148 results about "Dioscorea zingiberensis" patented technology

The invention relates to a method for fast detecting content of dioscin in dioscorea zingiberensis by utilizing near infrared spectrum technology, which effectively solves the problems that a traditional analysis method is complex, high in production cost, low in efficiency and plenty in raw material waste and comprises utilizing a near infrared spectrometer to scan near infrared absorbing spectrum of a calibration sample set, performing pre-treatment and spectrum area selection to obtain calibration sample set characteristic spectrum, and utilizing high performance liquid chromatography to detect index content of the calibration sample set dioscorea zingiberensis to serve as a reference value; utilizing a partial least squares method to combine the characteristic spectrum of the calibration sample set and the reference value to establish a calibration model, then substituting characteristic spectrum of a validation sample set into the calibration model, and obtaining a night-time ozone profile (NIR) predict value; comparing the NIR predict value with the content of validation sample set dioscorea zingiberensis detected through the high performance liquid chromatography (HPLC), performing validation on the calibration model, crushing a sample to be tested, scanning a near infrared spectrogram of the sample to be tested , and after performing pre-treatment and spectrum area selection, inputting the calibration model. The method for fast detecting the content of the dioscin in the dioscorea zingiberensis by utilizing the near infrared spectrum technology is short in time, fast in speed, accurate and capable of performing detection on line in real time, improving working efficiency and reducing cost.

The invention discloses a novel botanical insecticide and its preparation method and application and belongs to the field of insecticide preparation technology. The novel botanical insecticide comprises the following raw materials: ailanthus altissima, castor-oil plant, Chinese prickly ash, sabah veratrum nigrum, tubatoxin, garlic, tarragon, melia azadarach, onion, leaves of cinnamomum burm anii, ash bark, radix sophorae flavescentis, euphorbia soongarica, sophora alopecuroide, pyrethrum, dioscorea zingiberensis, lettuce, mint, wasabi, patrinia herb and water. The novel botanical insecticide is prepared by steps of medical liquid extraction, concentration, drying, crushing, sieving, sterilization and the like. The novel botanical insecticide has advantages of nontoxic component and no residue, is pollution-free, green and environmentally friendly, and will not cause environmental pollution. The novel botanical insecticide can effectively control plant insects such as inchworm, red spider, lopholeucaspis japonica, leaf miner and the like. One-time killing rate reaches 96.42% and above, and the plant insects have no drug resistance.

The invention provides a green extraction technology for the comprehensive and efficient utilization of a Dioscorea zingiberensis resource. Saponins are extracted and simultaneously other effective components in Dioscorea zingiberensis, such as Dioscoreae starch, celluloses, a coronary heart disease control raw material, pectin, agricultural nucleic acids and the like, are comprehensively, efficiently and synchronously extracted through the technology. Rhamnose is extracted from a hydrolysis waste liquid; the whole process of the technology is green; and the technology has the advantages of great improvement of the saponin production level in China, improvement of the resource utilization efficiency, energy saving, environmental protection, realization of the unification of the economic benefit, the environmental benefit and the social benefit, and promotion of the sustainable development of Chinese economy and society.

The invention provides a method for cleaning and producing dioscorea zingiberensis saponin with microbial technology adopted, and belongs to the field of bioengineering. The method solves the problems that in the existing dioscorea zingiberensis industry, wastewater and waste residues are large in drainage amount, and dioscorea zingiberensis starch is not utilized effectively. The method includes the following steps: releasing dioscorea zingiberensis saponin with microorganism adopted firstly, obtaining fermentation liquor containing the dioscorea zingiberensis saponin and the starch; and then processing the starch in the fermentation liquor with a two-enzyme method adopted and obtaining hydrolyzed sugar, and further releasing the dioscorea zingiberensis saponin; conducting solid-liquid separation on dioscorea zingiberensis mash; gathering the hydrolyzed sugar and the dioscorea zingiberensis saponin with microfiltration and nanofiltration membrane separation adopted in sequence, or obtaining intracellular products and dioscorea zingiberensis saponin through specific microbial fermentation separation, and obtaining the dioscorea zingiberensis saponin by hydrolysis of glycosidase and a small amount acid and extraction of a small amount of organic solvent on concentrated dioscorea zingiberensis saponin solution. According to the method, the yield coefficient of the dioscorea zingiberensis saponin is high (90-95%), wastewater quantity is little in the process of production, degree of contamination is low, microbial treatment is low in cost and short in use time, the dioscorea zingiberensis starch is recycled fully, and the method has a great industrial application promotion value in the dioscorea zingiberensis industry.

