48 results about "Dioscorea oppositifolia" patented technology

The invention provides the application of a paris saponin (I) and a ramification of the paris saponin (I) in the preparation of a drug for remedying the tumor. The chemical name of the paris saponin (I) is 4-O-furan Arabian glycosyl-2-O-pyran buckthorn glycosyl-beta-D-pyran glucosyl dioscorea saponin, and the molecular formula of the paris saponin (I) is C44H70O16. The drug, which is prepared by the invention, also contains a drug excipient or carrier which is allowed by the preparation. The invention is mainly applied to prepare the drugs for remedying the lung cancer and the leukaemia and has strong anticancer effect, and the animal experiment testifies that the effect of resisting the growth of the cancer cells of the paris saponin (I) is obviously better than a chemotherapeutic drug of cisplatin which is in common clinical use at present. The toxic and side effect of the paris saponin (I) is light, and the experiment of cell culture in vitro testifies that the paris saponin (I) and the ramification of the paris saponin (I) do not have obvious toxic and side effect towards the growth of the hematopoietic cells of the normal person under the quantity of an antineoplastic and are better than the normal chemotherapeutic drug.

The invention relates to a molecular identification method of dioscorea collettii hook.f. which is a main confused origin of Chinese traditional medicine rhizoma dioscoreae hypoglaucae. The method is that ISSR-SCARs markers and an identification method for identifying dioscorea collettii hook.f. are found on the basis of the processes of the dioscorea collettii hook.f. and dioscorea hypoglauca palib which is the orthodox origin of Chinese traditional medicine rhizoma dioscoreae hypoglaucae such as total DNA extraction, PCR amplification, primer screening ISSE-SCARs amplification, testing and analysis, etc. Using the primers tdisclosed by the invention to carry out the PCR reaction can obtain amplified fragments with 960bp length, which do not have in the dioscorea hypoglauca palib, from the dioscorea collettii hook.f. A high specificity identification primer capable of identifying different origins of Chinese traditional medicine rhizoma dioscoreae hypoglaucae is schemed out according to the difference of the ISSE-SCARs amplified fragment, and can successfully carries out the high specificity PCR identification of the dioscorea collettii hook.f.

The invention discloses a method for detecting a medicinal composition for treating urinary system diseases. The medicinal composition comprises 200-400 parts by weight of rhizoma dioscoreae hypoglaucae, 30-90 parts by weight of grassleaf sweelfal rhizome, 100-200 parts by weight of liquorice, 50-120 parts by weight of combined spicebush root, 20-80 parts by weight of fried sharpleaf galangal fruit, 200-300 parts by weight of dodder, 80-160 parts by weight of tuckahoe and the like. In the quality controlling method of the composition, the contents of diosgenin and glycyrrhizic acid are determined by adopting high performance liquid chromatography; and the rhizoma dioscoreae hypoglaucae, the grassleaf sweelfal rhizome, the liquorice, the fried sharpleaf galangal fruit and the combined spicebush root are subjected to qualitative thin layer identification. The composition has the efficacies of separating clear and resolving turbidity, warming kidney and removing dampness through diuresisand is used for the situations of the unsmooth flow of qi in the kidney, no separation of the clear from the turbid, frequent urination, as well as ongoing whitish turbidity.

This invention provides a new technology of cleaning production that extract sapogenin from dioscorea zingiberensis wright. It uses rhizome of fresh dioscorea zingiberensis or fresh dioscorea nipponica that are 2 -3 perennials as raw material. Its technology is that: raw material-microwave processing-ultramicro-pulverization-dynamic state continuous extracting-rectification-hydrolization-crude sapogenin-dissolving adsorption for purification-rectification-crystallization-drying-depurating sapogenin. This invention provides a new technology that produces diosgenin. Its technology is advanced, investment is little, material expenditure is low, production yield is high, quality is satisfactory, production period is so short that extracting technology can complete in 20 hours. It has no pollution. Content of COD in the water is less than 300mg / l.

