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Application of paris saponin I and its derivatives

A technology of papaya saponins and derivatives, applied in the field of papaya saponins derivatives, to achieve strong anti-cancer effects and light toxic effects

Active Publication Date: 2008-03-19
HANGZHOU BENSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research report on the application of saponin I and its derivatives in the preparation of drugs for the treatment of lung cancer and leukemia

Method used

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  • Application of paris saponin I and its derivatives
  • Application of paris saponin I and its derivatives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Determination of the inhibitory effect of papaya saponin I on the proliferation of human lung cancer cells.

[0018] (1) Experimental materials

[0019] Cell lines: Human lung cancer cell lines: A549, NCI-H460 and SK-MES-1 human cell lines were purchased from Shanghai Cell Bank, Chinese Academy of Sciences

[0020] Reagents: Papaya saponin I was purchased from China National Institute for the Control of Pharmaceutical and Biological Products

[0021] Instruments: cell incubator, microplate reader

[0022] (2) Experimental method

[0023] 8000 well-grown tumor cells were taken and inoculated into wells of a 96-well cell culture plate. The culture medium is DMEM high glucose cell culture medium containing 10% fetal bovine serum. Place carbon dioxide (5% CO2) cell incubator at 37 DEG C and cultivate after 24 hours, add different concentrations of papaya saponin I, after mixing, continue to put CO2 (5% CO2) cell culture chamber at 37 DEG C and cultivate for 7...

Embodiment 2

[0029] Example 2: Determination of the inhibitory effect of papaya saponin I on the proliferation of seven different human leukemia cell lines.

[0030] (1) Experimental materials

[0031] Cell lines: human leukemia cell lines: sensitive human chronic myeloid leukemia cell line K562-S and Gleevec-resistant K562-G01 cells, human monocytic leukemia cell lines: THP1 and U937, human myeloid myeloid leukemia KG -1. Human T lymphocytic leukemia cell line H9 and human B lymphocytic leukemia cell line Raji were purchased from Shanghai Cell Bank of Chinese Academy of Sciences.

[0032] Reagents: Papaya saponin I was purchased from China National Institute for the Control of Pharmaceutical and Biological Products

[0033] Instruments: cell incubator, microplate reader

[0034] (2) Experimental method

[0035] 8000 well-grown tumor cells were taken and inoculated into wells of a 96-well cell culture plate. The culture medium is RPMI-1640 cell culture medium containing 10% fetal bovin...

Embodiment 3

[0038] Example 3: Determination of the effect of papaya saponin I on the growth of normal human hematopoietic cells.

[0039] (1) Experimental materials

[0040] Normal human blood cells: Normal human blood cells are obtained from the peripheral blood of healthy volunteers

[0041] Reagents: Papaya saponin I was purchased from China National Institute for the Control of Pharmaceutical and Biological Products

[0042] Instruments: cell incubator, microplate reader

[0043] (2) Experimental method

[0044] Peripheral blood was drawn from healthy volunteers. Mononuclear cells were separated with Ficoll-Paque and suspended in RPMI-1640 medium (containing 10% fetal bovine serum), and the cell concentration was adjusted to 1×10 6 / ml, inoculated into 96-well cell culture plate, 0.2ml per well, added different concentrations of barley saponin I, put carbon dioxide (5% CO2) 37 ℃ cultured for 48 hours in a cell incubator. Then use the MTT method to measure the concentration of via...

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Abstract

The invention provides the application of a paris saponin (I) and a ramification of the paris saponin (I) in the preparation of a drug for remedying the tumor. The chemical name of the paris saponin (I) is 4-O-furan Arabian glycosyl-2-O-pyran buckthorn glycosyl-beta-D-pyran glucosyl dioscorea saponin, and the molecular formula of the paris saponin (I) is C44H70O16. The drug, which is prepared by the invention, also contains a drug excipient or carrier which is allowed by the preparation. The invention is mainly applied to prepare the drugs for remedying the lung cancer and the leukaemia and has strong anticancer effect, and the animal experiment testifies that the effect of resisting the growth of the cancer cells of the paris saponin (I) is obviously better than a chemotherapeutic drug of cisplatin which is in common clinical use at present. The toxic and side effect of the paris saponin (I) is light, and the experiment of cell culture in vitro testifies that the paris saponin (I) and the ramification of the paris saponin (I) do not have obvious toxic and side effect towards the growth of the hematopoietic cells of the normal person under the quantity of an antineoplastic and are better than the normal chemotherapeutic drug.

Description

technical field [0001] The present invention relates to papaya saponin derivatives, in particular to the application of paprika saponin I and its derivatives in the preparation of medicines for treating lung cancer, leukemia and other tumor diseases. technical background [0002] Lung cancer occurs in the epithelium of the bronchial mucosa, also known as bronchial carcinoma. According to statistics, lung cancer is one of the most common cancers in the world, with a very high mortality rate. One person dies from lung cancer every 30 seconds in the world, and there are 1.2 million new cases every year. Lung cancer is also the cancer with the highest incidence rate in my country, with an annual incidence rate of 35 per 100,000 people. Every day, more than 3,000 people worldwide die from lung cancer. Lung cancer is very hidden in the early stage, the symptoms appear late, and the cancer cells have often spread to other organs of the body. Only less than 5% to 15% of patients ...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P35/00
Inventor 徐荣臻于晓方张旭照范军强董爱强孔敏坚
Owner HANGZHOU BENSHENG PHARMA
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