The invention discloses a method for remedying heavy metal contaminated soil by combining plants with microorganisms. The method comprises the specific steps that (1), the contaminated soil is deep ploughed 40 cm, then meshed pipelines with the diameter of 30-40 mm are buried, and sterilization and disinfection liquid is introduced into the pipelines; (2), a base fertilizer is applied, and a microorganism mixed fungicide and water are mixed according to the mass ratio of 1:5 and then sprayed according to the specification of 20-30 kg per mu; (3), dioscorea zingiberensis is planted, and mixed seeds of vetiver grass and bidens maximowicziana are sown between every two adjacent rows; and (4), the vetiver grass and the bidens maximowicziana which are planted among the dioscorea zingiberensis are rooted up to recycle heavy metal on September to October every year, the seeds are sown again on March to April in the next year, and the dioscorea zingiberensis, the vetiver grass and the bidens maximowicziana are rooted up together after three continuous years to recycle the heavy metal. The soil remediation method is good in remediation effect, low in cost, easy to manage and operate and capable of not generating secondary pollution and has a wide application prospect.

The invention discloses a Bacillus subtilis WB8 capable of producing diosgenin, and belongs to the technical field of applied microbiology. The strain is separated from internal issues of subterraneous stems of a medicinal plant of Dioscorea zingiberensis wright, can synthesize and secrete a metabolin of a host plant, namely diosgenin, and has the preservation number of CCTCC (China Center For Type Culture Collection) NO:M2010271; and the strain is rod-like, has the size of 1-1.5*3-5 mu m, forms a single-chain sporocarp, releases endospore, has rough colony with grid bulges, forms pellicle through liquid fermentation, has rich foam, and can grow 16SrDNA core sequence 1542bp with the thermophilic temperature of between 30 and 35 DEG C, the pH of 6.0-9.0 and capacity of tolerating 9.0 percent NaCl, wherein the core sequence has the sequence number of HM210636. The strain liquid is fermented by a common culture medium for 48 to 60 hours, the chloroform extract of the fermentation liquor is the diosgenin, and the diosgenin has the activity of inhibiting pathogenic bacteria and resisting tumor cells. The strain provided by the invention can produce the diosgenin; and compared with the conventional process of extracting the diosgenin from yam plants, the method avoids the pollution of strong acid and yam byproducts on environment, and has potential industrial application prospect.

The invention discloses a botanical insecticide for fruit trees and a preparation method and an application thereof, and belongs to the technical field of insecticide preparation. The botanical insecticide for fruit trees comprises the following raw materials: scutellaria baicalensis, purslane, datura stramonium, pyrethrum, paulownia leaf, sophora alopecuroide, magnolia cortex, melia azedarach, wasabi, chinaberry bark, ash bark, sophora flavescens, euphorbia soongarica, anabasis aphylla, dioscorea zingiberensis, lettuce, mint, tripterygium wilfordii, salix babylonica leaf, Chinese prickly ash and water. The botanical insecticide for fruit trees is prepared by steps of medical liquid extraction, concentration, drying, crushing, sieving, sterilization and the like. The botanical insecticide for fruit trees has advantages of nontoxic component and no residue, is pollution-free, green and environmentally friendly, and will not cause environmental pollution. The botanical insecticide for fruit trees can effectively control plant insects such as citrus bud mite, Aonidiella aurantii, papilionoidea, tortrix and the like. One-time killing rate reaches 95.56% and above. The plant insects have no drug resistance and can be used for 4-6 years.

The invention relates to a method for preparing a charcoal slow-release fertilizer by utilizing dioscorea zingiberensis saponin fiber waste residues. The method comprises the following steps of: (1) air drying the dioscorea zingiberensis saponin fiber waste residues, smashing and sieving; (2) putting in a reactor, rising temperature to be 300-600 DEG C, and carrying out pyrolytic carbonization under the condition of oxygen limited to obtain charcoal powder after cooling and grinding; and (3) spraying the charcoal powder onto binder solution, packaging a fertilizer for 2-5 times, air drying after mixing uniformly, or pelleting through a comminutor, or compression molding through a mould, so as to obtain the charcoal slow-release fertilizer. According to the method, the process is simple, the cost is low, and the time and the energy are saved; and charcoal and the fertilizer are packaged according to a certain proportion, and a complementary or synergistic effect which exists between the charcoal and the fertilizer is utilized, so that the nutrient efficiency is improved.