The present invention discloses total saponin extract of dioscorea nipponica stem and leaf and its extracting and refining process and medicine use, and belongs to the field of new Chinese medicine extract. The above-ground parts of Chinese medicine material dioscorea nipponica is processed through water decoction, elution in macroporous adsorption resin with water to no color, elution with 80-95 % concentration ethanol solution to collect eluate, and recovering ethanol to obtain one new kind of Chinese medicine material. The total saponin extract of dioscorea nipponica stem and leaf has the effect of treating coronary heart disease, angina pectoris, myocardial ischemia, hyperlipemia and other diseases.

An anti-tumor traditional Chinese medicine, a preparation method therefor, and uses thereof. This traditional Chinese medicine is prepared by leavening of Pleurotus sp. and dioscorea bulbifera, pangolin scales, rhubarb, oyster, stiff silkworm, kelp, selfheal, ligusticum wallichii, barbat skullcap, honeysuckle flower, oldenlandia diffusa, radix trichosanthis, rhizoma anemarrhenae scutellaria baicalensis, angelica sinensis psoralea corylifolia, lucid ganoderma, and coix seed.

A preparing method of dioscorea composita saponin successively comprises the following steps: (1) pretreating raw material, (2) extracting, (3) concentrating, (4) acidizing and hydrolyzing, (5) carrying out heat reflux tripping, and (6) crystallizing, and thus obtaining the dioscorea composita saponin. The preparing method of the dioscorea composita saponin adopts a condensation method to recycle ethanol, ethanol tail gas is recycled for reutilization, and thus zero emission is achieved; moreover, the consumption amount of ethanol in the entire production process is less than 1%, therefore, the ethanol consumption is reduced, and in addition, the environment is protected. At the same time, during the ethanol recycling process, hot water is formed after circulating cooling water is subjected to heat exchange with ethanol steam, the hot water flows back to a continuous countercurrent ultrasonic extraction machine and is used for heating the continuous countercurrent ultrasonic extraction machine, and thus waste heat of the ethanol steam is effectively utilized, and energy is saved. In addition, dioscorea composita can be used as a main ingredient of an animal feed after the saponin component is extracted, and thus the problem of dioscorea composita waste residue treatment is completely solved.

The invention belongs to the technical field of skin care, and particularly relates to a face-rejuvenating and skin-cleaning mask composition and a preparation method thereof. The face-rejuvenating and skin-cleaning mask composition comprises the following components in percentages by weight: 14-18% of polyhydric alcohol with 2-6 carbon atoms, 3-7% of a wetting agent, 2-6% of a first skin conditioner, 0.003-0.007% of a second skin conditioner, 0.08-0.18% of a third skin conditioner, 0.104-0.144% of an extract, 0.08-0.13% of a thickening agent, 0.08-0.14% of an emulsifying agent, 0.01-0.05% ofa chelating agent and the balance of water. According to the mask composition, a medicinal laminaria extract, a lactobacillus / soybean fermentation product extract and a dioscorea japonica root extractare taken as the core, and are compounded with a plurality of skin conditioners, the emulsifier, the chelating agent and the like, various components are synergistic, and the problems of skin dryness, excessive fat, coarse pores, dull luster, pachulosis, no elasticity and the like are solved by promoting cell growth, softening keratin, inhibiting the activity of tyrosinase in vivo, promoting collagen synthesis, strongly locking water and the like.

The invention discloses an establishment method of a Dioscorea alata L. tissue culture regeneration system. Dioscorea alata L. has an alternate name of winged yam, is a perennial twining liana and belongs to the dioscorea of the dioscoreaceae; the Dioscorea alata L. is rich in nutrition, bright in color, and excellent in taste, and is a food popular with people. The Dioscorea alata L. is drought-resistant, heat-resistant, high in yield potential, relatively high in starch content and short in growth period, and is an energy plant having great development potential. At present, the Dioscorea alata L. is mainly multiplied from underground tubes by virtue of vegetative propagation, and a great quantity of seed materials can be consumed; besides, the self-seedling cultivation form is possible to result in the degradation of the properties of the seed, the reduction of the quality and the decrease of the yield, and therefore, the establishment of the Dioscorea alata L. isolated culture regeneration system is necessary. The establishment method comprises the major process of explant sterilization treatment, inoculation into a germinating culture medium to induce the callus, callus multiplication culture, callus tissue differentiation into multiple shoots, and rooting and seedling growing culture.

