ISSR-SCARs marker for identifying main confusion matrix original dioscorea collettii hook.F. with chinese herb rhizoma dioscoreae hypoglaucae

Abstract

The invention relates to a molecular identification method of dioscorea collettii hook.f. which is a main confused origin of Chinese traditional medicine rhizoma dioscoreae hypoglaucae. The method is that ISSR-SCARs markers and an identification method for identifying dioscorea collettii hook.f. are found on the basis of the processes of the dioscorea collettii hook.f. and dioscorea hypoglauca palib which is the orthodox origin of Chinese traditional medicine rhizoma dioscoreae hypoglaucae such as total DNA extraction, PCR amplification, primer screening ISSE-SCARs amplification, testing and analysis, etc. Using the primers tdisclosed by the invention to carry out the PCR reaction can obtain amplified fragments with 960bp length, which do not have in the dioscorea hypoglauca palib, from the dioscorea collettii hook.f. A high specificity identification primer capable of identifying different origins of Chinese traditional medicine rhizoma dioscoreae hypoglaucae is schemed out according to the difference of the ISSE-SCARs amplified fragment, and can successfully carries out the high specificity PCR identification of the dioscorea collettii hook.f.

Description

Technical field: [0001] The invention belongs to the field of molecular marker technology and crude drug identification, and is a set of molecular identification methods for identifying the original plant of Chinese medicinal herba syringa and the main confusing original plant. Background technique: [0002] According to "The Pharmacopoeia of the People's Republic of China" (2005 edition), the base of traditional Chinese medicine powdered yam (Rhizoma Dioscoreae Hypoglaucae) is mainly Sect. Stenophora (D. collettii var.hypoglauca Pei et C.T.Ting.), the original variant of D. collettii Hook.f. is used in some places as a local custom of D. collettii. Because of the relationship of varieties, these two Seed base plants and medicinal materials are very similar in appearance, tissue structure and starch granule shape, and it is difficult to identify them by conventional methods, and their trnL-F, rcbL and matk sequence alignment differences are also small. [0003] Sequence-spe...

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Problems solved by technology

[0004] At present, there are no reports about ISSR-SCARs markers at home and abroad to identify the original plant powder Dioscorea chinensis and Dioscorea chinensis of the traditional Chinese medicine powder, and the present invention fills up the gap in this field

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