The invention discloses a biological organic silicon bacterial fertilizer capable of degrading heavy metals with high efficiency, and a preparation method thereof. The biological organic silicon bacterial fertilizer is prepared from a fermentation microorganism agent, a functional microorganism agent, an enzyme preparation, a rare earth silicon raw material, straw powder, an amino acid raw powder, abamectin residue, potassium fulvic acid, dioscorea zingiberensis residue, cassava residue, and livestock manure at a certain ratio. The preparation method comprises following steps: 1, cultivation of the activated fermentation microorganism agent; 2, cultivation of the activated functional microorganism agent; 3, fermentation preparation; 4, fermentation; and 5, fermentation postprocessing. The biological organic silicon bacterial fertilizer is capable of reducing heavy metal content of soil, providing plants with abundant trace elements, improving plant biological characters, resisting diseases and insects, and realizing nitrogen fixation, phosphorus dissolving, and potassium releasing. The preparation method is simple; operation is convenient; production cost is low; and the preparation method is suitable for popularization and industrialized large-scale production.

A preparation method of rubber gloves containing natural vanilla extracts is provided. The preparation method comprises the following steps: uniformly adding and dispersing certain amounts of vanilla powder, Dioscorea zingiberensis powder, aloe powder and other routine ingredients to natural latex or butyronityile latex in the prevulcanization operation of raw materials of routine natural latex gloves or butyronityile latex gloves; dipping according to a routine shaping process to prepare natural latex gloves containing the natural vanilla extracts or butyronityile latex gloves containing the natural vanilla extracts; carrying out operations of overturning, chlorination, steep-washing, rinsing, and drying to obtain the finished rubber gloves. The natural latex gloves and the butyronityile latex gloves produced through the method have antibiosis and skin care functions, and can emit a fragrant smell. An effect that sweat generated on hands of a wearer is reduced can be reached by further adding acrylic acid / acrylamide copolymerization phase change energy storage microcapsules.

The invention discloses a method for producing diosgenin through microbial conversion of peltate yam rhizome-yellow ginger. Yellow ginger dry powder and tap water are mixed according to a massic volume ratio of 1:5-12, urea which is 4.0% of mass of the yellow ginger dry powder, yeast extract which is 1.0% of mass of the yellow ginger dry powder and inorganic salt which is 0.2% of volume of the tap water are supplemented to prepare conversion slurry, the conversion slurry is introduced in bacillus bacterial strain XBT 2011 seed liquid which is 5% of the volume of the tap water, under the condition that the culture temperature is 40 DEG C, the pH is controlled to be about 6.0, the stirring speed is 300-400rpm and the ventilation condition is 2.5-4.0l / min, the conversion is 56-72hrs, conversion slurry is filtered and dried to obtain hydrolysate, the hydrolysate is extracted for 12-16hrs by using 120# gasoline, the volume of the 120# gasoline is one time of that of the hydrolysate, and diosgenin end products are obtained through repeated crystallization. A preservation number of the bacterial strain XBT 2011 is CGMCCNo.6301. The dry weight of the hydrolysate is 28-33% of that of the added yellow ginger dry powder, total saponins of the peltate yam rhizome is approximately converted into diosgenin completely, handling capacity is big, no sugar contains, the pH is close to neutral, and environmental pollution is reduced.

The invention disclose a method for extracting dioscorea zingiberensis pigment by an enzymatic method, which comprises the following steps of: dissolving pectinase in an acetic acid-sodium acetate solution to prepare a pectinase enzymatic solution; adding dioscorea zingiberensis powder into the pectinase enzymatic solution, then adding the acetic acid-sodium acetate solution for reacting, and adding anhydrous ethyl alcohol; extracting in thermostatic waterbath with the temperature of 65-70 DEG C; repeatedly extracting; merging filtrate; and then concentrating the filtrate to that the concentration of ethyl alcohol is less than 10 percent to obtain the dioscorea zingiberensis pigment. By utilizing the special property of the pectinase, the invention effectively improves pigment extraction amount without environmental pollution during production and lays a foundation for the scale production of the dioscorea zingiberensis pigment. By adopting enzyme to pretreat the dioscorea zingiberensis for damaging cell structures and degrading impurities, the invention has higher pigment obtaining efficiency and pigment quality, and not only alleviates environmental pollution and improves the utilization rate of the dioscorea zingiberensis, but also explores a novel edible natural pigment.