The invention discloses a method for extracting dioscorea nipponica. The method comprises the following steps: in an extracting tank of a solvent circulation extraction double-effect condenser set or a solvent circulation extraction single-effect condenser set, performing coarse grinding on the dioscorea nipponica according to the ratio of 1g:(5-7)ml, immersing in the water for 10-14h, heating, refluxing for 3.5-4.5h when the refluxing speed is 1.3-1.5 times of the initial water adding amount / (3.5-4.5)h, collecting the extract solution, decompressing and condensing, cooling, adding ethanol, fully stirring, standing, filtering to remove the precipitate, decompressing the filtrate, and recovering the ethanol to obtain the dioscorea nipponica extract. By adopting the method, the extracting efficiency of the dioscin is high and the dioscorea nipponica material is sufficiently utilized. The dioscorea nipponica is extracted under the technological parameter so that 7.3-7.6mg of the dioscin can be obtained in per gram of the dioscorea nipponica medicinal material.

The invention discloses a production process for extracting diosgenin. The production process comprises the following steps: pretreatment of raw materials: removing hairy roots of fresh dioscorea opposita plants, cleaning to remove silt, cutting into sections with a thickness of 0.1-1 cm, cleaning with water, and separating starch from dioscorea opposita residues; solvent extraction: collecting the dioscorea opposita residues, and performing solvent extraction to obtain dioscin; hydrolyzing the dioscin; and extracting, purifying and recrystallizing the hydrolysate. The production process can solve problems of waste and environmental pollution in the current acid hydrolysis and gasoline extraction, and can recycle residues, and improve extraction rate and purity of diosgenin obtained by extraction.

The invention relates to a multifunctional food additive. Raw materials comprise, by weight, 1-5 parts of dioscorea, 2-15 parts of lxora chinensis, 2-15 parts of convallaria majalis, 1-5 parts of perila seeds, 1-5 parts of pueraria, 5-10 parts of tree peony bark, 1-5 parts of orange peel, 2-10 parts of oysters, 1-5 parts of radish seed and 1-5 parts of fructus cannabis. Preferably, the raw materials include, by weight, 3 parts of dioscorea, 15 parts of lxora chinensis, 20 parts of yam, 15 parts of convallaria majalis, 1 part of perila seeds, 3 parts of pueraria, 10 parts of tree peony bark, 3 parts of orange peel, 8 parts of oysters, 3 parts of radish seed and 3 parts of fructus cannabis. The invention further relates to a preparation method for the multifunctional food additive. The multifunctional food additive has the advantages that the food additive can adjust and clear blood vessels from the dietary angle, improve physical conditioning, ameliorate constipation and dredge blood vessels.

The invention discloses a hormone double-item controlled-release regulating patch and a preparation method thereof, and relates to the technical field of hormone regulating medicines. The hormone double-item controlled-release regulating patch comprisesa a PE film and a TPU film arranged on the PE film, wherein the TPU film is coated with a pressure-sensitive adhesive in advance and then coated with a regulating preparation layer, and the regulating preparation layer is covered with a release film, and comprises extract, gel, an emulsifier and glycerin. The extract is prepared from the following raw materials: radix paeoniae rubra, rhizoma sparganii, curcuma zedoary, frankincense, musk, bupleurum chinense, selaginella lepidophylla, endive, avocado flower, radix ginseng rubra, rhodiola rosea, herba epimedii, American soybean germ, Indian ginseng, Thailand pueraria mirifica and dioscin. The invention also discloses the preparation method of the hormone double-item controlled-release regulating patch, and the preparation method disclosed by the invention is easy to operate and beneficial to industrial popularization.

