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Reagent for measurement of leukocytes and hemoglobin concentration in blood

a technology for hemoglobin concentration and leukocyte count, which is applied in the direction of instruments, chemical methods analysis, material analysis, etc., can solve the problems of insufficient stabilizing effect of these stabilizers, affecting the measurement accuracy of leukocyte count, and affecting the quality of blood samples, etc., to achieve the effect of measuring the hemoglobin concentration stably and measuring the leukocyte coun

Inactive Publication Date: 2003-06-03
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

An object of the present invention is to provide a reagent which does not use a cyanogen compound, can measure the leukocyte count, and can also measure the hemoglobin concentration stably even when the liquid temperature of the sample varies.
The use of the reagent for measurement of leukocytes and hemoglobin concentration according to the present invention enables leukocytes and hemoglobin concentration to be measured simultaneously or separately.

Problems solved by technology

However, harmful cyanogen compounds are contained in the reagents.
The above hemolytic agents can denature hemoglobin rapidly, but have the drawback that the hemoglobin concentration markedly varies with the liquid temperature of the blood sample.
The stabilizing effect of these stabilizers is insufficient in this case.
This is one of the factors leading to an increase in the cost of the apparatus.

Method used

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  • Reagent for measurement of leukocytes and hemoglobin concentration in blood
  • Reagent for measurement of leukocytes and hemoglobin concentration in blood
  • Reagent for measurement of leukocytes and hemoglobin concentration in blood

Examples

Experimental program
Comparison scheme
Effect test

formulation example 2

Lauryltrimethylammonium chloride 0.3 to 10.0 g / L Stearyltrimethylammonium chloride 0.1 to 1.0 g / L EDTA-2K 0.5 to 5.0 g / L Good buffer 15 to 30 mM NaCl Proper amount Purified water 1 L

formulation example 3

Lauryltrimethylammonium chloride 0.3 to 10.0 g / L Lauryldimethylaminoacetate betaine 0.5 to 5.0 g / L EDTA-2K 0.5 to 5.0 g / L Succinate buffer 15 to 30 mM NaCl Proper amount Purified water 1 L

With Formulation Examples 1 to 3, leukocyte and hemoglobin can be measured simultaneously. By adjusting the type and concentration of each surfactant, leukocytes can be classified into two or more populations. The populations of leukocytes refer, for example, to lymphocytes, monocytes, neutrophils, eosinophils and basophils. Formulation Example 1 is a combination of the components designed to classify leukocytes into a single population, while Formulation Examples 2 and 3 are combinations of the components designed to classify leukocytes into two or three populations.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a graph showing the correlation between the hemoglobin concentration measured by the use of the reagent of the present invention and the hemoglobin concentration measured by a conventional met...

example 1

Stability to Changes in Temperature

The reagent of the present invention and reagents as Controls 1 and 2 to be described below were prepared. Each of the reagents was reacted with a patient's sample at a different temperature, and the hemoglobin concentration was measured.

Reagent of the Present Invention Lauryltrimethylammonium chloride 3.0 g / L Stearyltrimethylammonium chloride 0.2 g / L EDTA-2K 1.0 g / L phosphate buffer 20 mM (pH 5.0) NaCl Proper amount (the proper amount is such an amount that the electric conductivity is about 13 mS / cm) Purified water 1 L Control 1 Lauryltrimethylammonium chloride 3.0 g / L Stearyltrimethylammonium chloride 0.2 g / L Phosphate buffer 20 mM NaCl Proper amount (the proper amount is such an amount that the electric conductivity is about 13 mS / cm) Purified water 1 L Control 2 Lauryltrimethylammonium chloride 3.0 g / L Stearyltrimethylammonium chloride 0.2 g / L Imidazole 1.0 g / L Tris-maleate buffer (such an amount that the electric conductivity is about 13 mS / c...

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Abstract

A reagent for measurement of leukocytes and hemoglobin concentration in the blood includes a cationic surfactant in an amount sufficient to lyse erythrocytes and denature hemoglobin, at least one of the following hemoglobin stabilizers:(a) sulfosalicylic acid, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin,(b) 0.2 to 10.0 g / L of a water-soluble chelating agent having a nitrogen atom and a carboxyl group, and(c) piperazine, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, anda buffer for maintaining pH at 4 to 6.

Description

BACKGROUND OF THE INVENTIONThis invention relates to a reagent for measurement of leukocytes and hemoglobin concentration in a blood sample.Measuring the leukocyte count and hemoglobin concentration in the blood is very important for the clinical diagnosis of leukemia, anemia, etc. or for monitoring the clinical course in patients.Currently, the leukocyte count and hemoglobin concentration can be measured in a short time by an automatic blood analyzer, and such an analyzer is in widespread use.Automatic blood analyzers can be roughly classified into an optical detection system for detecting light scattering or fluorescence, and an electric resistance detection system for detecting a change in impedance occurring when particles pass through an aperture. The latter system is superior in terms of ease of use. According to the electric resistance detection system, the measurement of the leukocyte count is performed by adding a hemolytic agent to a blood sample to lyse erythrocytes and p...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): G01N33/50G01N33/72G01N33/49
CPCG01N33/5094G01N33/721Y10T436/10Y10T436/107497Y10T436/25Y10T436/108331Y10T436/101666Y10T436/25125Y10T436/2525
Inventor UCHIHASHI, KINYAIKEUCHI, YOSHIROSHIRAKAMI, ATSUSHIHAMAGUCHI, YUKIO
Owner SYSMEX CORP
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