The invention relates to a traditional Chinese medicine for treating hidradenitis suppurativa. The traditional Chinese medicine is prepared from the following medicinal materials in parts by weight: 15 parts of mucuna paohwashanica, 14 parts of ficus hispida L. f., 14 parts of colocasia antiquorum schott, 10 parts of begonia palmata, 12 parts of celastrus angulata maxim, 12 parts of crotalaria albida heyne, 12 parts of rabdosia, 12 parts of white gentian, 14 parts of anemone rivularis buch, 15 parts of oxalis rubra, 8 parts of piper wallichii, 15 parts of hypericum uralum, 12 parts of claoxylon polot merr, 10 parts of anemome tomentosa, 10 parts of Ixeris polycephala, 5 parts of peltate yam rhizome, 8 parts of stichopus japonicus, 10 parts of excoecaria venenata and 10 parts of rubus irenaeus focke. The clinical trial proves that the traditional Chinese medicine can safely and effectively treat hidradenitis suppurativa.

The invention relates to a high-efficiency extraction and cleaning production process for diosgenin, relates to the technical field of chemical industry and aims to solve the technical problems of low extraction efficiency, high production cost, difficulty in dehydration and concentration, serious pollution to environment and the like in the convention method for producing the diosgenin. The process comprises the following steps of: a) pretreating raw materials, namely crushing dioscorea zingiberensis; b) extracting dioscin, namely performing ultrasonic continuous countercurrent extraction on the crushed raw materials by using an alcohol water solvent, filtering to obtain filter residues and filtrate, and concentrating the filtrate to obtain a dioscin extract; c) performing enzymolysis or acid hydrolysis on the dioscin, namely performing the enzymolysis or the acid hydrolysis on the obtained dioscin extract; and d) extracting by using an organic solvent, namely performing countercurrent extraction on the obtained zymolyte or acid hydrolysate by using No.120 gasoline or No.6 gasoline, concentrating extract liquor, cooling for crystallizing, performing suction filtration and drying to obtain pure diosgenin.

A marinade for making braised meat is composed of a pork bone soup, pork skin jelly, white granulated sugar, anise, fructus amomi, round cardamom fruit, pepper fruit, kaempferiae, tangerine peel, long pepper, orange peel, nardostachys root, lesser galangal, katsumada galangal seeds, nutmeg, cloves, cortex acanthopanacis, dwarf lilyturf tuber, fennel, sandalwood, licorice roots northwest origin, grosvenor momordica fruit, agilawood, cassia twig, lysimachia sikokiana, areca seeds, apples, spices, herbo-viscid germander, dioscorea zingiberensis, costustoot, cape jasmine fruit, bay leaves, Chinese red pepper, radix angelicae, angelica sinensis, murraya paniculata and dried chilies. The produced marinade can be stored for a long time and can be directly used for making the braised meat which is delicious, and the operation is convenient and easy to master.

The invention obtains an endophyte of a dioscorea zingiberensis by steps of field sampling, sample surface sterilization, isolation of microorganism, liquid culture and the like; the strain is identified as bacillus licheniformis, which can produce diosgenin of the dioscorea zingiberensis and can be used as production strain for fermentation.

The invention belongs to the technical field of plant ingredient extraction, and discloses a method for preparing diosgenin from B acid DES (deep eutectic solvent) hydrolysis. Choline chloride and B acid are heated to a molten state so as to obtain a DES; dioscorea zingiberensis rhizome raw material powder, the DES and petroleum ether are added into a reactor; the temperature is raised to 100 to 130 DEG C; stirring and hydrolysis reaction are preformed; reaction liquid is subjected to filtering or centrifugal separation; a liquid petroleum ether phase is taken; petroleum ether recovered through evaporation; the diosgenin in the rest solid phase is extracted by using water saturation normal butanol solution; the normal butanol and the water are recovered through evaporation; the diosgenin is obtained. The characteristic that the B acid DES and the diosgenin can form strong hydrogen bonds is utilized, so that the DES can realize the selective extraction of the diosgenin from the raw materials for hydrolysis. The method has the advantages that the reaction selectivity is good; the pollution is low; the reaction conditions are mild; the problem of high pollution caused by simultaneoushydrolysis of cellulose and starch in the raw materials into sugar in a conventional hydrolysis process can be solved.