The invention provides a method for inducing transgenic hairy roots of dioscorea composita. The method comprises the steps of tissue culture seedling cultivation, preparation of transgenic infecting bacterium liquid, infection and co-culture, induction of hairy roots, sterilization culture of the hairy roots and liquid amplification culture of the hairy roots. According to the method for inducingthe transgenic hairy roots of the dioscorea composita, by taking root-removed tissue culture seedlings of the dioscorea composita as explants and agrobacterium rhizogenes C58C1 as an induction strain,the hairy roots can be efficiently induced under the condition of acetosyringone with specific concentration, and the induction rate can reach 50-85%; and meanwhile, the transgenic hairy roots containing different target genes can be induced according to requirements. The method is of great significance for identification of functional genes of the dioscorea composita, research on biological metabolic pathways of dioscin synthesis and the like, molecular breeding, industrial production of secondary metabolites and the like.

The invention discloses a liquid pollen food and a production method thereof. The liquid pollen food comprises 80-90% of rape pollen, 5-10% of corn pollen, 2-3% of Dioscorea nipponica Makino, 1-2% ofChlorella pyrenoidosa, 1-2% of Polyporus umbellatus, 2-5% of a Hippophae rhamnoides juice, 2-3% of Chaenomeles speciosa, 5-8% of mixed pollen, 2-4% of Thamnolia vermicularia and a proper amount of water. The food has the effects of strengthening the brain, detoxifying and protecting the skin, and can treat palpitation, assist sleeping, relieve cough and asthma and relieve stroke, and tests prove that the food has good antiviral and anti-inflammatory effects.

The invention relates to an organic composite fertilizer and a preparation method thereof. The organic composite fertilizer comprises Chinese medicine residues, dioscorea spongiosa, barks of fraxinusszaboana, borax, chlorite schist, a silicate bacterium fertilizer, plant ash, pumice powder, cellulose degradation bacterium colonies, polydimethylsiloxane, water soluble silicone oil, dicyandiamide,and water. The organic composite fertilizer is used as the basic fertilizer for cotton plantation. The Chinese medicine residues are degraded by cellulose degradation bacterium colonies; the utilization rate of the Chinese medicine residues is increased, the bacterium colonies also kill multiple plant pathogenic bacteria and insects; moreover, the nitrogen content of soil is increased, and the disease resistant performance of plants is enhanced. Dioscin in dioscorea spongiosa and scopoletin in barks of fraxinus szaboana further strengthen the insect killing effect. Chlorite schist contains many trace elements that are beneficial for plants and can be degraded and converted into substances that can be absorbed by crops by the silicate bacteria. The pumice can absorb ammonia and fix nitrogen. Dicyandiamide further reduces the loss of nitrogen. The using efficiency of the fertilizer is improved.

The invention discloses a high-yielding colletotrichum lini strain LJYH20130405-1 for steride fermentation as well as an application technology of the strain. A taxonomic status of the strain LJYH20130405-1 is that the sexual generation belongs to the fungi, ascomycota, sordariomycetes, sordariomycetidae, small nectriaceae and glomerella; the strain is preserved in (China General Microbiological Culture Collection Center on June 8th, 2013, with the preservation number of CGMCC 7715. According to the colletotrichum lini strain, 7alpha, 15alpha-dyhydroxyl androstenolone can be produced by using dehydroepiandrosterone (DHEA) as a substrate in a conversion process of producing plant hormones by using steroids as raw materials. The produced 7alpha, 15alpha-dyhydroxyl androstenolone has the characteristics of high conversion rate, high yield, comparatively stable biological properties and the like. According to the colletotrichum lini strain for steride fermentation, the technical support can be provided for improving the production yield of plant steroid hormone using dioscin as a raw material and reducing the cost.

