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Method for cleaning and producing dioscorea zingiberensis saponin with microbial technology adopted

A microbial technology and a technology for turmeric saponin, applied in the field of clean production of turmeric saponin using microbial technology, can solve the problems of long pretreatment time and increase the difficulty of membrane separation, improve yield, realize clean production and high-value synthesis The effect of utilization, easy industrial application

Active Publication Date: 2013-04-24
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology can greatly reduce the amount of acid and organic solvents, and realize the clean production of turmeric saponin and co-production of organic acids by using turmeric raw materials. The mixture of external fermentation products and water-soluble turmeric saponins in the fermentation broth increases the difficulty of membrane separation and other problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 comprises the following steps:

[0033] 1. Pretreatment steps of turmeric raw materials

[0034] 500 grams of turmeric roots are dried and crushed to 100 mesh, steam exploded for 15 minutes at a temperature of 120-130 °C and a pressure of 0.1-0.2 MPa, and cooled to room temperature;

[0035] 2. Microbial treatment release step of turmeric saponin

[0036] The above dry turmeric powder is mixed according to dry powder of turmeric: water = 1:5 (w / v), add water and stir evenly; then add 10% seed solution, under the conditions of pH value 6.0 and temperature 37°C, the stirring speed is 150rpm, and the fermentation treatment ends after 10h.

[0037] The seed liquid is an ordinary LB medium, that is, add 10 g of peptone, 5 g of yeast powder, and 10 g of sodium chloride per liter of water to adjust the pH value to 7.0, mix it into a seed medium and sterilize it, and cool to room temperature to obtain sterile seeds culture medium: inoculate the bacterial strains ...

Embodiment 2

[0051] Embodiment 2 comprises the following steps:

[0052] 1. Pretreatment steps of turmeric raw materials

[0053] 1000 grams of turmeric roots are dried and crushed to 60 mesh.

[0054] 2. Microbial treatment release step of turmeric saponin

[0055] According to turmeric dry powder: water = 1: 10 (w / v), add water and mix evenly; after cooling, inoculate 10% seed liquid, under the condition of pH value 6.0, temperature 37 ℃, stirring speed 150rpm, fermentation treatment ends after 6h.

[0056] The seed liquid is an ordinary LB medium, that is, add 10 g of peptone, 5 g of yeast powder, and 10 g of sodium chloride per liter of water to adjust the pH value to 7.0, mix it into a seed medium and sterilize it, and cool to room temperature to obtain sterile seeds Culture medium: Inoculate two bacterial strains capable of producing cellulase, hemicellulose, and pectinase into the sterile seed medium, stir at a pH value of 7 and a temperature of 37°C at a stirring speed of 120rpm ...

Embodiment 3

[0070] Embodiment 3 comprises the following steps:

[0071] 1. Pretreatment steps of turmeric raw materials

[0072] After washing and removing impurities from fresh turmeric, take 20 kg of fresh turmeric and steam-explode it for 20 minutes at a temperature of 120-130°C and a pressure of 0.1-0.2 MPa, and cool to room temperature.

[0073] 2. Microbial treatment release step of turmeric saponin

[0074] Crush the pretreated turmeric tubers into a slurry, add water and stir evenly according to fresh turmeric: water = 1:2 (w / v); then add 10% seed liquid, and stir at a pH value of 6.0 and a temperature of 37°C The rotating speed is 150rpm, and the fermentation treatment ends after 10h.

[0075] The seed liquid is an ordinary LB medium, that is, add 10 g of peptone, 5 g of yeast powder, and 10 g of sodium chloride per liter of water to adjust the pH value to 7.0, mix it into a seed medium and sterilize it, and cool to room temperature to obtain sterile seeds Culture medium: Inoc...

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Abstract

The invention provides a method for cleaning and producing dioscorea zingiberensis saponin with microbial technology adopted, and belongs to the field of bioengineering. The method solves the problems that in the existing dioscorea zingiberensis industry, wastewater and waste residues are large in drainage amount, and dioscorea zingiberensis starch is not utilized effectively. The method includes the following steps: releasing dioscorea zingiberensis saponin with microorganism adopted firstly, obtaining fermentation liquor containing the dioscorea zingiberensis saponin and the starch; and then processing the starch in the fermentation liquor with a two-enzyme method adopted and obtaining hydrolyzed sugar, and further releasing the dioscorea zingiberensis saponin; conducting solid-liquid separation on dioscorea zingiberensis mash; gathering the hydrolyzed sugar and the dioscorea zingiberensis saponin with microfiltration and nanofiltration membrane separation adopted in sequence, or obtaining intracellular products and dioscorea zingiberensis saponin through specific microbial fermentation separation, and obtaining the dioscorea zingiberensis saponin by hydrolysis of glycosidase and a small amount acid and extraction of a small amount of organic solvent on concentrated dioscorea zingiberensis saponin solution. According to the method, the yield coefficient of the dioscorea zingiberensis saponin is high (90-95%), wastewater quantity is little in the process of production, degree of contamination is low, microbial treatment is low in cost and short in use time, the dioscorea zingiberensis starch is recycled fully, and the method has a great industrial application promotion value in the dioscorea zingiberensis industry.

Description

technical field [0001] The invention belongs to the fields of bioengineering and biochemical industry, and in particular relates to a method for cleanly producing turmeric saponin by utilizing microbial technology. The method secretes cellulase, pectinase, hemicellulase and xylanase with high activity and starch Microorganisms with low enzyme activity pretreat turmeric raw materials, and then use double-enzyme method to hydrolyze starch at high temperature to obtain hydrolyzed sugar and at the same time inactivate the microorganisms used for pretreatment; then combine membrane separation to cleanly produce hydrolyzed sugar and turmeric saponin, or use intracellular products The microbial fermentation directly utilizes the hydrolyzed sugar, and realizes the separation of turmeric saponin and hydrolyzed sugar while obtaining intracellular products. Background technique [0002] Turmeric, scientific name Dioscorea shield leaf, perennial herb, is a unique medicinal material in m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12P7/06C12P7/64C12P7/46C12P7/56
CPCY02E50/10
Inventor 余龙江魏蜜余潘潘敖明章金文闻
Owner HUAZHONG UNIV OF SCI & TECH
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