This invention relates to a method to efficiently extract diosgenin from the root and stem of dioscorea zingiberensis without fermentation, that is, a proper amount of water is added and the root and stem of dioscorea zingiberensis are pulverized. Fiber sediments and starch in the sludge are separated by a mechanical method and the pH value of the sludge is immediately adjusted to below 4.5 with acid. The adjusted and unfermented sludge is concentrated and acid hydrolyzed with the aid of hydrolysis agent. The hydrolyzed mixture is filtered to remove acid, neutralized, dried, extracted with organic solvent and crystallized so as to obtain diosgenin product.as fermentation is purposely inhibited and avoided during acid hydrolysis of the concentrated sludge in this invention, not only the stench problem is solved, but also the hydrolysis effect is greatly promoted. The extracted diosgenin product is of good crystallinity, high purity, white color and its melting point can be above 200 deg. C.

The invention relates to a method for extracting dioscorea zingiberensis diosgenin in traditional Chinese medicine peltate yam, a preparation agent, and the application of the dioscorea zingiberensis diosgenin in preparing drugs in prevention and treatment of cardiovascular diseases. The dioscorea zingiberensis diosgenin extraction method of the invention adopts enzyme to remove coarse impurities, and then uses weak-polar macroporous resin to extract the dioscorea zingiberensis diosgenin. The method of the invention can significantly improve the purity and extraction rate of dioscorea zingiberensis diosgenin to reduce the production cost. The dioscorea zingiberensis diosgenin of the invention can also prepare coated tablets, syrup, oral solution, drops or capsules; compared with the prior art, the dioscorea zingiberensis diosgenin prepared through the method of the invention has better efficacy and is conducive to the transport and storage. Clinical trials prove that the dioscorea zingiberensis diosgenin extracted in the invention has a significant effect on coronary heart disease, hyperlipidemia and other cardiovascular diseases.

The present invention relates to a new method for preparing diosgenin through microwave-assisted acidic ionic liquid catalysis, and belongs to the technical field of agricultural product deep processing and medicine. The method comprises: extracting the total saponin in the rhizome of dioscorea zingiberensis or other dioscoreaceae plants with an ethanol aqueous solution, carrying out catalytic hydrolysis on the saponin in a microwave reaction tank through the co-action of microwaves and an acidic ionic liquid to obtain diosgenin, cooling the reaction liquid, filtering, drying the filtration residue, extracting the diosgenin with petroleum ether, and removing water from the filtrate to obtain the ionic liquid, wherein the recovered ionic liquid can be recycled. According to the present invention, the acidic ionic liquid is used to replace the inorganic acid in the traditional hydrolysis method, such that the problem that a large amount of high-acidity chlorine-containing sewage is discharged is solved; the problems of long time consumption and complex production process of the biological conversion method and the enzyme method are overcome; with the microwave-assisted technology, the reaction time can be significantly shortened; the ionic liquid can be recycled so as to reduce the production cost; and the method is the efficient and green environmental protection diosgenin preparation method.

The invention relates to a dong-shaped Chinese potato endogenetic fusarin producing beauveria. The endogenetic fungal Dzf2 incorporating the invention is obtained from dong-shaped Chinese potato by endogenetic fungal isolation technology, and is classified and determined as fusarium by tiny organism. The bacterial strain has been preserved in china tiny organism culture preservation management committee commonness tiny organism local and accession identification serial number is CGMCC No.2340. The endogenetic fungal producing beauveria is first separated from dong-shaped Chinese potato and the antibacterial activity of beauveria is definituded. The object of the invention is to obtain savageness activity ingredient via plants endogenetic fungal resource.