The invention provides a rhizome crop harvesting method and device. The method comprises the following steps of: segmenting soil on two sides and the bottom of a crop rhizome, and forming a fracture surface around the crop rhizome; lifting soil which surrounds the crop rhizome; quickly lowering the lifted soil; and ensuring that the soil which surrounds the crop rhizome is loose, and pulling out the crop rhizome along the upper end of the crop rhizome or the plant of the crop rhizome. The method fully utilizes the characteristic that the soil in which the rhizome crops, including Dioscorea oppositifolia L. and the like are planted, is loose and fragile to integrally segment the soil which surrounds the Dioscorea oppositifolia L., then, the soil is quickly thrown, the sandy soil which is fragile is decomposed after being knocked, and at the time, the Dioscorea oppositifolia L. can be easily pulled out. Therefore, no furrows need to be dug, a vibration mechanism does not need to be arranged, a transmission mechanism with a complex structure is not used, an integral structure is simple, and manufacturing and repairing are simple and easy to implement.

The invention relates to the technical field of essential oil microcapsules, and provides an eupatorium adenophorum essential oil microcapsule, a preparation method thereof and an eupatorium adenophorum essential oil modified viscose fiber fabric. The eupatorium adenophorum essential oil microcapsule provided by the invention comprises a capsule wall and a capsule core, the capsule core comprises eupatorium adenophorum essential oil and a cosolvent; the capsule wall comprises whey protein isolate and yam polysaccharide. According to the present invention, the dioscorea alata polysaccharide mainly comprises glucose and mannose, the connection between the glucose and the mannose is mainly based on a beta-glucosidic bond, and a plurality of beta-1, 4-g mannose residues can form a spiral structure, such that the good embedding of the essential oil can be achieved by using the dioscorea alata polysaccharide as the wall material of the microcapsule; moreover, the dioscorea amabilis polysaccharide has good antibacterial performance, so that the microcapsule obtained by embedding the eupatorium adenophorum essential oil by taking the dioscorea amabilis polysaccharide as a wall material also has good antibacterial performance.

An herbal formula used for preparing a pharmaceutical composition for preventing and treating metabolic syndrome is provided. The herbal formula includes Rehmanniae radix preparata, Cornus officinalis, Dioscorea polystachya, Alisma plantago-aquatica, Paeonia suffruticosa, Poria cocos, Aconitum carmichaeli, and Cinnamomum cassia.

A pharmaceutical composition or a health food composition for controlling blood sugar, raw materials of which include Dioscoreae rhizome, medlar, and coconut oil. A preparation method for the composition includes: mixing Dioscoreae rhizome and a decoction thereof with medlar for homogenization, pulverization and drying, and mixing the resultant product with coconut oil and water, followed by baking and drying. Further disclosed are a pharmaceutical preparation or a health food including the composition and an excipient, and a use of the composition in the preparation of a pharmaceutical preparation or a health food for reducing or eliminating diabetic conditions.

The invention discloses a Chinese magnoliavine fruit beverage and a preparation method thereof. The Chinese magnoliavine fruit beverage comprises the following raw materials: wild Chinese magnoliavinefruits, wild honey and wild Chinese herbal medicine flowers, wherein the wild Chinese herbal medicine flowers comprise wild waterlilyleaf goldenray flowers, wild pueraria flowers, codonopsis pilosulaflowers, edible lily flowers, wild Chinese doughnut flowers, wild Chinese yam flowers, dioscorea zingiberensis flowers, paris polyphylla, epimedium sagittatum flowers and osmunda cinnamomea. The preparation method of the Chinese magnoliavine fruit beverage can be used for preparing Chinese magnoliavine fruit beverages according to any one of claims 1-4. The method is characterized by comprising the following steps: S1, taking the ripe fresh wild Chinese magnoliavine fruits, performing cleaning with clear water, performing draining, taking and cleaning the fresh wild Chinese herbal medicine flowers, putting the cleaned flowers into a steaming container, performing heating with water vapor, performing cooling, and performing drying in the sun; and S2, sealing a jar containing the drained wild Chinese magnoliavine fruits, the dried wild Chinese herbal medicine flowers and the wild honey for soaking and fermentation, opening the jar after 3-6 months, and performing sterilizing, filteringand canning to obtain the Chinese magnoliavine fruit beverage.