The invention discloses a liquid
mass spectrometry method for detecting various amino acids in
human blood plasma in an underivatized mode. The method includes the following steps that firstly, the
human blood plasma is obtained and subjected to deproteinization with
sulfosalicylic acid, and supernate is obtained; secondly, the supernate is mixed with an
internal standard solution containing a volatile ionized
reagent, LC-MS / MS is used for analysis, wherein according to chromatographic conditions, 1 mM perfluoroheptanoic acid
aqueous solution serves as a mobile phase A, a 1 mM perfluoroheptanoic acid
acetonitrile solution serves as a mobile phase B, and
gradient elution is conducted; according to
mass spectrum conditions, multi-
ion reaction monitoring of positive
ion electrospray ionization is conducted. A volatile ionized
reagent sample is added to a sample and mobile phase
system, analysis time is short, analysis efficiency is improved, operation is easy, and accuracy of experiment results is improved. LC-MS / MS is used for detecting twenty amino acids in the
human blood plasma, specificity, sensitivity and flux are high, operation is easy,
sample processing is easy, detection time is short, and the method is particularly suitable for clinical application and popularization.