The invention discloses a method for clean production of saponin from Dioscorea zingiberensis C. H. Wright. The method comprises the following steps of crushing a dry raw material of Dioscorea zingiberensis C. H. Wright, sieving the powder of Dioscorea zingiberensis C. H. Wright by a sieve of 20 meshes, carrying out methanol or ethanol extraction, carrying out concentration recovery of methanol or ethanol from the extract until an alcohol smell does not exist, putting the extract treated by the previous step into a container, carrying out microbial constant-temperature fermentation, adding hydrochloric acid or sulfuric acid into the container for acid hydrolysis, filtering to obtain crude saponin, carrying out crystallization and recrystallization of the crude saponin by petroleum ether, and carrying out vacuum drying to obtain a finished product. The method for clean production of saponin from Dioscorea zingiberensis C. H. Wright has the advantages of high yield, good color and good quality of saponin, and no pollution.

The invention belongs to the field of plant cultivation and discloses an artificial cultivation method of dioscorea zingiberensis. The artificial cultivation method of the dioscorea zingiberensis comprises steps of seed selection, soil preparation, seed sowing, frame erecting, weeding, fertilizer applying, water drainage, pest control, ridging and harvest. Seed stem preprocessing is performed before seed sowing, and a seed stem preprocessing method is that 50% of carbendazol wettable powder is diluted into 1000 times to soak seed stems for 30 minutes or water is utilized to soak the seed stems for 30 minutes. The fertilizer applying comprises base fertilizer applying and additional fertilizer applying, wherein base fertilizers are compound fertilizers of potassium sulfate, and additional fertilizers are leaf fertilizers. The artificial cultivation method of the dioscorea zingiberensis overcomes the shortcoming that the existing artificial cultivation method of the dioscorea zingiberensis is non-standard in cultivation technology and low in harvest yield and saponin content and results in tuber rot due to blind fertilization, and is standard in cultivation technology, capable of avoiding tuber rot and high in harvest yield and saponin content.

The invention relates to a cultivation method of dioscorea zingiberensis C.H.Wright. According to the cultivation method of the dioscorea zingiberensis C.H.Wright, the yield is high, and the sowing quantity is small. The cultivation method of the dioscorea zingiberensis C.H.Wright comprises the steps of selecting land, carrying out land preparation, selecting seeds, carrying out seeds soaking, carrying out primary planting, carrying out field planting, carrying out water and fertilizer management, carrying out rod insertion and frame erection, carrying out harvesting, and carrying out storage. According to the cultivation method of the dioscorea zingiberensis C.H.Wright, due to the fact that the scientific and reasonable steps of land selection, land preparation, seed selection, seed soaking, primary planting, field planting, water and fertilizer management, rod insertion, frame erection, harvesting and storage are adopted, the yield of the planted dioscorea zingiberensis C.H.Wright can be increased to 3000 kilograms per mu from 2000 kilograms per mu; in addition, the input quantity of a seed source can be reduced to 100 kilograms per mu from 300 kilograms per mu in the prior art, the net production value per mu of the dioscorea zingiberensis C.H.Wright can be greatly increased, the net production value per mu can be increased by 2700 yuan RMB every year, and the economic benefit of farmers for planting the dioscorea zingiberensis C.H.Wright can be greatly improved.

This invention provides a new technology of cleaning production that extract sapogenin from dioscorea zingiberensis wright. It uses rhizome of fresh dioscorea zingiberensis or fresh dioscorea nipponica that are 2 -3 perennials as raw material. Its technology is that: raw material-microwave processing-ultramicro-pulverization-dynamic state continuous extracting-rectification-hydrolization-crude sapogenin-dissolving adsorption for purification-rectification-crystallization-drying-depurating sapogenin. This invention provides a new technology that produces diosgenin. Its technology is advanced, investment is little, material expenditure is low, production yield is high, quality is satisfactory, production period is so short that extracting technology can complete in 20 hours. It has no pollution. Content of COD in the water is less than 300mg / l.

The invention relates to a traditional Chinese medicine for treating melanosis coli. The Chinese medicine is prepared from the following medicinal materials in parts by weight: 10 parts of ixeris japonica, 12 parts of wild flax, 14 parts of rhizoma polygoni paleacei, 14 parts of cirrhopetalum, 10 parts of dried glomeris nipponica kishida, 12 parts of mango, 12 parts of eria graminifolia, 15 parts of viburnum utile hemsl, 8 parts of earthworm, 6 parts of potentilla limprichtii, 10 parts of clausena excavata burm, 8 parts of dioscorea zingiberensis, 12 parts of christia constricta, 10 parts of sibbaldia procumbens, 12 parts of root of himalayan snakegourd, 10 parts of chimonanthus nitens, 13 parts of aristolochia mollis dunn, 12 parts of willowweed and 12 parts of Chinese actinidia root. Clinical trial shows that the traditional Chinese medicine is capable of safely and effectively treating melanosis coli.

