The invention belongs to medical apparatuses and instruments for veterinary use, and aims at providing a disposable cervical cell brush for dogs and cats. The disposable cervical cell brush comprises a spherical guide head, a cell brush head, a sampling rod and a protection sleeve. Bristles, arranged in a spiral mode, of the cell brush head are perpendicular to the sampling rod, so that the whole cell brush head is in a cylindrical shape. One end of the cell brush head is inserted into the spherical guide head. The other end of the cell brush head is embedded in the sampling rod. The end, close to the cell brush head, of the sampling rod is provided with an annular groove. The tail end of the sampling rod is provided with a handle shaped like a fishtail. The protection sleeve is arranged outside the sampling rod. The disposable cervical cell brush has the advantages that the whole spherical face of the spherical guide head is smooth, so that it is ensured that the cell brush can not damage the vaginal wall in the process of entering and leaving the vagina and in the process of sampling; the protection sleeve is arranged outside the cell brush and the sampling rod for protection, on one hand, it can be ensured that no damage is caused to the vaginal wall in the process of entering and leaving the vagina, and on the other hand, contamination which is caused to the cell brush by the vulva can be avoided; the disposable cervical cell brush is safe, accurate, free of trauma, free of requirements for devices, wide in application range, and the like.