-25.0 wt%, isoquercitrin 10.0-30.0 wt%, quercetin 2.0-7.0 wt% and kaempferol swartziol 0.8-2.5 wt% except other flavone components. The extract may be used in preparing medicine for treating cardiac and cerebral vascular diseases.

Description

Technical field: [0001] The invention relates to an extract of total flavonoids from Eclipta chinensis, a preparation method and application of the extract. Background technique: [0002] According to literature reports, Eclipta is the dry aboveground part of Hypericum ascyron L, a plant of the genus Hypericum in the family Garcinia, and its functions and indications are calming the liver, cooling blood to stop bleeding, clearing away heat and detoxifying. It is used to treat bleeding, bruises, sores, furuncles, swelling and poisoning, etc., and it is recorded in the standards of traditional Chinese medicines in Jiangsu Province. Eclipta contains flavonoids such as rutin, hyperin, isoquercitrin, quercetin and kaempferol. The extraction method of flavonoids can be summarized as follows: first extract with water or alcohol, dissolve the extract after recovering the solvent in water, extract with chloroform, ethyl acetate, and n-butanol respectively, divide it into several ext...

Claims

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Application Information

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IPC IPC(8): C07H15/00C07H17/00C07H1/08C07D311/30A61K31/70A61K31/33A61K9/14A61K9/08A61K9/20A61K9/48A61P9/10
Inventor 王先荣周亚球周光姣周丽
Owner 周亚球
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