The invention relates to a method for purifying yellow pigment in yellow ginger, comprising the following steps of scrubbing, slicing, drying and pulverizing wet yellow ginger; adding ethanol solution for extraction through reflux at constant temperature; removing ethanol by rotation and evaporation; drying to obtain a crude product of dioscorea zingiberensis pigment; preparing the crude product of dioscorea zingiberensis pigment into dioscorea zingiberensis pigment solution; enabling the dioscorea zingiberensis pigment solution to pass through a macroporous resin column, and eluting with distilled water to remove impurities ; carrying out elution with ethanol; and concentrating and drying the obtained ethanol eluent to obtain a refined product of dioscorea zingiberensis pigment. In the invention, the macroporous resin column is utilized to specifically absorb yellow pigment in yellow ginger, and elution is carried out by using ethanol so as to achieve the purpose of purifying dioscorea zingiberensis pigment. The method has the advantages of easy adsorption and desorption, large adsorption capacity and high color value of the product with the pigment yield rate of 4.48 percent and the color value of 114.5.

The invention discloses an effective and low-energy-consumption rapid propagation method of dioscorea zingiberensis. The method comprises steps as follows: (1) stems of an excellent medicinal dioscorea zingiberensis plant are selected as explants, the explants are disinfected and then cultured in an agar solid culture medium containing MS, 6-BA (0.01-0.05 mg / L), paclobutrazol (3.0-10.0 mg / L) and chlormequat chloride (3.0-10.0 mg / L) and having the content of components excluding inositol unchanged and the content of inositol doubled, and a large quantity of small bud clusters are induced; (2) the cluster buds obtained in the step (1) are cut off and divided into clusters, each cluster comprises five buds, an agar solid culture medium containing MS, NAA (0.02-0.6 mg / L), IAA (0.02-0.6 mg / L), paclobutrazol (0.5-5.0 mg / L) and chlormequat chloride (0.5-5.0 mg / L) and having the content of components excluding inositol unchanged and the content of inositol doubled is inoculated with the buds for proliferation and rooting. The method is good in cultivation effect, high in proliferation coefficient, good in stability and low in production cost and is a rapid propagation method for obtaining a large quantity of test-tube plantlets within the shortest time.

The invention discloses a process for extracting water-soluble saponin from Mexican Peltate yam rahizome which comprises, using Mexican Peltate yam rahizome as raw material, comminuting and carrying fine grinding, sieving, deslagging, depositing the filtrate under controlled temperature, concentrating the upper layer liquid, charging ethanol and stewing, filtering and reclaiming ethanol, concentrating, purifying through adsorptive separation, finally concentrating and drying again.

The invention discloses an alcohol-free extraction method for dioscin. The solubilization effect of a surfactant OP-10 is utilized; water is taken as an extractant, so the cost is low; the pollution is avoided in the extraction process; the dioscin is extracted from Dioscorea zingiberensis Wright; the coating effect of the surfactant OP-10 on the dioscin is damaged when the dioscin is hydrolyzed to form diosgenin by acid; and a hydrolysate of the OP-10 is separated from the diosgenin through a selective extractant, namely gasoline or petroleum ether. The concentration of the OP-10 is 1.5 percent, the OP-10 is sold at 19,000yuan / ton, 7.5 tons of OP-10 is required for extracting 1 ton of dioscin, and the cost is 142.5 thousand yuan and is 40 percent of the cost of an ethanol extraction method, so the extraction cost for the dioscin is low. After the dioscin is extracted from the Dioscorea zingiberensis Wright, the inhibition effect of the dioscin on saccharomycetes is relieved; fermented mash with the alcoholic strength of 10.5 percent can be obtained through fermentation; industrial alcohol can be obtained through distillation; and after fermentation, the content of proteins is improved, so the Dioscorea zingiberensis Wright can be taken as a feed.